Letter | Published:

Phage antibodies: filamentous phage displaying antibody variable domains

Nature volume 348, pages 552554 (06 December 1990) | Download Citation

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Abstract

NEW ways of making antibodies have recently been demonstrated using gene technology. Immunoglobulm variable (V) genes are amplified from hybridomas or B cells using the polymerase chain reaction, and cloned into expression vectors. Soluble antibody fragments secreted from bacteria are then screened for binding activities (see ref. 1 for review). Screening of V genes would, however, be revolutionized if they could be expressed on the surface of bacteriophage. Phage carrying V genes that encode binding activities could then be selected directly with antigen. Here we show that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage (one in a million) can be isolated after affinity chromatography.

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Author information

Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road,Cambridge CB2 2QH, UK

    • John McCafferty
    • , Andrew D. Griffiths
    •  & Greg Winter
  2. Cambridge Antibody Technology Ltd, Daly Research Laboratories,Babraham Hall, Cambridge, CB2 4AT, UK

    • John McCafferty
    •  & David J. Chiswell

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DOI

https://doi.org/10.1038/348552a0

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