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Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

Nature volume 343, pages 437–441 (1990)Cite this article

Abstract

A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.

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References

  1. Krainer, A. R. & Maniatis, T. in Frontiers in Transcription and Splicing (ed. Hames, B. D. & Glover, D. M.) 131–206 (IRL, Oxford and Washington, 1988).

    Google Scholar 

  2. Steitz, J. A. et al. in Structure and Function of Major and Minor Snurps (ed. Birnsteil, M. L.) 115–154 (Springer, Heidelberg, 1988).

    Google Scholar 

  3. Krainer, A. R. & Maniatis, T. Cell 42, 725–736 (1985).

    Article  CAS  Google Scholar 

  4. Kramer, A. & Keller, W. EMBO J. 4, 3571–3581 (1985).

    Article  CAS  Google Scholar 

  5. Furneaux, H. M., Perkins, K. K., Freyer, G. A., Arenas, J. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 82, 4351–4355 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Perkins, K. K., Furneaux, H. M. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 83, 887–891 (1986).

    Article  ADS  CAS  Google Scholar 

  7. Kramer, A., Frick, M. & Keller, W. J. biol. Chem. 262, 17630–17640 (1987).

    CAS  PubMed  Google Scholar 

  8. Ruskin, B., Zamore, P. D. & Green, M. R. Cell 52, 207–219 (1988).

    Article  CAS  Google Scholar 

  9. Gerke, V. & Steitz, J. A. Cell 47, 973–984 (1986).

    Article  CAS  Google Scholar 

  10. Tazi, J. et al. Cell 47, 755–766 (1986).

    Article  CAS  Google Scholar 

  11. Swanson, M. S. & Dreyfuss, G. EMBO J. 7, 3519–3529 (1988).

    Article  CAS  Google Scholar 

  12. Garcia-Blanco, M. A., Jamison, S. R. & Sharp, P. A. Genes Dev. 3, 1874–1886 (1989).

    Article  CAS  Google Scholar 

  13. Reed, R., Griffith, J. & Maniatis, T. Cell 53, 949–961 (1988).

    Article  CAS  Google Scholar 

  14. Abmayr, S. M., Reed, R. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 85, 7216–7220 (1988).

    Article  ADS  CAS  Google Scholar 

  15. Lerner, E. A., Lerner, M. R., Janeway, C. A. & Steitz, J. A. Proc. natn. Acad. Sci. U.S.A. 78, 2737–2741 (1981).

    Article  ADS  CAS  Google Scholar 

  16. Deng, J. S., Takasaki, Y. & Tan, E. M. J. Cell Biol. 91, 572–577 (1981).

    Article  Google Scholar 

  17. Spector, D. L., Schrier, W. H. & Busch, H. Biol. Cell 49, 1–10 (1983).

    Article  CAS  Google Scholar 

  18. Reuter, R., Appel, B., Bringmann, P., Rinke, J. & Luhrmann, R. Expl Cell Res. 154, 548–560 (1984).

    Article  CAS  Google Scholar 

  19. Nyman, U., Hallman, H., Hadlaczky, I., Sharp, G. & Ringertz, N. R. J. Cell Biol. 102, 137–144 (1986).

    Article  CAS  Google Scholar 

  20. Chabot, B., Black, D. L., LeMaster, D. M. & Steitz, J. A. Science 230, 1344–1349 (1985).

    Article  ADS  CAS  Google Scholar 

  21. Krainer, A. R. Nucleic Acids Res. 16, 9415–9429 (1988).

    Article  CAS  Google Scholar 

  22. Hinterberger, M., Pettersson, I. & Steitz, J. A. J. biol. Chem. 258, 2604–2613 (1983).

    CAS  PubMed  Google Scholar 

  23. Kinlaw, C. S., Robberson, B. L. & Berget, S. M. J. biol. Chem. 258, 7181–7189 (1983).

    CAS  PubMed  Google Scholar 

  24. Grabowski, P. J., Seiler, S. R. & Sharp, P. A. Cell 42, 345–353 (1985).

    Article  CAS  Google Scholar 

  25. Frendewey, D. & Keller, W. Cell 42, 355–367 (1985).

    Article  CAS  Google Scholar 

  26. Konarska, M. M. & Sharp, P. A. Cell 46, 845–855 (1986).

    Article  CAS  Google Scholar 

  27. Konarska, M. M. & Sharp, P. A. Cell 49, 763–774 (1987).

    Article  CAS  Google Scholar 

  28. Bindereif, A. & Green, M. R. EMBO J. 6, 2415–2424 (1987).

    Article  CAS  Google Scholar 

  29. Kramer, A. Genes Dev. 2, 1155–1167 (1988).

    Article  CAS  Google Scholar 

  30. Mariman, E. C. M., van Eekelen, C. A. G., Reinders, R. J., Berns, A. J. M. & van Venrooji, W. J. J. molec. Biol. 154, 103–119 (1982).

    Article  CAS  Google Scholar 

  31. Ciejek, E. M., Nordstrom, J. L., Tsal, M-J. & O'Malley, B. W. Biochemistry 21, 4945–4953 (1982).

    Article  CAS  Google Scholar 

  32. Spector, D. L. Biol. Cell 51, 109–112 (1984).

    Article  CAS  Google Scholar 

  33. Spector, D. L., Watt, R. A. & Sullivan, N. F. Oncogene 1, 5–12 (1987).

    CAS  PubMed  Google Scholar 

  34. Osheim, Y. N., Miller, O. L. & Beyer, A. L. Cell 43, 143–151 (1985).

    Article  CAS  Google Scholar 

  35. Beyer, A. L. & Osheim, Y. N. Genes Dev. 2, 754–765 (1988).

    Article  CAS  Google Scholar 

  36. Gall, J. G. & Callan, H. G. Proc. natn. Acad. Sci. U.S.A. 86, 6635–6639 (1989).

    Article  ADS  CAS  Google Scholar 

  37. Spector, D. L. Proc. natn. Acad. Sci. U.S.A. (in the press).

  38. Fakan, S., Leser, G. & Martin, T. E. J. Cell Biol. 98, 358–363 (1984).

    Article  CAS  Google Scholar 

  39. Chang, T-H., Clark, M. W., Lustig, A. J., Cusick, M. E. & Abelson, J. Molec cell. Biol. 8, 2379–2393 (1988).

    Article  CAS  Google Scholar 

  40. Bradford, M. M. Analyt. Biochem. 2, 248–254 (1976).

    Article  Google Scholar 

  41. Taggart, R. T. & Samloff, I. M. Science 219, 1228–1230 (1983).

    Article  ADS  CAS  Google Scholar 

  42. Kiehart, D., Kaiser, D. A. & Pollard, T. D. J. Cell Biol. 99, 1002–1014 (1984).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    Xiang-Dong Fu & Tom Maniatis

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  1. Xiang-Dong Fu
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  2. Tom Maniatis
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Fu, XD., Maniatis, T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437–441 (1990). https://doi.org/10.1038/343437a0

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  • DOI: https://doi.org/10.1038/343437a0

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