Abstract
To reduce the risks of immunization with killed or live attenuated virus vaccines, it may be advantageous to use a pure, defined antigen that contains determinants for both humoral and cellular immunity. However, although most non-living intact protein pre-parations induce antibodies and CD4+major histocompatibility complex (MHC) class Il-restricted helper and/or cytotoxic T lymphocytes (CTL), they do not elicit CD8+ MHC class I restricted CTL1,2. Indeed, with a few exceptions3,4 it has not so far been possible to induce CD8+ CTL by immunizing with intact soluble proteins. We show here that a single subcutaneous immunization in mice with immunostimulating complexes containing either purified intact gploO envelope glycoprotein of the human immunodeficiency virus (HIV)-l or influenza haemagglutinin results in reproducible and long-lasting priming of HIV specific or influenza-specific CD8+, MHC class I restricted CTL.
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Takahashi, H., Takeshfta, T., Morein, B. et al. Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs. Nature 344, 873–875 (1990). https://doi.org/10.1038/344873a0
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