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Oncogene inactivation in a mouse model

Nature volume 406, pages 473–474 (2000)Cite this article

Tissue invasion by leukaemic cells is stalled by loading them with a designer ribozyme.

Abstract

Chronic myelogenous leukaemia (CML) is a haematopoietic malignant disease associated with the expression of a chimaeric BCR–ABL gene1,2. We have designed an allosterically controllable ribozyme that specifically cleaves BCR–ABL messenger RNA and induces apoptosis in cultured CML cells3, and here we test it as a possible treatment of CML in a mouse model. We find that this ribozyme completely inhibits tumour-cell infiltration in these mice. To our knowledge, this is the first application of an artificial, allosterically controllable enzyme in animals, opening up the possibility of using ribozyme technology in the treatment of CML.

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Figure 1: The antitumour effects of the maxizyme in a murine model of chronic myelogenous leukaemia (CML).

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Authors and Affiliations

  1. Department of Hematology/Oncology Institute of Medical Science, University of Tokyo, Minato-ku , Tokyo, 108-8539, Japan

    Tsuyoshi Tanabe, Kenzaburo Tani & Shigetaka Asano

  2. National Institute for Advanced Interdisciplinary Research, 1-1-4 Higashi, Tsukuba Science City , 305-8562, Japan

    Tsuyoshi Tanabe, Tomoko Kuwabara, Masaki Warashina & Kazunari Taira

  3. Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba Science City, 305-8572, Japan

    Tomoko Kuwabara & Masaki Warashina

  4. Department of Chemistry and Biotechnology Graduate School of Engineering, University of Tokyo, Hongo, Tokyo, 113-8656, Japan

    Kazunari Taira

Authors
  1. Tsuyoshi Tanabe
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  2. Tomoko Kuwabara
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  3. Masaki Warashina
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  4. Kenzaburo Tani
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  5. Kazunari Taira
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  6. Shigetaka Asano
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Correspondence to Kazunari Taira.

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Tanabe, T., Kuwabara, T., Warashina, M. et al. Oncogene inactivation in a mouse model. Nature 406, 473–474 (2000). https://doi.org/10.1038/35020190

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