Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes

Nature volume 349, pages 487–493 (1991)Cite this article

Abstract

The product of the yeast PRP22 gene acts late in the splicing of yeast pre-messenger RNA, mediating the release of the spliced mRNA from the spliceosome. The predicted PRP22 protein sequence shares extensive homology with that of PRP2 and PRP16 proteins, which are also involved in nuclear pre-mRNA splicing. The homologous region contains sequence elements characteristic of several demonstrated or putative ATP-dependent RNA helicases. A putative RNA-binding motif originally identified in bacterial ribosomal protein SI and Escherichia coli polynucleotide phos-phorylase has also been found in PRP22.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Brody, E. & Abelson, J. Science 228, 963–967 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Frendewey, D. & Keller, W. Cell 42, 355–367 (1985).

    Article  CAS  PubMed  Google Scholar 

  3. Grabowski, P. J., Seiler, S. R. & Sharp, P. A. Cell 42, 345–353 (1985).

    Article  CAS  PubMed  Google Scholar 

  4. Green, M. R. A. Rev. Genet. 20, 671–708 (1986).

    Article  CAS  Google Scholar 

  5. Padgett, R. A., Grabowski, P. J., Konarska, M. M., Seiler, S. & Sharp, P. A. A. Rev. Biochem. 55, 1119–1150 (1986).

    Article  CAS  Google Scholar 

  6. Cech, T. R. & Bass, B. L. A. Rev. Biochem. 55, 599–629 (1986).

    Article  CAS  Google Scholar 

  7. Sharp, P. A. Science 235, 766–771 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Pikielny, C. W., Rymond, B. C. & Rosbash, M. Nature 324, 341–345 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Cheng, S. C. & Abelson, J. Genes Dev. 1, 1014–1027 (1987).

    Article  CAS  PubMed  Google Scholar 

  10. Konarska, M. M. & Sharp, P. A. Cell 49, 763–774 (1987).

    Article  CAS  PubMed  Google Scholar 

  11. Lin, R. J., Lustig, A. J. & Abelson, J. Genes Dev. 1, 7–18 (1987).

    Article  CAS  PubMed  Google Scholar 

  12. Sawa, H., Ohno, M., Sakamoto, H. & Shimura, Y. Nucleic Acids Res. 16, 3157–3164 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Vijayraghavan, U. & Abelson, J. Molec. cell. Biol. 10, 324–332 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Schwer, B. & Guthrie, C. Nature 349, 494–499 (1991).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Maniatis, T. & Reed, R. Nature 325, 673–678 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Guthrie, C. & Patterson, B. A. Rev. Genet. 22, 387–419 (1988).

    Article  CAS  Google Scholar 

  17. Steitz, J. A. et al. in Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles (ed. Birnstiel, M. L.) 115–154 (Springer-Verlag, Berlin, 1988).

    Book  Google Scholar 

  18. Lührman, R. in Structure and Function of Major and Minor Small Nuclear ribonucleoprotein Particles (ed. Birnsteil, M. L.) 71–99 (Springer-Verlag, Berlin, 1988).

    Book  Google Scholar 

  19. Vijayraghavan, U. & Abelson, J. Nucleic Acids molec. Biol. 3, 197–215 (1989).

    Article  Google Scholar 

  20. Woolford, J. L. Yeast 5, 439–458 (1989).

    Article  CAS  PubMed  Google Scholar 

  21. Ruby, S. W. & Abelson, J. Trends Genet. 7, 1–9 (1991).

    Article  Google Scholar 

  22. Lustig, A. J., Lin, R. J. & Abelson, J. Cell 47, 953–963 (1986).

    Article  CAS  PubMed  Google Scholar 

  23. Lossky, M. G., Anderson, J., Jackson, S. P. & Beggs, J. D. Cell 51, 1019–1026 (1987).

    Article  CAS  PubMed  Google Scholar 

  24. Pinto, A. L. & Steitz, J. A. Proc. natn. Acad. Sci. U.S.A. 86, 8742–8746 (1989).

    Article  ADS  CAS  Google Scholar 

  25. Anderson, G. J., Bach, M., Lührmann, R. & Beggs, J. D. Nature 342, 819–821 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Banroques, J. & Abelson, J. Molec. cell. Biol. 9, 3710–3719 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Bjorn, S. P., Soltyk, A., Beggs, J. D. & Friesen, J. D. Molec. cell. Biol. 9, 3698–3709 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Chang, T. H., Clark, M. W., Lustig, A. J., Cusick, M. E. & Abelson, J. Molec. cell. Biol. 8, 2379–2393 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Hodgman, T. C. Nature 333, 22–23 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Lane, D. Nature 334, 478 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Gorbalenya, E., Koonin, E. V., Donchenko, A. P. & Blinov, V. M. Nucleic Acids Res. 17, 4713–4730 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Gorbalenya, A. E. & Koonin, E. V. Nucleic Acids Res. 17, 8413–8440 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Linder, P. et al. Nature 337, 121–122 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Chang, T. H., Arenas, J. & Abelson, J. Proc. natn. Acad. Sci. U.S.A. 87, 1571–1575 (1990).

