Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Atomic structure of the actin: DNase I complex

Nature volume 347, pages 37–44 (1990)Cite this article

Abstract

The atomic models of the complex between rabbit skeletal muscle actin and bovine pancreatic deoxyribonuclease I both in the ATP and ADP forms have been determined byo X-ray analysis at an effective resolution of 2.8 Å and 3 Å, respectively. The two structures are very similar. The actin molecule consists of two domains which can be further subdivided into two subdomains. ADP or ATP is located in the cleft between the domains with a calcium ion bound to the β- or β- and γ-phosphates, respectively. The motif of a five-stranded (3 sheet consisting of a (3 meander and a right handed β α β unit appears in each domain suggesting that gene duplication might have occurred. These sheets have the same topology as that found in hexokinase.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

References

  1. Straub, F. B. Studies, University of Szeged II, 3–15 (1942).

    Google Scholar 

  2. Straub, F. B. Studies, University of Szeged III, 23–27 (1943).

    Google Scholar 

  3. Pollard, T. D. Curr. Opinion Cell Biol. 2, 33–40 (1990).

    CAS  Article  Google Scholar 

  4. Vandekerckhove, J. Curr. Opinion Cell Biol. 2, 41–50 (1990).

    CAS  Article  Google Scholar 

  5. Elzinga, M., Collins, J. H., Kuehl, W. M. & Adelstein, R. S. Proc. natn. Acad. Sci. U.S.A. 70, 2687–2691 (1973).

    ADS  CAS  Article  Google Scholar 

  6. Collins, J. H. & Elzinga, M. J. biol. Chem. 250, 5915–5920 (1975).

    CAS  PubMed  Google Scholar 

  7. Lu, R. C. & Elzinga, M. Biochemistry 16, 5801–5806 (1977).

    CAS  Article  Google Scholar 

  8. Vandekerckhove, J. & Weber, K. Eur. J. Biochem. 90, 451–462 (1978).

    CAS  Article  Google Scholar 

  9. Lazarides, E. & Lindberg, U. Proc. natn. Acad. Sci. U.S.A. 71, 4742–4746 (1974).

    ADS  CAS  Article  Google Scholar 

  10. Mannherz, H. G., Barrington-Leigh, J., Leberman, R. & Pfrang, H. FEBS Lett. 60, 34–38 (1975).

    CAS  Article  Google Scholar 

  11. Hitchcock, S. E. J. biol. Chem. 255, 5668–5673 (1980).

    CAS  PubMed  Google Scholar 

  12. Mannherz, H. G., Kabsch, W. & Leberman, R. FEBS Lett. 73, 141–143 (1977).

    CAS  Article  Google Scholar 

  13. Sugino H. et al. J. Biochem. 86, 257–260 (1979).

    CAS  PubMed  Google Scholar 

  14. Suck, D., Kabsch, W. & Mannherz, H. G. Proc. natn. Acad. Sci. U.S.A. 78, 4319–4323 (1981).

    ADS  CAS  Article  Google Scholar 

  15. Kabsch, W., Mannherz, H. G. & Suck, D. EMBO J. 4, 2113–2118 (1985).

    CAS  Article  Google Scholar 

  16. Suck, D., Oefner, C. & Kabsch, W. EMBO J. 3, 2423–2430 (1984).

    CAS  Article  Google Scholar 

  17. Oefner, C. & Suck, D. J. molec. Biol. 192, 605–632 (1986).

    CAS  Article  Google Scholar 

  18. Kabsch, W. J. appl. Crystallogr. 21, 916–924 (1988).

    CAS  Article  Google Scholar 

  19. Kabsch, W. J. appl. Crystallogr. 21, 67–71 (1988).

    CAS  Article  Google Scholar 

  20. Kabsch, W., Pai, E. F., Mannherz, H. G. & Suck, D. Springer Series in Biophysics (eds Aebi U. & Engel, J.) 3, 42–47 (1989).

    Google Scholar 

  21. Bruenger, A. T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).

    ADS  CAS  Article  Google Scholar 

  22. Read, R. J. Acta crystallogr. A42, 140–149 (1986).

    CAS  Article  Google Scholar 

  23. Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).

    CAS  Article  Google Scholar 

  24. Steitz, T. A., Fletterick, R. J., Anderson, W. A. & Anderson, C. M. J. molec. Biol. 104, 197–222 (1976).

    CAS  Article  Google Scholar 

  25. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    CAS  Article  Google Scholar 

  26. Naharro, G., Robbins, K. C. & Reddy, E. P. Science 223, 63–66 (1984).

    ADS  CAS  Article  Google Scholar 

  27. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W., Nature 347, 44–49 (1990).

