Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans

Nature volume 342, pages 45–50 (1989)Cite this article

Abstract

The Caenorhabditis elegans gene unc-22 encodes a very large muscle protein, called twitchin, which consists of a protein kinase domain and several copies of two short motifs. The sequence of twit-chin has unexpected similarities to the sequences of proteins of the immunoglobulin superfamily, cell adhesion molecules and vertebrate muscle proteins, including myosin light-chain kinase. These homologies, together with results from earlier genetic and molecular analyses, indicate that twit-chin is involved in a novel mechanism of myosin regulation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Waterston, R. H. in The Nematode Caenorhabditis elegans (ed. Wood, W.B.) 281–335 (Cold Spring Harbor Laboratory, New York, 1988).

    Google Scholar 

  2. Waterston, R. H., Thomson, J. N. & Brenner, S. Devl Biol. 77, 271–302 (1980).

    Article  CAS  Google Scholar 

  3. Moerman, D. G. thesis, Simon Fraser Univ. Burnaby, British Columbia (1980).

  4. Moerman, D. G., Plurad, S., Waterston, R. H. & Baillie, D. L. Cell 29, 773–781 (1982).

    Article  CAS  Google Scholar 

  5. Dibb, N. J. et al. J. molec Biol. 183, 543–551 (1985).

    Article  CAS  Google Scholar 

  6. Moerman, D. G., Benian, G. M. & Waterston, R. H. Proc. natn. Acad. Sci. U.S.A. 83, 2579–2583 (1986).

    Article  ADS  CAS  Google Scholar 

  7. Moerman, D. G., Benian, G. M., Barstead, R. J., Schriefer, L. A. & Waterston, R. H. Genes Dev. 2, 93–105 (1988).

    Article  CAS  Google Scholar 

  8. Maruyama, K., Kimura, S., Yoshidomi, H., Sawada, H. & Kikuchi, M. J. Biochem. 95, 1423–1433 (1984).

    Article  CAS  Google Scholar 

  9. Kurzban, G. P. & Wang, K. Biochem. biophys. Res. Commun. 150, 1155–1161 (1988).

    Article  CAS  Google Scholar 

  10. Wang, K., McClure, J. & Tu, A. Proc. natn. Acad. Sci. U.S.A. 76, 3698–3702 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Whiting, A., Wardale, J. & Trinick, J. J. molec. Biol. 205, 263–268 (1989).

    Article  CAS  Google Scholar 

  12. Wang, K., Ramirez-Mitchell, R. & Palter, D. Proc. natn. Acad. Sci. U.S.A. 81, 3685–3689 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Trinick, J., Knight, P. & Whiting, A. J. molec. Biol. 180, 331–356 (1984).

    Article  CAS  Google Scholar 

  14. Wang, K. & Williamson, C. L. Proc. natn. Acad. Sci. U.S.A. 77, 3254–3258 (1980).

    Article  ADS  CAS  Google Scholar 

  15. Koenig, M., Monaco, A. P., & Kunkel, L. M. Cell 53, 219–228 (1988).

    Article  CAS  Google Scholar 

  16. Coulson, A., Sulston, J., Brenner, S. & Karn, J. Proc. natn. Acad. Sci. U.S.A. 83, 7821–7825 (1986).

    Article  ADS  CAS  Google Scholar 

  17. Mori, I., Benian, G. M., Moerman, D. G. & Waterston, R. H. Proc. natn. Acad. Sci. U.S.A. 85, 861–864 (1988).

    Article  ADS  CAS  Google Scholar 

  18. Emmons, S. W. in The Nematode Caenorhabditis elegans (ed. Wood, W. B.) 57–59 (Cold Spring Harbor Laboratory, New York, 1988).

    Google Scholar 

  19. Blumenthal, T. & Thomas, J. Trends Genet. 4, 305–308 (1988).

    Article  CAS  Google Scholar 

  20. Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–52 (1988).

