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The S receptor kinase determines self-incompatibility in Brassica stigma

Abstract

The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self-incompatibility in Brassica is sporophytically controlled by the polymorphic S locus1. Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma2,3,4, and analyses of self-compatible lines5,6,7 of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG 28 transgene separately, that expression of SRK28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S28 pollen was enhanced by SLG28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response.

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Figure 1: Vector construction and detection of transgenes.
Figure 2: RNA blot analysis of transcription of the endogenous SRK28 gene and the SRK28 transgene.
Figure 3: Expression of the SRK28 and SLG28 transgenes.

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Acknowledgements

We thank T.-h. Kao for his critical review and editing of the manuscript. This work was supported in part by Grants-in-Aid for Special research on Priority Areas from the Ministry of Education, Science, Culture and Sports, Japan.

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Correspondence to Takeshi Takasaki.

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Takasaki, T., Hatakeyama, K., Suzuki, G. et al. The S receptor kinase determines self-incompatibility in Brassica stigma. Nature 403, 913–916 (2000). https://doi.org/10.1038/35002628

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