Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Isolation of an endogenously processed immunodominant viral peptide from the class I H–2Kb molecule

Abstract

Using an approach for isolating and characterizing peptide fractions that are intracellularly associated with major histocompatibility complex class I molecules, the major peptide recognized by cytotoxic T cells specific for the vesicular stomatitis virus has been isolated from the H–2Kb molecule of infected cells. This endogenously processed octapeptide is allele-specific as it does not bind to H–2Db molecules, and contains the core sequence of the epitope of the nucleocapsid protein of the vesicular stomatitis virus identified by testing with exogenous synthetic peptides.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Zinkernagel, R. M. & Doherty, P. C. Adv. Immun. 27, 51–177 (1979).

    Article  CAS  Google Scholar 

  2. Townsend, A. R. M., Gotch, F. M. & Davey, J. Cell 42, 457–467 (1985).

    Article  CAS  Google Scholar 

  3. Townsend, A. R. M. et al. Nature 340, 443–448 (1989).

    Article  ADS  CAS  Google Scholar 

  4. Cox, J. H., Yewdell, J. W., Eisenlohr, L. C., Johnson, P. R. & Bennink, J. R. Science 247, 715–718 (1990).

    Article  ADS  CAS  Google Scholar 

  5. Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).

    Article  ADS  CAS  Google Scholar 

  6. Nuchtern, J. G., Bonifacino, J., Biddison, W. E. & Klausner, R. Nature 339, 223–226 (1989).

    Article  ADS  CAS  Google Scholar 

  7. Lie, W.-R. et al. Nature 344, 439–441 (1990).

    Article  ADS  CAS  Google Scholar 

  8. Hosken, N. A. & Bevan, M. J. Science 248, 367–370 (1990).

    Article  ADS  CAS  Google Scholar 

  9. Cerundolo, V. et al. Nature 345, 499–452 (1990).

    Article  Google Scholar 

  10. Bastin, J., Rothbard, J. B., Davey, J., Jones, I. & Townsend, A. R. M. J. exp. Med 165, 1508–1523 (1987).

    Article  CAS  Google Scholar 

  11. Braciale, T. J. et al. J. exp. Med. 166, 678–692 (1987).

    Article  CAS  Google Scholar 

  12. Oldstone, M. B. A., Whitton, J. L., Lewicki, H. & Tishon, A. J. exp. Med 168, 559–570 (1988).

    Article  CAS  Google Scholar 

  13. Bjorkman, P. J. et al. Nature 329, 506–512 (1987).

    Article  ADS  CAS  Google Scholar 

  14. Bjorkman, P. J. et al. Nature 329, 512–518 (1987).

    Article  ADS  CAS  Google Scholar 

  15. Garrett, T. P. J., Saper, M. A., Bjorkman, P. J., Strominger, J. L. & Wiley, D. C. Nature 342, 692–696 (1989).

    Article  ADS  CAS  Google Scholar 

  16. Shimojo, N., Maloy, W. L., Anderson, R. W., Biddison, W. E. & Coligan, J. E. J. Immun. 143, 2939–2947 (1989).

    CAS  PubMed  Google Scholar 

  17. Takahashi, H. et al. J. exp. Med. 170, 2023–2035 (1989).

    Article  CAS  Google Scholar 

  18. Maryanski, J. L., Verdini, A. S., Weber, P. C., Salemme, F. R. & Corradin, G. Cell 60, 63–72 (1990).

    Article  CAS  Google Scholar 

  19. DeLisi, C. & Berzofsky, J. A. Proc. Natn. Acad. Sci. U.S.A. 82, 7048–7052 (1985).

    Article  ADS  CAS  Google Scholar 

  20. Rothbard, J. B. & Taylor, W. R. EMB0 J. 7, 93–100 (1988).

    Article  CAS  Google Scholar 

  21. Guillet, J.-G., Lai, M.-Z., Briner, T. J., Smith, J. A. & Gefter, M. L. Nature 324, 260–262 (1986).

    Article  ADS  CAS  Google Scholar 

  22. Sette, A. et al. Nature 328, 395–399 (1987).

    Article  ADS  CAS  Google Scholar 

