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A serine protease triad forms the catalytic centre of a triacylglycerol lipase

Nature volume 343, pages 767–770 (1990)Cite this article

Abstract

TRUE Upases attack triacylglycerols and act at an oil–water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.

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Author notes

  1. Lars Christiansen, Birgitte Huge-Jensen, Leif Norskov and Lars Thim: Novo-Nordisk A/S, Novo Alle, DK-2880 Bagsvaerd, Copenhagen, Denmark

  2. Ulrich Menge: Department of Enzyme Technology, GBF, D-3300 Braunschweig, FRG

  3. Zygmunt S. Derewenda: To whom correspondence should be addressed.

Affiliations

  1. Department of Chemistry, University of York, Heslington, York, Y01 5DD, UK

    Leo Brady, Andrzej M. Brzozowski, Zygmunt S. Derewenda, Eleanor Dodson, Guy Dodson, Shirley Tolley, Johan P. Turkenburg, Lars Christiansen, Birgitte Huge-Jensen, Leif Norskov, Lars Thim & Ulrich Menge

  2. Department of Crystallography, Institute of Chemistry, University of Lodz, 91416, Lodz, Poland

    Andrzej M. Brzozowski

Authors
  1. Leo Brady
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  2. Andrzej M. Brzozowski
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  3. Zygmunt S. Derewenda
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  4. Eleanor Dodson
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  5. Guy Dodson
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  6. Shirley Tolley
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  7. Johan P. Turkenburg
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  8. Lars Christiansen
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  9. Birgitte Huge-Jensen
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  10. Leif Norskov
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  11. Lars Thim
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  12. Ulrich Menge
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Brady, L., Brzozowski, A., Derewenda, Z. et al. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343, 767–770 (1990). https://doi.org/10.1038/343767a0

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  • DOI: https://doi.org/10.1038/343767a0

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