Abstract
REGULATION of ion transport by phosphorylation and G proteins occurs in several epithelial and non-epithelial cell plasma membranes1–5. It is not known whether transporters on intracellular membranes are target sites for second messengers. Here we present direct evidence that a chloride conductance in endocytic vesicles from rabbit proximal tubule is activated by phosphorylation through a cyclic AMP-dependent protein kinase. To measure chloride transport, endocytic vesicles were labelled in vivo with a Cl−-sensitive fluorescent indicator6–8. It was found that labelled endosomes contained an inward proton pump and a chloride conductance, but no ion-coupled chloride transport, and that the chloride conductance was regulated by protein kinase A. These results, taken together with measurements of chloride effects on ATP-dependent acidification, suggest that endosomal pH can be controlled by phosphorylation of a stilbene-sensitive conductive chloride transporter.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Demarest, J. R., Loo, D. D. F. & Sachs, G. Science 245, 402–404 (1989).
Bahinski, A., Nairn, A. C., Greengard, P. & Gadsby, D. C. Nature 340, 718–721 (1989).
Li, M. et al. Nature 331, 358–360 (1988).
Schoumacher, R. A. et al. Nature 330, 752–754 (1987).
Brown, A. M. & Birnbaumer, L. Am. J. Physiol. 254, H401–H410 (1988).
Verkman, A. S. Am. J. Physiol. 259, C375–C388 (1990).
Pearce, D. & Verkman, A. S. Biophys. J. 55, 1251–1259 (1989).
Chao, A. C., Dix, J. A., Sellers, M. C. & Verkman, A. S. Biophys. J. 56, 1071–1081 (1989).
Rodman, J. S., Seidman, L. & Farquhar, M. G. J. Cell Biol. 102, 77–87 (1986).
Verkman, A. S., Lencer, W. I., Brown, D. & Ausiello, D. A. Nature 333, 268–269 (1988).
Shi, L.-B. & Verkman, A. S. J. gen. Physiol. 94, 1101–1115 (1989).
Ye, R., Shi, L.-B., Lencer, W. I. & Verkman, A. S. J. gen. Physiol. 93, 885–902 (1989).
Barasch, J., Gershon, M. D., Nunez, E. A., Tamir, H. & Al-Awqati, Q. J. Cell Biol. 107, 2137–2147 (1988).
Van Dyke, R. W. J. biol. Chem. 263, 2603–2611 (1988).
Mellman, I., Fuchs, R. & Helenius, A. Rev. Biochem. 55, 663–700 (1986).
Cain, C. C., Sipe, D. M. & Murphy, R. F. Proc. natn. Acad. Sci. U.S.A. 86, 544–548 (1989).
Fuchs, R., Schmid, S. & Mellman, I. Proc. natn. Acad. Sci. U.S.A. 86, 539–543 (1989).
Fuchs, R., Male, P. & Mellman, I. J. biol. Chem. 264, 2212–2220 (1989).
Lencer, W. I., Weyer, P., Verkman, A. S., Ausiello, D. A. & Brown, D. Am. J. Physiol. 258, C309–C317 (1990).
O'Grady, S. M., DeJonge, H. R., Vaandrager, A. B. & Field, M. Am. J. Physiol. 254, C115–C121 (1988).
Marunaka, Y. & Eaton, D. J. gen. Physiol. 95, 773–789 (1990).
Sabolic, I., Haase, W. & Burckhardt, G. Am. J. Physiol. 248, F835–F844 (1985).
Barthelson, R. & Widdicombe, J. J. clin. Invest. 80, 1799–1802 (1987).
Hopkins, C. R., Gibson, A., Shipman, M. & Miller, R. Nature 346, 335–339 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bae, HR., Verkman, A. Protein kinase A regulates chloride conductance in endocytic vesicles from proximal tubule. Nature 348, 637–639 (1990). https://doi.org/10.1038/348637a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/348637a0
This article is cited by
-
Bradykinin-induced chloride conductance in murine proximal tubule epithelial cells
Molecular and Cellular Biochemistry (2007)
-
Chemiosmotic coupling of ion transport in the yeast vacuole: Its role in acidification inside organelles
Journal of Bioenergetics and Biomembranes (1994)
-
Altered membrane ionic permeability in a rat model of chronic renal failure
Kidney International (1992)
-
Acidification indication
Nature (1991)
-
Back to the chloride channel
Nature (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.