Abstract
ALTHOUGH single heterozygous markers in yeast usually segregate during meiosis in a 2:2 ratio, abberant 3:1 segregations occur quite frequently as a result of gene-conversion events1. A second type of aberrant segregation, post-meiotic segregation, results from the segregation of two genotypes from a single haploid spore; in yeast such events are detected as sectored spore colonies2 and usually occur rarely1. Post-meiotic segregation is thought to result from the replication of heteroduplex DNA formed during meiotic recombination. We report here that if the heteroduplex includes a palindromic insertion sequence, a high frequency of post-meiotic segregation results. This suggests that palindromic insertions are poorly repaired, which may be the result of hairpin-loop formation that affects the efficiency of repair of heteroduplex DNA.
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Nag, D., White, M. & Petes, T. Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast. Nature 340, 318–320 (1989). https://doi.org/10.1038/340318a0
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DOI: https://doi.org/10.1038/340318a0
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