A PRODUCT encoded at the claret locus in Drosophila is needed for normal chromosome segregation in meiosis in females and in early mitotic divisions of the embryo1,2. The predicted amino-acid sequence of the segregation protein was shown recently to be strikingly similar to Drosophila kinesin heavy chain3. We have expressed the claret segregation protein in bacteria and have found that the bacterially expressed protein has motor activity in vitro with several novel features. The claret motor is slow (4 μm min−1), unlike either kinesin or dyneins. It has the directionality, the ability to generate torque and the sensitivity to inhibitors reported previously for dyneins. The finding of minus-end directed motor activity for a protein with sequence similarity to kinesin suggests that the dynein and kinesin motor domains are ancestrally related. The minus-end directed motor activity of the claret motor is consistent with a role for this protein in producing chromosome movement along spindle microtubules during prometaphase and/or anaphase.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Sturtevant, A. H. Z. Wiss. Zool. 135, 323–356 (1929).
Lewis, E. B. & Gencarella, W. Genetics 37, 600–601 (1952).
Endow, S. A., Henikoff, S. & Niedziela, L. S. Nature 345, 81–83 (1990).
Yamamoto, A. H., Komma, D. J., Shaffer, C. D., Pirrotta, V. & Endow, S. A. EMBO J. 8, 3543–3552 (1989).
McDonald, H. B. & Goldstein, L. S. B. Cell 61, 991–1000 (1990).
Walker, R. A., Gliksman, N. R. & Salmon, E. D. in Optical Microscopy for Biology (eds Herman, B. & Jacobsen, K.) 395–407 (Wiley-Liss, New York, 1990).
Yang, J. T., Saxton, W. M., Stewart, R. J., Raff, E. G. & Goldstein, L. S. B. Science 249, 42–47 (1990).
Mcintosh, J. R. & Porter, M. E. J. biol. Chem. 284, 6001–6004 (1989).
Paschal, B. M. & Vallee, R. B. Nature 330, 181–183 (1987).
Cohn, S. A., Ingold, A. L. & Scholey, J. M. J. biol. Chem. 264, 4290–4297 (1989).
Vale, R. D., Schnapp, B. J., Reese, T. S. & Sheetz, M. P. Cell 40, 559–569 (1985).
Paschal, B. M., Shpetner, H. S. & Vallee, R. B. J. Cell Biol. 105, 1273–1282 (1987).
Paschal, B. M. et al. Nature 330, 672–674 (1987).
Vale, R. D. & Toyoshima, Y. Y. Cell 52, 459–469 (1988).
Belmont, L. D., Hyman, A. A., Sawin, K. E. & Mitchison, T. J. Cell 62, 579–589 (1990).
Hyman, A. A. et al. Meth. Enzym. (in the press).
Vale, R. D. et al. Cell 43, 623–632 (1985).
Vale, R. D. & Goldstein, L. S. B. Cell 60, 883–885 (1990).
Salmon, E. D. in Mitosis (eds Hyams, J. S. & Brinkley, B. R.) 119–181 (Academic, San Diego, 1989).
Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. Meth. Enzym. 185, 60–89 (1990).
Walker, R. A. et al. J. Cell Biol. 107, 1437–1448 (1988).
Koshland, D. E., Mitchison, T. J. & Kirschner, M. W. Nature 331, 499–504 (1988).
Sale, W. S. & Fox, L. A. J. Cell Biol. 107, 1793–1797 (1988).
Kuznetsov, S. A. et al. EMBO J. 7, 353–358 (1988).
About this article
Cite this article
Walker, R., Salmon, E. & Endow, S. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature 347, 780–782 (1990). https://doi.org/10.1038/347780a0
Developmental Cell (2021)
Current Biology (2020)
Journal of Biological Chemistry (2020)
N‐terminal β‐strand of single‐headed kinesin‐1 can modulate the off‐axis force‐generation and resultant rotation pitch
Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other
Nature Communications (2020)