    ADS  CAS  Article  Google Scholar 

  28. Polzar, B., Nowak, E., Goody, R. S. & Mannherz, H. G. Eur. J. Biochem. 182, 267–275 (1989).

    CAS  Article  Google Scholar 

  29. Suck, D., Lahm, A. & Oefner, C. Nature 332, 464–468 (1988).

    ADS  CAS  Article  Google Scholar 

  30. Boyer, M., Roustan, C. & Benyamin, Y. Biosci. Rpt 5, 39–46 (1985).

    CAS  Article  Google Scholar 

  31. Sutoh, K. Biochemistry 23, 1942–1946 (1984).

    CAS  Article  Google Scholar 

  32. Mornet, D. & Ue, K. Proc. natn. Acad. Sci. U.S.A. 81, 3680–3684 (1984).

    ADS  CAS  Article  Google Scholar 

  33. Mornet, D., Bonet, A., Audemard, E. & Bonicel, J. J. Muscle Res. Cell Motil. 10, 10–24 (1989).

    CAS  Article  Google Scholar 

  34. Jacobson, G. R. & Rosenbusch, J. P. Proc. natn. Acad. Sci. U.S.A. 73, 2742–2746 (1976).

    ADS  CAS  Article  Google Scholar 

  35. Johnson, P., Wester, P. J. & Hikida, R. S. Biochim. biophys. Acta 578, 253–257 (1979).

    CAS  Article  Google Scholar 

  36. Konno, K. J. Biochem., Tokyo 103, 386–392 (1988).

    CAS  Article  Google Scholar 

  37. Schwyter, D., Phillips, M. & Reisler, E. Biochemistry 28, 5889–5895 (1989).

    CAS  Article  Google Scholar 

  38. Carlsson, L. et al. J. molec. Biol. 105, 353–366 (1976).

    CAS  Article  Google Scholar 

  39. Schutt, C. E., Lindberg, U., Myslik, J. & Strauss, N. J. molec. Biol. 209, 735–746 (1989).

    CAS  Article  Google Scholar 

  40. Vandekerckhove, J. S., Kaiser, D. A. & Pollard, T. D. J. Cell Biol. 109, 619–626 (1989).

    CAS  Article  Google Scholar 

  41. Kuwayama, H. & Yount, R. G. Biophys. J. 49, 454a (1986).

    Google Scholar 

  42. Hegyi, Szilagyi, L. & Elzinga, M. Biochemistry 25, 5793–5798 (1986).

    CAS  Article  Google Scholar 

  43. Saenger, W. Principles of Nucleic Acid Structure 74 (Springer-Verlag, 1984).

    Book  Google Scholar 

  44. DePhamphilis, M. L. & Cleland, W. W. Biochemistry 12, 3714–3723 (1973).

    Article  Google Scholar 

  45. Strzelecka-Golaszewska, H., Boguta, G., Zmorzynski, S. & Moraczewska, J. Eur. J. Biochem. 182, 299–305 (1989).

    CAS  Article  Google Scholar 

  46. Nowak, E., Strzelecka-Golaszewska, H. & Goody, R. S. Biochemistry 27, 1785–1792 (1988).

    CAS  Article  Google Scholar 

  47. Loscalzo, J. & Reed, G. H. Biochemistry, 15, 5407–5412 (1976).

    CAS  Article  Google Scholar 

  48. Brauer, M. & Sykes, B. D. Biochemistry 21, 5934–5939 (1982).

    CAS  Article  Google Scholar 

  49. Miki, M. & Wahl, P. Biochim. biophys. Acta 828, 188–195 (1985).

    CAS  Article  Google Scholar 

  50. Valentin-Ranc, C. & Carlier, M.-F. J. biol. Chem. 264, 20871–20880 (1989).

    CAS  PubMed  Google Scholar 

  51. Moore, P. B., Huxley, H. E. & DeRosier, D. J. J. molec. Biol. 50, 279–295 (1970).

    CAS  Article  Google Scholar 

  52. Sutoh, K. Biochemistry 21, 3654–3661 (1982).

    CAS  Article  Google Scholar 

  53. Bertrand, R. et al. Biochemistry 27, 5728–5736 (1988).

    CAS  Article  Google Scholar 

  54. Méjean, C. et al. Biosci. Rept 6, 493–499 (1986).

    Article  Google Scholar 

  55. Miller, L., Kalnowski, M., Yunossi, Z., Bulsinki, J. C. & Reisler, E. Biochemistry 26, 6064–6070 (1987).

    CAS  Article  Google Scholar 

  56. Moir, A. J. G., Levine, B. A., Goodearl, A. J. & Trayer, I. P. J. Muscle Res. Cell Motil. 8, 68–69 (1988).

    Google Scholar 

  57. Barden, J. A., Phillips, L., Cornell, B. A. & dos Remedios, C. G. Biochemistry 28, 5895–5901 (1989).

    CAS  Article  Google Scholar 

  58. Barden, J. A. & Phillips, L. Biochemistry 29, 1348–1354 (1990).

    CAS  Article  Google Scholar 

  59. Szilagyi, L. & Lu, R. C. Biochim. Biophys. Acta 709, 204–211 (1982).

    CAS  Article  Google Scholar 

  60. Trayer, I. P., Trayer, H. R. & Levine, B. A. Eur. J. Biochem. 164, 259–266 (1987).

    CAS  Article  Google Scholar 

  61. Flaherty, K. M., DeLuca-Flaherty, C. & McKay, D. B. Nature 346, 623–628 (1990).

    ADS  CAS  Article  Google Scholar 

  62. Blum, M., Metcalf, P. & Wiley, D. C. J. appl. Crystallogr. 20, 235–247 (1987).

    CAS  Article  Google Scholar 

  63. dos Remedios, C., Miki, M. & Barden, J. A. J. Muscle Res. Cell Motil. 8, 97–117 (1987).

    CAS  Article  Google Scholar 

  64. Priestle, J. P. J. appl. Crystallogr. 21, 572–576 (1988).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Hans Georg Mannherz: Institut für Anatomie und Zellbiologie der Philipps-Universitat, Robert-Koch-StraBe 6, 3550 Marburg, FRG

  2. Dietrich Suck: European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg, FRG

Affiliations

  1. Max-Planck-lnstitut für medizinische Forschung, Abteilung Biophysik, JahnstraBe 29, 6900, Heidelberg, FRG

    Wolfgang Kabsch, Hans Georg Mannherz, Dietrich Suck, Emil F. Pai & Kenneth C. Holmes

Authors
  1. Wolfgang Kabsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hans Georg Mannherz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dietrich Suck
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emil F. Pai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kenneth C. Holmes
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kabsch, W., Mannherz, H., Suck, D. et al. Atomic structure of the actin: DNase I complex . Nature 347, 37–44 (1990). https://doi.org/10.1038/347037a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/347037a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing