Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domains

Nature volume 348, pages 411–418 (1990)Cite this article

Abstract

The structure of an N-terminal fragment of CD4 has been determined to 2.4 Å resolution. It has two tightly abutting domains connected by a continuous β strand. Both have the immunoglobulin fold, but domain 2 has a truncated β barrel and a non-standard disulphide bond. The binding sites for monoclonal antibodies, class II major histocom-patibility complex molecules, and human immunodeficiency virus gp120 can be mapped on the molecular surface.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. Proc. natn. Acad. Sci. U.S.A. 76, 4061–4065 (1979).

    Article  ADS  CAS  Google Scholar 

  2. Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. J. Immun. 234, 2894–2896 (1979).

    Google Scholar 

  3. Meuer, C., Schlossman, S. F. & Reinherz, E. L. Proc. natn. Acad. Sci. U.S.A. 79, 4395–4399 (1982).

    Article  ADS  CAS  Google Scholar 

  4. Biddison, W., Rao, P., Thalle, M., Goldstein, G. & Shaw, S. J. exp. Med. 156, 1065–1076 (1982).

    Article  CAS  Google Scholar 

  5. Krensky, A. M., Reiss, C. S., Mier, J. W., Strominger, J. L. & Burakoff, S. J. Proc. natn. Acad. Sci. U.S.A. 79, 2365–2369 (1982).

    Article  ADS  CAS  Google Scholar 

  6. Gay, D. et al. Nature 328, 626–629 (1987).

    Article  ADS  CAS  Google Scholar 

  7. Engleman, E. C., Benike, C. J., Grumet, C. & Evans, R. L. J. Immun. 127, 2124–2129 (1981).

    CAS  PubMed  Google Scholar 

  8. Doyle, C. & Strominger, J. L. Nature 330, 256–289 (1987).

    Article  ADS  CAS  Google Scholar 

  9. Kruisbeek, A. M. et al. J. exp. Med. 161, 1029–1047 (1985).

    Article  CAS  Google Scholar 

  10. Eichmann, K., Jonsson, J. I., Falk, I. & Emmrich, F. Eur. J. Immun. 17, 643–650 (1987).

    Article  CAS  Google Scholar 

  11. Rudd, C. E., Trevillyan, J. M., Dasgupta, J. D., Wong, L. L. & Schlossman, S. F. Proc. natn. Acad. Sci. U.S.A. 85, 5190–5194 (1988).

    Article  ADS  CAS  Google Scholar 

  12. Veillette, A., Bookman, M. A., Horak, E. M. & Bolen, J. B. Cell 56, 301–308 (1988).

    Article  Google Scholar 

  13. Klatzmann, D. et al. Science 225, 59–63 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Dalgleish, A. et al. Nature 312, 763–766 (1984).

