Potential for biomolecular imaging with femtosecond X-ray pulses

Abstract

Sample damage by X-rays and other radiation limits the resolution of structural studies on non-repetitive and non-reproducible structures such as individual biomolecules or cells1. Cooling can slow sample deterioration, but cannot eliminate damage-induced sample movement during the time needed for conventional measurements1,2. Analyses of the dynamics of damage formation3,4,5 suggest that the conventional damage barrier (about 200 X-ray photons per Å2 with X-rays of 12 keV energy or 1 Å wavelength2) may be extended at very high dose rates and very short exposure times. Here we have used computer simulations to investigate the structural information that can be recovered from the scattering of intense femtosecond X-ray pulses by single protein molecules and small assemblies. Estimations of radiation damage as a function of photon energy, pulse length, integrated pulse intensity and sample size show that experiments using very high X-ray dose rates and ultrashort exposures may provide useful structural information before radiation damage destroys the sample. We predict that such ultrashort, high-intensity X-ray pulses from free-electron lasers6,7 that are currently under development, in combination with container-free sample handling methods based on spraying techniques, will provide a new approach to structural determinations with X-rays.

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Figure 1: Ionization of a lysozyme molecule in intense X-ray pulses.
Figure 2: Explosion of T4 lysozyme (white, H; grey, C; blue, N; red, O; yellow, S) induced by radiation damage.
Figure 3: Elastic scattering from a variety of samples.
Figure 4: The landscape of damage tolerance.

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Acknowledgements

We are grateful to G. Faigel, S. Hovmöller, A. Szöke, M. van Heel and A. Pratt for discussions. This work was supported by the Swedish Research Councils NFR and TFR as well as the EU-BIOTECH programme.

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Correspondence to Janos Hajdu.

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Neutze, R., Wouts, R., van der Spoel, D. et al. Potential for biomolecular imaging with femtosecond X-ray pulses. Nature 406, 752–757 (2000). https://doi.org/10.1038/35021099

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