Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs

Abstract

To reduce the risks of immunization with killed or live attenuated virus vaccines, it may be advantageous to use a pure, defined antigen that contains determinants for both humoral and cellular immunity. However, although most non-living intact protein pre-parations induce antibodies and CD4+major histocompatibility complex (MHC) class Il-restricted helper and/or cytotoxic T lymphocytes (CTL), they do not elicit CD8+ MHC class I restricted CTL1,2. Indeed, with a few exceptions3,4 it has not so far been possible to induce CD8+ CTL by immunizing with intact soluble proteins. We show here that a single subcutaneous immunization in mice with immunostimulating complexes containing either purified intact gploO envelope glycoprotein of the human immunodeficiency virus (HIV)-l or influenza haemagglutinin results in reproducible and long-lasting priming of HIV specific or influenza-specific CD8+, MHC class I restricted CTL.

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References

  1. 1

    Morrison, L. A., Lukacher, A. E., Braciale, V. L., Fan, D. P. & Braciale, T. J. J. exp. Med. 163, 903–921 (1986).

    CAS  Article  Google Scholar 

  2. 2

    Germain, R. N. Nature 322, 687–689 (1986).

    CAS  Article  ADS  Google Scholar 

  3. 3

    Staerz, U. D., Karasuyama, H. & Garner, A. M. Nature 329, 449–451 (1987).

    CAS  Article  ADS  Google Scholar 

  4. 4

    Wraith, D. C. & Askonas, B. A. J. gen. Virol. 66, 1327–1331 (1985).

    CAS  Article  Google Scholar 

  5. 5

    Morein, B., Sundquist, B., Höglund, S., Dalsgaard, K. & Osterhaus, A. Nature 308, 457–460 (1984).

    CAS  Article  ADS  Google Scholar 

  6. 6

    Takahashi, H. et al. Proc. natn. Acad. Sci. U.S.A. 85, 3105–3109 (1988).

    CAS  Article  ADS  Google Scholar 

  7. 7

    Cease, K. B. et al. Proc. natn. Acad. Sci. U.S.A. 84, 4249–4253 (1987).

    CAS  Article  ADS  Google Scholar 

  8. 8

    Jones, P. D., Tha Hla, R., Morein, B., Lövgren, K. & Ada, G. L. Scand. J. Immun. 27, 645–652 (1988).

    CAS  Article  Google Scholar 

  9. 9

    Townsend, A. R. M. et al. Cell 44, 959–968 (1986).

    CAS  Article  Google Scholar 

  10. 10

    Maryanski, J. L., Pala, P., Corradin, G., Jordan, B. R. & Cerottini, J.-C. Nature 324, 578–579 (1986).

    CAS  Article  ADS  Google Scholar 

  11. 11

    Moore, M. W., Carbone, F. R. & Bevan, M. J. Cell 54, 777–785 (1988).

    CAS  Article  Google Scholar 

  12. 12

    Ishioka, G. Y., Colon, S., Miles, C., Grey, H. M. & Chesnut, R. W. J. Immun. 143, 1094–1100 (1989).

    CAS  PubMed  Google Scholar 

  13. 13

    Takahashi, H. et al. Science 246, 118–121 (1989).

    CAS  Article  ADS  Google Scholar 

  14. 14

    Takahashi, H. et al. J. exp. Med. 170, 2023–2035 (1989).

    CAS  Article  Google Scholar 

  15. 15

    Achour, A. et al. Vth Int. Conf. on AIDS, Montreal 546 abstract, (1989).

  16. 16

    Hildreth, J. E. K. Biochem. J. 207, 363–366 (1982).

    CAS  Article  Google Scholar 

  17. 17

    Javaherian, K. et al. Proc. natn. Acad. Sci. U.S.A. 86, 6768–6772 (1989).

    CAS  Article  ADS  Google Scholar 

  18. 18

    Sarmiento, M., Glasebrook, A. L. & Fitch, F. W. J. Immun. 125, 2665–2672 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. 19

    Ceredig, R., Lowenthal, J. W., Nabholz, M. & MacDonald, H. R. Nature 314, 98–100 (1985).

    CAS  Article  ADS  Google Scholar 

  20. 20

    Margulies, D. H. et al. J. Immun. 130, 463 (1983).

    CAS  Google Scholar 

  21. 21

    Abastado, J.-P. et al. J. exp. Med. 166, 327–340 (1987).

    CAS  Article  Google Scholar 

  22. 22

    Bennink, J. R. & Yewdell, J. W. J. exp. Med. 168, 1935–1939 (1988).

    CAS  Article  Google Scholar 

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Takahashi, H., Takeshfta, T., Morein, B. et al. Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs. Nature 344, 873–875 (1990). https://doi.org/10.1038/344873a0

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