  23. Ogasawara, K., Maloy, W. L., Beverly, B. & Schwartz, R. H. J. Immun. 142, 1448–1456 (1989).

    CAS  PubMed  Google Scholar 

  24. Bhayani, H. & Paterson, Y. J. exp. Med 170, 1609–1625 (1989).

    Article  CAS  Google Scholar 

  25. Sette, A., Buus, S., Colon, S. M., Miles, C. & Grey, H. M. J. Immun. 141, 45–54 (1988).

    CAS  PubMed  Google Scholar 

  26. Sette, A. et al. J. Immun. 143, 3289–3294 (1989).

    CAS  Google Scholar 

  27. Fox, B. S., Carbone, F. R., Germain, R. N., Paterson, Y. & Schwartz, R. H. Nature 331, 538–540 (1988).

    Article  ADS  CAS  Google Scholar 

  28. Bodmer, H. C., Gotch, F. M. & McMichael, A. J. Nature 337, 653–655 (1989).

    Article  ADS  CAS  Google Scholar 

  29. Puddington, L., Bevan, M. J., Rose, J. K. & Lefrancois, L. J. Virol. 60, 708–717 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Sheil, J. M., Bevan, M. J. & Lefrancois, L. J. Immun. 138, 3654–3660 (1987).

    CAS  PubMed  Google Scholar 

  31. Van der Zee, R., Van Eden, W., Meloen, R. H., Noordzij, A. & Van Embden, J. D. A. Eur. J. Immun. 19, 43–47 (1989).

    Article  CAS  Google Scholar 

  32. Gallione, C. J., Greene, J. R., Iverson, L. E. & Rose, J. K. J. Virol. 39, 529–535 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Taylor, P. M., Davey, J., Howland, K., Rothbard, J. B. & Askonas, B. A. Immunogenetics 26, 267–272 (1987).

    Article  CAS  Google Scholar 

  34. Bodmer, H. C., Pemberton, R. M., Rothbard, J. B. & Askonas, B. A. Cell 52, 253–258 (1988).

    Article  CAS  Google Scholar 

  35. Kast, W. M. et al. Cell 59, 603–614 (1989).

    Article  CAS  Google Scholar 

  36. Demotz, S., Grey, H. M., Appella, E. & Sette, A. Nature 342, 682–684 (1989).

    Article  ADS  CAS  Google Scholar 

  37. Bouillot, M. et al. Nature 339, 473–475 (1989).

    Article  ADS  CAS  Google Scholar 

  38. Carreno, B. M., Anderson, R. W., Coligan, J. E. & Biddison, W. E. Proc. natn. Acad. Sci. U.S.A. 87, 3420–3424 (1990).

    Article  ADS  CAS  Google Scholar 

  39. Schild, H., Rotzschke, O., Kalbacher, H. & Rammensee, H.-G. Science 247, 1587–1589 (1990).

    Article  ADS  CAS  Google Scholar 

  40. Nikolic–Zugic, J. & Bevan, M. J. Nature 344, 65–67 (1990).

    Article  ADS  Google Scholar 

  41. Geysen, H. M., Meloen, R. H. & Barteling, S. J. Proc. natn. Acad. Sci. U.S.A. 81, 3998–4002 (1984).

    Article  ADS  CAS  Google Scholar 

  42. Geysen, H. M., Barteling, S. J. & Meloen, R. H. Proc. natn Acad. Sci. U.S.A. 82, 178–182 (1985).

    Article  ADS  CAS  Google Scholar 

  43. DeWaal, L. P., Kast, W. M., Melvold, R. W. & Melief, C. J. M. J. Immun. 130, 1090–1096 (1983).

    CAS  Google Scholar 

  44. Kohler, G., Fischer-Lindahl, K. & Heusser, C. in The Immune System, 202–208 (Karger, Basel, 1981).

    Google Scholar 

  45. Jones, B. & Janeway, C. A. Nature 292, 547–549 (1981).

    Article  ADS  CAS  Google Scholar 

  46. Rotzschke, O., Falk, K., Wallny, H.-J., Faath, S. & Rammensee, H.-G. Science 249, 283–286 (1990).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bleek, G., Nathenson, S. Isolation of an endogenously processed immunodominant viral peptide from the class I H–2Kb molecule. Nature 348, 213–216 (1990). https://doi.org/10.1038/348213a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/348213a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing