Letter | Published:

The S receptor kinase determines self-incompatibility in Brassica stigma

Nature volume 403, pages 913916 (24 February 2000) | Download Citation

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Abstract

The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self-incompatibility in Brassica is sporophytically controlled by the polymorphic S locus1. Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma2,3,4, and analyses of self-compatible lines5,6,7 of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG 28 transgene separately, that expression of SRK28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S28 pollen was enhanced by SLG 28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response.

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Acknowledgements

We thank T.-h. Kao for his critical review and editing of the manuscript. This work was supported in part by Grants-in-Aid for Special research on Priority Areas from the Ministry of Education, Science, Culture and Sports, Japan.

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Affiliations

  1. *Research Institute of Seed Production Co., Ltd. 6-6-3 Minamiyoshinari, Aoba-ku, Sendai 989-3204, Japan

    • Takeshi Takasaki
    • , Katsunori Hatakeyama
    •  & Kokichi Hinata
  2. †Faculty of Agriculture, Kobe University , Nada-ku, Kobe 657-8501, Japan

    • Takeshi Takasaki
  3. ‡Division of Natural Science, Osaka Kyoiku University, Kashiwara, Osaka 582-8582 , Japan

    • Go Suzuki
  4. §Faculty of Agriculture, Iwate University , Morioka 020-8550, Japan

    • Masao Watanabe
  5. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0101 , Japan

    • Akira Isogai

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Correspondence to Takeshi Takasaki.

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https://doi.org/10.1038/35002628

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