Abstract
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.
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References
Krainer, A. R. & Maniatis, T. in Frontiers in Transcription and Splicing (ed. Hames, B. D. & Glover, D. M.) 131–206 (IRL, Oxford and Washington, 1988).
Steitz, J. A. et al. in Structure and Function of Major and Minor Snurps (ed. Birnsteil, M. L.) 115–154 (Springer, Heidelberg, 1988).
Krainer, A. R. & Maniatis, T. Cell 42, 725–736 (1985).
Kramer, A. & Keller, W. EMBO J. 4, 3571–3581 (1985).
Furneaux, H. M., Perkins, K. K., Freyer, G. A., Arenas, J. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 82, 4351–4355 (1985).
Perkins, K. K., Furneaux, H. M. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 83, 887–891 (1986).
Kramer, A., Frick, M. & Keller, W. J. biol. Chem. 262, 17630–17640 (1987).
Ruskin, B., Zamore, P. D. & Green, M. R. Cell 52, 207–219 (1988).
Gerke, V. & Steitz, J. A. Cell 47, 973–984 (1986).
Tazi, J. et al. Cell 47, 755–766 (1986).
Swanson, M. S. & Dreyfuss, G. EMBO J. 7, 3519–3529 (1988).
Garcia-Blanco, M. A., Jamison, S. R. & Sharp, P. A. Genes Dev. 3, 1874–1886 (1989).
Reed, R., Griffith, J. & Maniatis, T. Cell 53, 949–961 (1988).
Abmayr, S. M., Reed, R. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 85, 7216–7220 (1988).
Lerner, E. A., Lerner, M. R., Janeway, C. A. & Steitz, J. A. Proc. natn. Acad. Sci. U.S.A. 78, 2737–2741 (1981).
Deng, J. S., Takasaki, Y. & Tan, E. M. J. Cell Biol. 91, 572–577 (1981).
Spector, D. L., Schrier, W. H. & Busch, H. Biol. Cell 49, 1–10 (1983).
Reuter, R., Appel, B., Bringmann, P., Rinke, J. & Luhrmann, R. Expl Cell Res. 154, 548–560 (1984).
Nyman, U., Hallman, H., Hadlaczky, I., Sharp, G. & Ringertz, N. R. J. Cell Biol. 102, 137–144 (1986).
Chabot, B., Black, D. L., LeMaster, D. M. & Steitz, J. A. Science 230, 1344–1349 (1985).
Krainer, A. R. Nucleic Acids Res. 16, 9415–9429 (1988).
Hinterberger, M., Pettersson, I. & Steitz, J. A. J. biol. Chem. 258, 2604–2613 (1983).
Kinlaw, C. S., Robberson, B. L. & Berget, S. M. J. biol. Chem. 258, 7181–7189 (1983).
Grabowski, P. J., Seiler, S. R. & Sharp, P. A. Cell 42, 345–353 (1985).
Frendewey, D. & Keller, W. Cell 42, 355–367 (1985).
Konarska, M. M. & Sharp, P. A. Cell 46, 845–855 (1986).
Konarska, M. M. & Sharp, P. A. Cell 49, 763–774 (1987).
Bindereif, A. & Green, M. R. EMBO J. 6, 2415–2424 (1987).
Kramer, A. Genes Dev. 2, 1155–1167 (1988).
Mariman, E. C. M., van Eekelen, C. A. G., Reinders, R. J., Berns, A. J. M. & van Venrooji, W. J. J. molec. Biol. 154, 103–119 (1982).
Ciejek, E. M., Nordstrom, J. L., Tsal, M-J. & O'Malley, B. W. Biochemistry 21, 4945–4953 (1982).
Spector, D. L. Biol. Cell 51, 109–112 (1984).
Spector, D. L., Watt, R. A. & Sullivan, N. F. Oncogene 1, 5–12 (1987).
Osheim, Y. N., Miller, O. L. & Beyer, A. L. Cell 43, 143–151 (1985).
Beyer, A. L. & Osheim, Y. N. Genes Dev. 2, 754–765 (1988).
Gall, J. G. & Callan, H. G. Proc. natn. Acad. Sci. U.S.A. 86, 6635–6639 (1989).
Spector, D. L. Proc. natn. Acad. Sci. U.S.A. (in the press).
Fakan, S., Leser, G. & Martin, T. E. J. Cell Biol. 98, 358–363 (1984).
Chang, T-H., Clark, M. W., Lustig, A. J., Cusick, M. E. & Abelson, J. Molec cell. Biol. 8, 2379–2393 (1988).
Bradford, M. M. Analyt. Biochem. 2, 248–254 (1976).
Taggart, R. T. & Samloff, I. M. Science 219, 1228–1230 (1983).
Kiehart, D., Kaiser, D. A. & Pollard, T. D. J. Cell Biol. 99, 1002–1014 (1984).
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Fu, XD., Maniatis, T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437–441 (1990). https://doi.org/10.1038/343437a0
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DOI: https://doi.org/10.1038/343437a0
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