A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif

Abstract

A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene. This gene is conserved and Y-specific among a wide range of mammals, and encodes a testis-specific transcript. It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. This gene has been termed SRY (for sex-determining region Y) and proposed to be a candidate for the elusive testis-determining gene, TDF.

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References

  1. 1

    Goodfellow, P. N. & Darling, S. M. Development 102, 251–258 (1988).

  2. 2

    Jost, A., Vigier, B., Prepin, J. & Perchellet, J. P. Recent Prog. Horm. Res. 29, 1–41 (1973).

  3. 3

    Vergnaud, G. et al. Am. J. hum. Genet. 38, 109–124 (1986).

  4. 4

    Guellaen, G. et al. Nature 307, 172–173 (1984).

  5. 5

    Goodfellow, P. J., Darling, S. M., Thomas, N. S. & Goodfellow, P. N. Science 234, 740–743 (1986).

  6. 6

    Weissenbach, J., Levilliers, J., Petit, C., Rouyer, F. & Simmler, M.-C. Development 101S, 67–74 (1987).

  7. 7

    Pritchard, C. A., Goodfellow, P. J. & Goodfellow, P. N. Nature 328, 273–275 (1987).

  8. 8

    Ferguson-Smith, M. A. Lancet ii, 475–476 (1966).

  9. 9

    Petit, C. et al. Cell 49, 595–602 (1987).

  10. 10

    Page, D. C. et al. Cell 51, 1091–1104 (1987).

  11. 11

    Palmer, M. S., Berta, P., Sinclair, A. H., Pym, B. & Goodfellow, P. N. Proc. natn. Acad Sci. U.S.A. 87, 1681–1685 (1990).

  12. 12

    Schneider-Gädicke, A., Beer-Romero, P., Brown, L. G., Nussbaum, R. & Page, D. C. Cell 57, 1247–1258 (1989).

  13. 13

    Sinclair, A. H. et al. Nature 336, 780–782 (1988).

  14. 14

    Koopman, P., Gubbay, J., Collignon, J. & Lovell-Badge, R. Nature 342, 940–942 (1989).

  15. 15

    Palmer, M. S. et al. Nature 342, 937–939 (1989).

  16. 16

    Ellis, N. A. et al. Nature 337, 81–84 (1989).

  17. 17

    Sealey, P. G., Whittaker, P. A. & Southern, E. M. Nucleic Acids Res. 13, 1905–1922 (1985).

  18. 18

    Smith, T. F. & Waterman, M. S. J. molec. Biol. 147, 195–197 (1981).

  19. 19

    Collins, J. F., Coulson, A. F. W. & Lyall, A. CABIOS 4, 67–71 (1988).

  20. 20

    Kelly, M., Burke, J., Smith, M., Klar, A. & Beach, D. EMBO J. 7, 1537–1547 (1988).

  21. 21

    Jantzen, H.-M., Adman, A., Bell, S. P. & Tjian, R. Nature 344, 830–836 (1990).

  22. 22

    Kolodrubetz, D. & Burgum, A. J. biol. Chem. 265, 3234–3239 (1990).

  23. 23

    Frohman, M. A., Dush, M. K. & Martin, G. R. Proc. natn. Acad. Sci. U.S.A. 85, 8998–9002 (1988).

  24. 24

    Gubbay, J. et al. Nature 346, 245–250 (1990).

  25. 25

    McLaren, A., Simpson, E., Tomonari, K., Chandler, P. & Hogg, H. Nature 312, 552–555 (1984).

  26. 26

    Lovell-Badge, R. & Robertson, E. Development 109, 635–646 (1990).

  27. 27

    Wright, J. M. & Dixon, G. H. Biochemistry 27, 576–581 (1988).

  28. 28

    Goodfellow, P. J. et al. Ann. hum. Genet 53, 15–22 (1989).

  29. 29

    DeKretser, T. A., Crumpton, M. J., Bodmer, J. G. & Bodmer, W. F. Eur. J. Immun. 12, 600–606 (1982).

  30. 30

    Bishop, C. E. et al. Nature 303, 831–832 (1983).

  31. 31

    Westerveld, A., Visser, R. P. L. S., Meera Khan, P. & Bootsma, D. Nature new Biol. 234, 20–24 (1971).

  32. 32

    Goss, S. J. & Harris, H. Nature 255, 680–683 (1975).

  33. 33

    Burk, R. D., Ma, P. & Smith, K. D. Molec. cell. Biol. 5, 576–581 (1985).

  34. 34

    Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

  35. 35

    Chomczynski, P. & Sacchi, N. Analyt. Biochem. 162, 156–159 (1987).

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Sinclair, A., Berta, P., Palmer, M. et al. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature 346, 240–244 (1990). https://doi.org/10.1038/346240a0

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