    Article  ADS  CAS  Google Scholar 

  15. Sattentau, Q., Dalgleish, A., Weiss, R. & Beverley, P. C. L. Science 234, 1120–1123 (1986).

    Article  ADS  CAS  Google Scholar 

  16. McDougal, J. S. et al. J. Immun. 137, 2937–2944 (1986).

    CAS  Google Scholar 

  17. McDougal, J. S. et al. Science 231, 382–385 (1986).

    Article  ADS  CAS  Google Scholar 

  18. Maddon, P. et al. Cell 47, 333–348 (1986).

    Article  CAS  Google Scholar 

  19. Sodroski, J., Goh, W. C., Rosen, C., Campbell, K. & Haseltine, W. A. Nature 322, 470–474 (1986).

    Article  ADS  CAS  Google Scholar 

  20. Lifson, J. et al. Nature 323, 725–728 (1986).

    Article  ADS  CAS  Google Scholar 

  21. Maddon, P. et al. Cell 42, 93–104 (1985).

    Article  CAS  Google Scholar 

  22. Clark, S. J. et al. Proc. natn. Acad. Sci. U.S.A. 84, 1649–1653 (1987).

    Article  ADS  CAS  Google Scholar 

  23. Peterson, A. & Seed, B. Cell 54, 65–72 (1988).

    Article  CAS  Google Scholar 

  24. Sattentau, Q. et al. J. exp. Med. 170, 1319–1334 (1989).

    Article  CAS  Google Scholar 

  25. Williams, A. F., Davis, S. J., He, Q. & Barclay, A. N. Cold Spring Harb. Symp. quant. Biol. 54, 637–647 (1989).

    Article  CAS  Google Scholar 

  26. Sayre, P. & Reinherz, E. L. Eur. J. Immun. 15, 291–295 (1985).

    Article  CAS  Google Scholar 

  27. Smith, D. H. et al. Science 238, 1704–1707 (1987).

    Article  ADS  CAS  Google Scholar 

  28. Fisher, R. A. et al. Nature 331, 76–78 (1988).

    Article  ADS  CAS  Google Scholar 

  29. Hussey, R. E. et al. Nature 331, 78–81 (1988).

    Article  ADS  CAS  Google Scholar 

  30. Deen, K. C. et al. Nature 331, 82–84 (1988).

    Article  ADS  CAS  Google Scholar 

  31. Traunecker, A., Luke, W. & Karjalainen, K. Nature 331, 84–86 (1988).

    Article  ADS  CAS  Google Scholar 

  32. Berger, E. A., Fuerst, T. R. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 85, 2357–2361 (1988).

    Article  ADS  CAS  Google Scholar 

  33. Richardson, N. E. et al. Proc. natn. Acad. Sci. U.S.A. 85, 6102–6106 (1988).

    Article  ADS  CAS  Google Scholar 

  34. Clayton, L. K. et al. Nature 335, 363–366 (1988).

    Article  ADS  CAS  Google Scholar 

  35. Mizukami, T., Fuerst, T. R., Berger, E. A. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 85, 9273–9277 (1988).

    Article  ADS  CAS  Google Scholar 

  36. Arthos, J. et al. Cell 57, 469–481 (1989).

    Article  CAS  Google Scholar 

  37. Landau, N. R., Warton, M. & Littman, D. R. Nature 334, 159–162 (1988).

    Article  ADS  CAS  Google Scholar 

  38. Clayton, L. K., Sieh, M., Pious, D. A. & Reinherz, E. L. Nature 339, 548–551 (1989).

    Article  ADS  CAS  Google Scholar 

  39. Lamarre, D. et al. Science 245, 743–746 (1989).

    Article  ADS  CAS  Google Scholar 

  40. Davis, S. J. et al. J. molec. Biol. 213, 7–10 (1990).

    Article  CAS  Google Scholar 

  41. Kwong, P. D. et al. Proc. natn. Acad. Sci. U.S.A. 87, 6423–6427 (1990).

    Article  ADS  CAS  Google Scholar 

  42. Harrison, S. C. et al. manuscript in preparation.

  43. Ryu, S.-E. et al. Nature 348, 419–426 (1990).

    Article  ADS  CAS  Google Scholar 

  44. Garlick, R. L. et al. AIDS Res. hum. Retrovirus 6, 465–479 (1990).

    Article  CAS  Google Scholar 

  45. Wang, B. C. Meth. Enzym. 115, 90–111 (1985).

    Article  CAS  Google Scholar 

  46. Bricogne, G. Acta crystallogr. A 32, 832–847 (1976).

    Article  Google Scholar 

  47. Jones, T. A. J. Appl. crystallogr. 11, 268–272 (1978).

    Article  CAS  Google Scholar 

  48. Brünger, A., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).

    Article  ADS  Google Scholar 

  49. Brünger, A. T. XPLOR Manual, Version 2.1. (Yale University, New Haven, Connecticut, USA).

  50. Stuart, D. & Artymiuk, P. Acta crystallogr. A 40, 713–716 (1985).

    Article  Google Scholar 

  51. Crowther, R. A. in The Molecular Replacement Method 173–183 (ed. Rossmann, M. G.) (Gordon and Breach, New York, 1972).