    Article  ADS  CAS  Google Scholar 

  21. Guerriero, V., Russo, M. A., Olson, N. J., Putkey, J. A. & Means, A. R. Biochemistry 25, 8372–8381 (1986).

    Article  CAS  Google Scholar 

  22. Takio, K., Blumenthal, D. K., Walsh, K. A., Titani, K. & Krebs, E. G. Biochemistry 25, 8049–8057 (1986).

    Article  CAS  Google Scholar 

  23. Williams, A. F. & Barclay, A. N. A. Rev. Immun. 6, 381–405 (1988).

    Article  CAS  Google Scholar 

  24. Cunningham, B. A. et al. Science 236, 799–806 (1987).

    Article  ADS  CAS  Google Scholar 

  25. Harrelson, A. L. & Goodman, C. S. Science 242, 700–708 (1988).

    Article  ADS  CAS  Google Scholar 

  26. Moos, M. et al. Nature 334, 701–703 (1988).

    Article  ADS  CAS  Google Scholar 

  27. Kornblihtt, A. R., Umezawa, K., Vibe-Pedersen, K. & Baralle, F. E. EMBO J. 4, 1755–1759 (1985).

    Article  CAS  Google Scholar 

  28. Schiffer, M., Girling, R. L., Ely, K. R. & Edmundson, A. B. Biochemistry 12, 4620–4631 (1973).

    Article  CAS  Google Scholar 

  29. Edelman, A. M., Blumenthal, D. K. & Krebs, E. G. A. Rev. Biochem. 56, 567–613 (1987).

    Article  CAS  Google Scholar 

  30. Kamm, K. E. & Stull, J. T. A. Rev. Pharmacol. Tox. 25, 593–620 (1985).

    Article  CAS  Google Scholar 

  31. Harris, H. E., Tso, M.-Y. W. & Epstein, H. F. Biochemistry 16, 859–865 (1977).

    Article  CAS  Google Scholar 

  32. Lehman, W. & Szent-Gyorgyi, A. G. J. gen. Physiol. 66, 1–30 (1975).

    Article  CAS  Google Scholar 

  33. McLachlan, A. D. & Karn, J. Nature 299, 226–231 (1982).

    Article  ADS  CAS  Google Scholar 

  34. Kagawa, H., Gengyo, K., McLachlan, A. D., Brenner, S. & Karn, J. J. molec. Biol. 207, 311–333 (1989).

    Article  CAS  Google Scholar 

  35. Kiff, J. E., Moerman, D. G., Schriefer, L. A. & Waterston, R. H. Nature 331, 631–633 (1988).

    Article  ADS  CAS  Google Scholar 

  36. Moerman, D. G. & Baillie, D. L. Genetics 91, 95–103 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  37. Moerman, D. G. & Waterston, R. H. Genetics 108, 859–877 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  38. Rogalski, T. M. & Baillie, D. L. Molec. Gen. Genet. 201, 409–414 (1985).

    Article  CAS  Google Scholar 

  39. Bankier, A. T. & Barren, B. G. in Techniques in the Life Sciences Vol. B508 (ed. Flavell, R. A.) 1–34 (Elsevier, Ireland, 1983).

    Google Scholar 

  40. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  41. Tabor, S. & Richardson, C. C. Proc. natn. Acad. Sci. U.S.A. 84, 4767–4771 (1987).

    Article  ADS  CAS  Google Scholar 

  42. Ansorge, W. & Labeit, S. J. Biochem. biophys. Meth. 10, 237–243 (1984).

    Article  CAS  Google Scholar 

  43. Staden, R. Nucleic Acids Res. 14, 217–231 (1986).

    Article  CAS  Google Scholar 

  44. Arquint, M. et al. Proc. Acad. Sci. U.S.A. 84, 600–604 (1987).

    Article  ADS  CAS  Google Scholar 

  45. Yarden, Y. et al. Nature 323, 226–232 (1986).

    Article  ADS  CAS  Google Scholar 

  46. Lewis, V. A., Koch, T., Plutner, H. & Mellman, I. Nature 324, 372–375 (1986).

    Article  ADS  CAS  Google Scholar 

  47. Needleman, S. B. & Wunsch, D. C. J. molec. Biol. 48, 545–552 (1970).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Jane E. Kiff

    Present address: Division of Biological Sciences, University of Missouri, Columbia, Missouri, 65211, USA

  2. Donald G. Moerman

    Present address: Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 2A9, Canada

Authors and Affiliations

  1. Department of Pathology, Woodruff Memorial Building, Emory University School of Medicine, Atlanta, Georgia, 30322, USA

    Guy M. Benian & Nicolas Neckelmann

  2. Department of Genetics, Washington University School of Medicine, St Louis, Missouri, 63110, USA

    Jane E. Kiff, Donald G. Moerman & Robert H. Waterston

Authors
  1. Guy M. Benian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jane E. Kiff
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nicolas Neckelmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Donald G. Moerman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert H. Waterston
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Benian, G., Kiff, J., Neckelmann, N. et al. Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans. Nature 342, 45–50 (1989). https://doi.org/10.1038/342045a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/342045a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing