Abstract
Using an approach for isolating and characterizing peptide fractions that are intracellularly associated with major histocompatibility complex class I molecules, the major peptide recognized by cytotoxic T cells specific for the vesicular stomatitis virus has been isolated from the H–2Kb molecule of infected cells. This endogenously processed octapeptide is allele-specific as it does not bind to H–2Db molecules, and contains the core sequence of the epitope of the nucleocapsid protein of the vesicular stomatitis virus identified by testing with exogenous synthetic peptides.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Zinkernagel, R. M. & Doherty, P. C. Adv. Immun. 27, 51–177 (1979).
Townsend, A. R. M., Gotch, F. M. & Davey, J. Cell 42, 457–467 (1985).
Townsend, A. R. M. et al. Nature 340, 443–448 (1989).
Cox, J. H., Yewdell, J. W., Eisenlohr, L. C., Johnson, P. R. & Bennink, J. R. Science 247, 715–718 (1990).
Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).
Nuchtern, J. G., Bonifacino, J., Biddison, W. E. & Klausner, R. Nature 339, 223–226 (1989).
Lie, W.-R. et al. Nature 344, 439–441 (1990).
Hosken, N. A. & Bevan, M. J. Science 248, 367–370 (1990).
Cerundolo, V. et al. Nature 345, 499–452 (1990).
Bastin, J., Rothbard, J. B., Davey, J., Jones, I. & Townsend, A. R. M. J. exp. Med 165, 1508–1523 (1987).
Braciale, T. J. et al. J. exp. Med. 166, 678–692 (1987).
Oldstone, M. B. A., Whitton, J. L., Lewicki, H. & Tishon, A. J. exp. Med 168, 559–570 (1988).
Bjorkman, P. J. et al. Nature 329, 506–512 (1987).
Bjorkman, P. J. et al. Nature 329, 512–518 (1987).
Garrett, T. P. J., Saper, M. A., Bjorkman, P. J., Strominger, J. L. & Wiley, D. C. Nature 342, 692–696 (1989).
Shimojo, N., Maloy, W. L., Anderson, R. W., Biddison, W. E. & Coligan, J. E. J. Immun. 143, 2939–2947 (1989).
Takahashi, H. et al. J. exp. Med. 170, 2023–2035 (1989).
Maryanski, J. L., Verdini, A. S., Weber, P. C., Salemme, F. R. & Corradin, G. Cell 60, 63–72 (1990).
DeLisi, C. & Berzofsky, J. A. Proc. Natn. Acad. Sci. U.S.A. 82, 7048–7052 (1985).
Rothbard, J. B. & Taylor, W. R. EMB0 J. 7, 93–100 (1988).
Guillet, J.-G., Lai, M.-Z., Briner, T. J., Smith, J. A. & Gefter, M. L. Nature 324, 260–262 (1986).
Sette, A. et al. Nature 328, 395–399 (1987).
Ogasawara, K., Maloy, W. L., Beverly, B. & Schwartz, R. H. J. Immun. 142, 1448–1456 (1989).
Bhayani, H. & Paterson, Y. J. exp. Med 170, 1609–1625 (1989).
Sette, A., Buus, S., Colon, S. M., Miles, C. & Grey, H. M. J. Immun. 141, 45–54 (1988).
Sette, A. et al. J. Immun. 143, 3289–3294 (1989).
Fox, B. S., Carbone, F. R., Germain, R. N., Paterson, Y. & Schwartz, R. H. Nature 331, 538–540 (1988).
Bodmer, H. C., Gotch, F. M. & McMichael, A. J. Nature 337, 653–655 (1989).
Puddington, L., Bevan, M. J., Rose, J. K. & Lefrancois, L. J. Virol. 60, 708–717 (1986).
Sheil, J. M., Bevan, M. J. & Lefrancois, L. J. Immun. 138, 3654–3660 (1987).
Van der Zee, R., Van Eden, W., Meloen, R. H., Noordzij, A. & Van Embden, J. D. A. Eur. J. Immun. 19, 43–47 (1989).
Gallione, C. J., Greene, J. R., Iverson, L. E. & Rose, J. K. J. Virol. 39, 529–535 (1981).
Taylor, P. M., Davey, J., Howland, K., Rothbard, J. B. & Askonas, B. A. Immunogenetics 26, 267–272 (1987).
Bodmer, H. C., Pemberton, R. M., Rothbard, J. B. & Askonas, B. A. Cell 52, 253–258 (1988).
Kast, W. M. et al. Cell 59, 603–614 (1989).
Demotz, S., Grey, H. M., Appella, E. & Sette, A. Nature 342, 682–684 (1989).
Bouillot, M. et al. Nature 339, 473–475 (1989).
Carreno, B. M., Anderson, R. W., Coligan, J. E. & Biddison, W. E. Proc. natn. Acad. Sci. U.S.A. 87, 3420–3424 (1990).
Schild, H., Rotzschke, O., Kalbacher, H. & Rammensee, H.-G. Science 247, 1587–1589 (1990).
Nikolic–Zugic, J. & Bevan, M. J. Nature 344, 65–67 (1990).
Geysen, H. M., Meloen, R. H. & Barteling, S. J. Proc. natn. Acad. Sci. U.S.A. 81, 3998–4002 (1984).
Geysen, H. M., Barteling, S. J. & Meloen, R. H. Proc. natn Acad. Sci. U.S.A. 82, 178–182 (1985).
DeWaal, L. P., Kast, W. M., Melvold, R. W. & Melief, C. J. M. J. Immun. 130, 1090–1096 (1983).
Kohler, G., Fischer-Lindahl, K. & Heusser, C. in The Immune System, 202–208 (Karger, Basel, 1981).
Jones, B. & Janeway, C. A. Nature 292, 547–549 (1981).
Rotzschke, O., Falk, K., Wallny, H.-J., Faath, S. & Rammensee, H.-G. Science 249, 283–286 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bleek, G., Nathenson, S. Isolation of an endogenously processed immunodominant viral peptide from the class I H–2Kb molecule. Nature 348, 213–216 (1990). https://doi.org/10.1038/348213a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/348213a0
This article is cited by
-
Virus-based vaccine vectors with distinct replication mechanisms differentially infect and activate dendritic cells
npj Vaccines (2021)
-
Mass spectrometry–based identification of MHC-bound peptides for immunopeptidomics
Nature Protocols (2019)
-
The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer
British Journal of Cancer (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.