    Google Scholar 

  52. Huber, R. in Molecular Replacement. Proceedings of the Daresbury Study Weekend, 58–61 (SERC, Daresbury, UK, 1985).

    Google Scholar 

  53. Sussman, J. L., Holbrook, S. R., Church, G. M. & King, S. H. Acta crystallogr. 33, 800–804 (1977).

    Article  Google Scholar 

  54. Bjorkman, P. J. et al. Nature 329, 506–511 (1987).

    Article  ADS  CAS  Google Scholar 

  55. Epp, O. et al. Biochemistry 14, 4943–4952 (1975).

    Article  CAS  Google Scholar 

  56. Lesk, A. M. & Chothia, C. J. molec. Biol. 160, 325–342 (1982).

    Article  CAS  Google Scholar 

  57. Colman, P. M. Adv. Immun. 43, 99–132 (1988).

    Article  CAS  Google Scholar 

  58. Kabat, E. et al. Sequences of Proteins of Immunological Interest (US Dept. of Health & Human Services, Bethesda, Maryland, 1987).

    Google Scholar 

  59. Hunkapiller, T. & Hood, L. Adv. Immun. 44, 1–63 (1989).

    Article  CAS  Google Scholar 

  60. Tramontano, A., Chothia, C. & Lesk, A. M. J. molec. Biol. 215, 175–182 (1990).

    Article  CAS  Google Scholar 

  61. Richardson, J. & Richardson, D. C. in Prediction of Protein Structure and the Principles of Protein Conformation (ed. Fasman, G.) (Plenum, New York, 1989).

    Google Scholar 

  62. Kirszbaum, L. et al. J. Immun. 142, 3931–3936 (1989).

    CAS  PubMed  Google Scholar 

  63. Holmgren, A. & Bränden, C. I. Nature 342, 248–251 (1989).

    Article  ADS  CAS  Google Scholar 

  64. Brodsky, M. H., Warton, M., Myers, R. M. & Littman, D. R. J. Immun. 144, 3078–3086 (1990).

    CAS  PubMed  Google Scholar 

  65. Ashkenazi, A. et al. Proc. natn. Acad. Sci. U.S.A. 87, 7150–7154 (1990).

    Article  ADS  CAS  Google Scholar 

  66. Blum, M., Metcalf, P., Harrison, S. C. & Wiley, D. C. J. appl. Crystallogr. 20, 235–242 (1987).

    Article  CAS  Google Scholar 

  67. Terwilliger, T. C. & Eisenberg, D. Acta crystallogr. A 39, 813–817 (1983).

    Article  Google Scholar 

  68. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    Jiahuai Wang, Youwei Yan, Thomas P. J. Garrett, Jinhuan Liu, David W. Rodgers & Stephen C. Harrison

  2. Howard Hughes Medical Institute, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    Youwei Yan, Thomas P. J. Garrett & Stephen C. Harrison

  3. Upjohn Company, Kalamazoo, Minnesota, 49007, USA

    Robert L. Garlick

  4. Laboratory of Immunobiology, Dana Farber Cancer Institute,

    George E. Tarr, Yasmin Husain & Ellis L. Reinherz

  5. Department of Medicine, Harvard Medical School, 44 Binney Street, Boston, Massachusetts, 02115, USA

    Ellis L. Reinherz

Authors
  1. Jiahuai Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Youwei Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thomas P. J. Garrett
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jinhuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. David W. Rodgers
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Robert L. Garlick
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. George E. Tarr
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yasmin Husain
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ellis L. Reinherz
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Stephen C. Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Yan, Y., Garrett, T. et al. Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domains. Nature 348, 411–418 (1990). https://doi.org/10.1038/348411a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/348411a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing