Abstract
ALTHOUGH most eukaryotic genes known to be transcribed by RNA polymerase III have intragenic promoter elements, some are similar to genes transcribed by RNA polymerase II in that they have upstream promoters (reviewed in refs 1–4). Transcription of the vertebrate U6 and 7SK RNA genes by RNA polymerase III depends exclusively upon upstream signals, some of which are indistinguishable from the elements essential for polymerase II-specific genes5–11. In the plant Arabidopsis thaliana the promoter elements for the U6 and U2 small nuclear RNA genes, transcribed by RNA polymerases III and II respectively, are identical, comprising a −30 TATA box and an upstream element specific for small nuclear RNA genes12,13. The distance between these elements differs, however. Here we report evidence that this separation is crucial in determining whether the genes are transcribed by polymerase II or III.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Geiduschek, E. P. & Tocchini-Valentini, G. P. A Rev. Biochem. 57, 873–914 (1988).
Sollner-Webb, B. Cell 52, 153–154 (1988).
Murphy, S., Moorefield, B. & Pieler, T. Trends Genet. 5, 122–126 (1989).
Howe, J. G. & Shu, M.-D. Cell 57, 825–834 (1989).
Carbon, P. et al. Cell 51, 71–79 (1987).
Mattaj, I. W., Dathan, N. A., Parry, H. D., Carbon, P. & Krol, A. Cell 55, 435–442 (1988).
Das, G., Henning, D., Wright, D. & Reddy, R. EMBO J. 7, 503–512 (1988).
Kunkel, G. R. & Pederson, T. Genes Dev. 2, 196–204 (1988).
Lobo, S. M. & Hernandez, N. Cell 58, 55–67 (1989).
Murphy, S., Di Liegro, C. & Melli, M. Cell, 51, 81–87 (1987).
Kleinert, H. & Benecke, B.-J. Nucleic Acids Res. 16, 1319–1331 (1988).
Vankan, P. & Filipowicz, W. EMBO J. 8, 3875–3882 (1989).
Waibel, F. & Filipowicz, W. Nucleic Acids Res. 18 (in the press).
Bogenhagen, D. F. & Brown, D. D. Cell 24, 261–270 (1981).
Vankan, P., Edoh, D. & Filipowicz, W. Nucleic Acids Res. 16, 10425–10439 (1988).
Singh, R. & Reddy, R. Proc. natn. Acad. Sci. U.S.A. 86, 8280–8283 (1989).
Mühlbach, H.-P. & Sänger, H. L. Nature 278, 185–188 (1979).
Vankan, P. & Filipowicz, W. EMBO J. 7, 791–799 (1988).
Buratowski, S., Hahn, S., Sharp, P. A. & Guarente, L. Nature 334, 37–42 (1988).
Cavallini, B. et al. Nature 334, 77–80 (1988).
van Santen, V. L., Swain, W. & Spritz, R. A. Nucleic Acids Res. 16, 4176 (1988).
Abel, S., Kiss, T. & Solymosy, F. Nucleic Acids Res. 17, 6319–6337 (1989).
Dahlberg, J. E. & Lund, E. in Structure and Function of Small Nuclear Ribonucleoprotein Particles (ed. M. L. Birnstiel) 38–70 (Springer, Heidelberg, 1988).
Parry, H. D., Scherly, D. & Mattaj, I. W. Trends biochem. Sci. 14, 15–19 (1989).
Mémet, S., Saurin, W. & Sentenac, A. J. biol. Chem. 263, 10048–10051 (1988).
Goodall, G. J. & Filipowicz, W. Cell 58, 473–483 (1989).
Goodall, G. J., Wiebauer, K. & Filipowicz, W. Meth. Enzym. 181 (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Waibel, F., Filipowicz, W. RNA-polymerase specificity of transcription of Arabidopsis U snRNA genes determined by promoter element spacing. Nature 346, 199–202 (1990). https://doi.org/10.1038/346199a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/346199a0
This article is cited by
-
CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil
Scientific Reports (2017)
-
Transcriptional regulation of snRNAs and its significance for plant development
Journal of Plant Research (2017)
-
Treasure hunt in an amoeba: non-coding RNAs in Dictyostelium discoideum
Current Genetics (2007)
-
Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato
Plant Molecular Biology (1996)
-
Gene expression: surprises from the class III side
Molecular and Cellular Biochemistry (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
