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Molecular cloning and expression of human hepatocyte growth factor


HEPATOCYTE growth factor (HGF) is the most potent mitogen for mature parenchyma! hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification and characterization of HGF have been reported1–4. We have demonstrated that pure HGF from rat platelets is a new growth factor5, 6 effective at concentrations as low as 1 ng mP−1.The effects of HGF and epidermal growth factor (EGF) are additive. The activity of HGF is not species-specific, although it does not stimulate growth in Swiss 3T3 fibroblasts5. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large a-subunit of Mr 69,000 and a small β -subunit of Mr34,000 (ref. 6). Here we report the amino-acid sequence of human HGF determined by complementary DNA cloning and the expression of biologically active human HGF from COS-1 cells transfected with cloned cDNA. The nucleotide sequence of the human HGF cDNA reveals that both α- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids.

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  1. Nakamura, T., Nawa, K. & Ichihara, A. Biochem. biophys. Res. Commun. 122, 1450–1459 (1984).

    CAS  Article  Google Scholar 

  2. Thaler, F. J. & Michalopoulos, G. K. Cancer Res. 45, 2545–2549 (1985).

    CAS  PubMed  Google Scholar 

  3. Ghoda, E. et al. J. clin. Invest 81, 414–419 (1988).

    Article  Google Scholar 

  4. Rusell, W. E., McGowan, J. A. & Bucher, N. R. L. J. Cell Physiol. 119, 193–197 (1984).

    Article  Google Scholar 

  5. Nakamura, T., Teramoto, H. & Ichihara, A. Proc. natn. Acad. Sci. U.S.A. 83, 6489–6493 (1986).

    ADS  CAS  Article  Google Scholar 

  6. Nakamura, T., Nawa, K., Ichihara, A., Kaise, N. & Nishino, T. FEBS Lett. 224, 311–316 (1987).

    CAS  Article  Google Scholar 

  7. Von Heijine, G. Eur. J. Biochem. 133, 17–21 (1983).

    Article  Google Scholar 

  8. Kawabata, S. et al. Eur. J. Biochem. 172, 17–25 (1988).

    CAS  Article  Google Scholar 

  9. Sottrup-Jensen, L., Claeys, H., Zajdel, M., Petersen, T. E. & Magnusson, S. Progress in Chemical Fibrinolysis and Thrombolysis Vol. 3, 191–209 (Raven, New York, 1978).

    Google Scholar 

  10. Furie, B. & Furie, B. C. Cell 53, 505–518 (1988).

    CAS  Article  Google Scholar 

  11. Kurosky, A. et al. Proc. natn Acad. Sci. U.S.A. 77, 3388–3392 (1980).

    ADS  CAS  Article  Google Scholar 

  12. Hørjup, P., Jensen, M. S. & Petersen, T. E. FEBS Lett. 184, 333–338 (1985).

    Article  Google Scholar 

  13. Morley, C. G. D. & Kingdom, H. S. Biochim. biophys. Acta 308, 260–275 (1973).

    CAS  Article  Google Scholar 

  14. Morley, C. G. D. & Boyer, J. L. Biochim. biophys. Acta 477, 165–176 (1977).

    CAS  Article  Google Scholar 

  15. Ando T. et al. Hepatology (in the press).

  16. Nakayama, H. et al. Biomed. Res. 6, 231–237 (1985).

    MathSciNet  CAS  Article  Google Scholar 

  17. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual 202–203 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  18. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual 125–126 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  19. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    CAS  Article  Google Scholar 

  20. Huynh, T. V., Young, R. A. & Davis, R. W. DNA Cloning Vol. 1, 49–78 (IRL, Oxford, 1985).

    Google Scholar 

  21. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    ADS  CAS  Article  Google Scholar 

  22. Sanger, F., Miklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    ADS  CAS  Article  Google Scholar 

  23. Berger, S. L. & Kimmel, A. R. Meth. Enzym. 102, 684–704 (Academic, London, 1987).

    Google Scholar 

  24. Nakamura, T., Tomita, Y. & Ichihara, A. J. Biochem. 94, 1029–1035 (1983).

    CAS  Article  Google Scholar 

  25. Nakamura, T., Arakaki, R. & Ichihara, A. Expl. Cell Res. 179, 488–497 (1988).

    CAS  Article  Google Scholar 

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Nakamura, T., Nishizawa, T., Hagiya, M. et al. Molecular cloning and expression of human hepatocyte growth factor. Nature 342, 440–443 (1989).

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