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Atomic structure of the actin: DNase I complex

Nature volume 347, pages 37–44 (1990)Cite this article

Abstract

The atomic models of the complex between rabbit skeletal muscle actin and bovine pancreatic deoxyribonuclease I both in the ATP and ADP forms have been determined byo X-ray analysis at an effective resolution of 2.8 Å and 3 Å, respectively. The two structures are very similar. The actin molecule consists of two domains which can be further subdivided into two subdomains. ADP or ATP is located in the cleft between the domains with a calcium ion bound to the β- or β- and γ-phosphates, respectively. The motif of a five-stranded (3 sheet consisting of a (3 meander and a right handed β α β unit appears in each domain suggesting that gene duplication might have occurred. These sheets have the same topology as that found in hexokinase.

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Author notes

  1. Hans Georg Mannherz: Institut für Anatomie und Zellbiologie der Philipps-Universitat, Robert-Koch-StraBe 6, 3550 Marburg, FRG

  2. Dietrich Suck: European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg, FRG

Authors and Affiliations

  1. Max-Planck-lnstitut für medizinische Forschung, Abteilung Biophysik, JahnstraBe 29, 6900, Heidelberg, FRG

    Wolfgang Kabsch, Hans Georg Mannherz, Dietrich Suck, Emil F. Pai & Kenneth C. Holmes

Authors
  1. Wolfgang Kabsch
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  2. Hans Georg Mannherz
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  4. Emil F. Pai
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  5. Kenneth C. Holmes
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Kabsch, W., Mannherz, H., Suck, D. et al. Atomic structure of the actin: DNase I complex . Nature 347, 37–44 (1990). https://doi.org/10.1038/347037a0

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