    Article  ADS  CAS  Google Scholar 

  35. Dalbadie-McFarland, G. & Abelson, J. Proc. natn. Acad. Sci. U.S.A. 87, 4236–4240 (1990).

    Article  ADS  CAS  Google Scholar 

  36. Burgess, S., Couto, J. R. & Guthrie, C. Cell 60, 705–717 (1990).

    Article  CAS  PubMed  Google Scholar 

  37. Chen, J. H. & Lin, R. J. Nucleic Acids Res. 18, 6447 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Vijayraghavan, U., Company, M. & Abelson, J. Genes Dev. 3, 1206–1216 (1989).

    Article  CAS  PubMed  Google Scholar 

  39. Lamond, A. I., Konarska, M. M. & Sharp, P. A. Genes Dev. 1, 532–543 (1987).

    Article  CAS  PubMed  Google Scholar 

  40. Sikorski, R. S. & Heiter, P. Genetics 122, 19–27 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  41. Pearson, W. R. & Lipman, D. J. Proc. natn. Acad. Sci. U.S.A. 85, 2444–2448 (1988).

    Article  ADS  CAS  Google Scholar 

  42. Schnier, J. et al. Proc. natn. Acad. Sci. U.S.A. 79, 1008–1011 (1982).

    Article  ADS  CAS  Google Scholar 

  43. Subramanian, A. R. Prog. nucleic Acids. Res. molec. Biol. 28, 101–142 (1983).

    Article  CAS  Google Scholar 

  44. Regnier, P., Grunberg-Manago, M. & Portier, C. J. biol. Chem. 262, 63–68 (1987).

    CAS  PubMed  Google Scholar 

  45. Subramanian, A. R. Essays biochem. 21, 45–85 (1985).

    CAS  PubMed  Google Scholar 

  46. Gribskov, M., Lüthy, R. & Eisenberg, D. Meth. Enzymol. 183, 146–159 (1989).

    Article  Google Scholar 

  47. Sands, J. F., Cummings, H. S., Sacerdot, C., Dondon, L., Grunberg-Manago, M. & Hershey, J. W. B. Nucleic Acids Res. 15, 5157–5168 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Couto, J. R., Tamm, J., Parker, R. & Guthrie, C. Genes Dev. 1, 445–455 (1987).

    Article  CAS  PubMed  Google Scholar 

  49. Lain, S., Riechmann, J. L., Martin, M. T. & Garcia, J. A. Gene 82, 357–362 (1989).

    Article  CAS  PubMed  Google Scholar 

  50. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO. J. 1, 945–951 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Fry, D. C., Kuby, S. A. & Mildvan, A. S. Proc. natn. Acad. Sci. U.S.A. 83, 907–911 (1986).

    Article  ADS  CAS  Google Scholar 

  52. Ray, B. K. et al. J. biol. Chem. 260, 7651–7658 (1985).

    CAS  PubMed  Google Scholar 

  53. Rozen, F. et al. Molec. cell. Biol. 10, 1134–1144 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Hirling, H., Scheffner, M., Restle, T. & Stahl, H. Nature 339, 562–564 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  55. Lain, S., Riechmann, J. L. & Garcia, J. A. Nucleic Acids Res. 18, 7003–7006 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. Lain, S., Martin, M. T., Riechmann, J. L. & Garcia, J. A. J. Virol. 65, 1–6 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  57. Seraphin, B., Simon, M., Boulet, A. & Faye, G. Nature 337, 84–87 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  58. Schnier, J. et al. Nucleic Acids Res. 16, 3075–3089 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Schnier, J. & Faist, G. Molec. gen. Genet. 200, 476–481 (1985).

    Article  CAS  PubMed  Google Scholar 

  60. Umesono, K., Inokuchi, H., Ohyama, K. & Ozeki, H. Nucleic Acids Res. 12, 9551–9565 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  61. Sijben-Mueller, G., Hallick, R. B., Alt, J., Westhoff, P. & Herrmann, R. G. Nucleic Acids Res. 14, 1029–1044 (1986).

    Article  CAS  Google Scholar 

  62. Cigan, A. M., Pabich, E. K., Feng, L. & Donahue, T. F. Proc. natn. Acad. Sci. U.S.A. 86, 2784–2788 (1989).

    Article  ADS  CAS  Google Scholar 

  63. Ernst, H., Duncan, R. F. & Hershey, J. W. B. J. biol. Chem. 262, 1206–1212 (1987).

    CAS  PubMed  Google Scholar 

  64. Lain, S., Riechmann, J. L. & Garcia, J. A. Virus Res. 13, 157–172 (1989).

    Article  CAS  PubMed  Google Scholar 

  65. Collett, M. S. et al. Virology 165, 191–199 (1988).

    Article  CAS  Google Scholar 

  66. Mackow, E. et al. Virology 159, 217–228 (1987).

    Article  CAS  PubMed  Google Scholar 

  67. Nielsen, P. J., McMaster, G. K. & Trachsel, H. Nucleic Acids Res. 13, 6867–6880 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

    Mahshid Company, Jaime Arenas & John Abelson

Authors
  1. Mahshid Company
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaime Arenas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John Abelson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Company, M., Arenas, J. & Abelson, J. Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes. Nature 349, 487–493 (1991). https://doi.org/10.1038/349487a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/349487a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing