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Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase

Abstract

THE fission yeast wee1+ gene product is a dose-dependent, negative regulator of entry into mitosis1,2. wee1+ encodes a protein of relative molecular mass 107,000 (Mr 107K), the C-terminal third of which has strong similarities with the serine/threonine protein kinase family2,3. Here we report that p107wee1 immune complexes phosphorylate p107wee1 equally on serine and tyrosine residues, and also phosphorylate an exogenous substrate, angiotensin II, on tyrosine. Both kinase activities are attributable to p107wee1 because they are also observed when weel + is expressed in heterologous systems; both are abolished by a point mutation in the ATP-binding domain, and both behave like an asymmetric monomer of Mr 114K on gel filtration and density-gradient centrifugation. Thus the wee1+ gene product is representative of a novel class of protein kinase that phosphorylates both serine and tyrosine residues.

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References

  1. 1

    Nurse, P. Nature 334, 503–508 (1990).

  2. 2

    Russell, P. & Nurse, P. Cell 49, 559–567 (1987).

  3. 3

    Hanks, S. K., Quinn, A. M., & Hunter, T. Science 241, 42–52 (1988).

  4. 4

    Russell, P., Moreno, S. & Reed, S. I. Cell 57, 295–303 (1989).

  5. 5

    Smith, G. E., Summers, M. D. & Frazer, M. J. Molec. cell. Biol. 3, 2156–2165 (1983).

  6. 6

    Siegel, L. M. & Monty, K. J. Biochim. biophys. Acta 112, 346–362 (1966).

  7. 7

    Gould, K. L. & Nurse, P. Nature 342, 39–45 (1989).

  8. 8

    Moreno, S., Klar, A. & Nurse, P. Meth. Enzym. 56, 795–823 (1991).

  9. 9

    Maizel, J. V. in Fundamental Techniques in Virology (eds Habel, K. H. & Salzman, N. P. 334–362 (Academic, New York, 1969).

  10. 10

    Burnette, W. N. Analyt. Biochem 112, 195–203 (1981).

  11. 11

    Cooper, J. A., Sefton, B. M. & Hunter, T. Meth. Enzym. 99, 387–402 (1983).

  12. 12

    Luckow, V. A. & Summers, M. D. Virology 170, 31–39 (1989).

  13. 13

    Summers, M. & Smith G. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures (Texas Agricultural Experiment Station Bulletin No. 1555, College Station, Texas, 1987).

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