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Goprotein as signal transducer in the pertussis toxin-sensitive phosphatidylinositol pathway

Nature volume 343, pages 79–82 (1990)Cite this article

Abstract

RECEPTORS stimulating phospholipase C do so through heterotrimeric GTP-binding proteins1,2 to produce two second messengers, inositol 1,4,5-trisphosphate (InsP3) and diacylgly-cerol1. In spite of the detailed understanding of phospholipase C structure3 and phosphatidyl inositol signalling1, the identity of the GTP-binding protein involved is so far unknown. To address this issue, we have used the Xenopus oocyte in which muscarinic receptors couple to phospholipase C through a pertussis toxin-sensitive GTP-binding protein2. In this cell, InsP3 mobilizes intracellular Ca21 to evoke a Cl-current. The magnitude of this Cl- current is proportional to the amount of InsP3 in the cell4, and therefore can be used as an assay for InsP3 production. We report here that the activated a-subunit of the GTP-binding protein G0, when directly injected into oocytes, evokes a Cl- current by mobilizing Ca2+ from intracellular InsP3-sensitive stores. We also show that holo-GO, when injected into oocytes, can specifically enhance the muscarinic receptor-stimulated Cl current. These data indicate that GO can serve as the signal transducer of the receptor-regulated phospholipase C inXenopus oocytes.

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References

  1. Berridge, M. J. & Irvine, R. F. Nature 341, 197–204 (1989).

    Article  ADS  CAS  Google Scholar 

  2. Moriarty, T. M. et al. Proc. natn. Acad. Sci. U.S.A. 85, 8865–8869 (1988).

    Article  ADS  CAS  Google Scholar 

  3. Rhee, S. G., Suh, P-G., Ryu, S-H. & Lee, S. Y. Science 244, 546–550 (1989).

    Article  ADS  CAS  Google Scholar 

  4. Gillo, B., Lass, Y., Nadler, E. & Oron, Y. J. Physiol. 392, 349–361 (1987).

    Article  CAS  Google Scholar 

  5. Oron, Y., Dascal, N., Nadler, E. & Lupu, M. Nature 313, 141–143 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Dascal, N. CRC Cril Rev. Biochem. 22, 317–387 (1987).

    Article  CAS  Google Scholar 

  7. Dascal, N., Landau, E. M. & Lass, Y. J. Physiol 352, 551–574 (1984).

    Article  CAS  Google Scholar 

  8. Sugiyama, H., Ito, I. & Hirono, C. Nature 325, 531–533 (1987).

    Article  ADS  CAS  Google Scholar 

  9. Sternwies, P. C. & Gilman, A. G. J. biol. Chem. 254, 3333–3340 (1979).

    Google Scholar 

  10. Gilman, A. G. A. Rev. Biochem. 56, 615–649 (1987).

    Article  CAS  Google Scholar 

  11. Sternweis, P. S. & Robishaw, J. D. J. biol. Chem. 259, 13806–13813 (1984).

    CAS  Google Scholar 

  12. Olate, J. et al. FEBS Lett. 244, 188–192 (1989).

    Article  ADS  CAS  Google Scholar 

  13. Terashima, T., Katada, T., Oinuma, M., Inoue, Y. & Ui, M. Brain Res. 417, 190–194 (1987).

    Article  CAS  Google Scholar 

  14. Mumby, S., Pang, I., Gilman, A. G. & Sternweis, P. C. J. biol. Chem. 263, 2020–2026 (1988).

    CAS  PubMed  Google Scholar 

  15. Rouot, B. et al. Biochem. J. 260, 307–310 (1989).

    Article  CAS  Google Scholar 

  16. Moriarty, T. M. et al. J. biol. Chem. 264, 13524–13530 (1989).

    CAS  PubMed  Google Scholar 

  17. Ashkenai, A., Peralta, E. G., Winslow, J. W., Ramachandran, J. & Capon, D. J. Cell 56, 487–493 (1989).

    Article  Google Scholar 

  18. Masters, S. B. et al. Science 241, 448–451 (1988).

    Article  ADS  CAS  Google Scholar 

  19. Codina, J. et al. J. biol. Chem. 259, 5874–5886 (1984).

    Google Scholar 

  20. Premont, R. T., Buku, A. & Iyengar, R. J. biol. Chem. 264, 14960–14964 (1989).

    CAS  PubMed  Google Scholar 

  21. Codina, J., Yatani, A., Grenet, D., Brown, A. M. & Birnbaumer, L. Science 236, 442–445 (1987).

    Article  ADS  CAS  Google Scholar 

  22. Yatani, A. et al. Nature 336, 680–682 (1988).

    Article  ADS  CAS  Google Scholar 

  23. Kusano, K., Miledi, R. & Stinnakre, J. J. Physiol. 328, 143–170 (1982).

    Article  CAS  Google Scholar 

  24. Barish, M. E. J. Physiol. 342, 309–325 (1983).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Psychiatry and Pharmacology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York, 10029, USA

    Thomas M. Moriarty, Elena Padrell, Donna J. Carty, Gila Omri, Emmanuel M. Landau & Ravi Iyengar

  2. Department of Psychiatry, Bronx Veterans Administration Medical Center, 130 West Kingsbridge Road, Bronx, New York, 10468, USA

    Thomas M. Moriarty, Elena Padrell, Donna J. Carty, Gila Omri, Emmanuel M. Landau & Ravi Iyengar

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  1. Thomas M. Moriarty
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  2. Elena Padrell
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  4. Gila Omri
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  5. Emmanuel M. Landau
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  6. Ravi Iyengar
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Moriarty, T., Padrell, E., Carty, D. et al. Goprotein as signal transducer in the pertussis toxin-sensitive phosphatidylinositol pathway. Nature 343, 79–82 (1990). https://doi.org/10.1038/343079a0

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