Abstract
ATRIAL natriuretic peptide (ANP) is a polypeptide hormone whose effects include the induction of diuresis, natriuresis and vasorelaxation1. One of the earliest events following binding of ANP to receptors on target cells is an increase in cyclic GMP concentration, indicating that this nucleotide might act as a mediator of the physiological effects of the hormone2,3. Guanylate cyclase exists in at least two different molecular forms: a soluble haem-containing enzyme consisting of two summits4,5 and a non-haem-containing transmembrane protein having a single subunit6. It is the membrane form of guanylate cyclase that is activated following binding of ANP to target cells3,7,8. We report here the isolation, sequence and expression of a complementary DNA clone encoding the membrane form of guanylate cyclase from rat brain. Transfec-tion of this cDNA into cultured mammalian cells results in expression of guanylate cyclase activity and ANP-binding activity. The ANP receptor/guanylate cyclase represents a new class of mammalian cell-surface receptors which contain an extracellular ligand-binding domain and an intracellular guanylate cyclase catalytic domain.
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References
Flynn, T. G & Davies, P. L. Biochem. J. 232, 313–321 (1985).
Hamet, P. et al. Biochem. biophys. Res. Commun. 123, 515–527 (1984).
Waldman, S. A., Rapoport, R. M. & Murad, F. J. biol. Chem. 259, 14332–14334 (1984).
Gerzer, R., Bohme, E., Hoffman, F. & Schultz, G. FEBS Lett. 132, 71–74 (1981).
Kamisaki, Y. et al. J. biol. Chem. 261, 7236–7241 (1986).
Singh, S. et al. Nature 334, 708–712 (1988).
Tremblay, J. et al. FEBS Lett. 181, 17–22 (1985).
Winquist, R. J. et al. Proc. natn. Acad. Sci. U.S.A. 81, 7661–7664 (1984).
Von Heijne, G. Eur. J. Biochem. 133, 17–21 (1983).
Radany, E. W., Gerzer, R. & Garbers, D.L. J. biol. Chem. 258, 8346–8351 (1983).
Ramarao, C. S. & Garbers, D. L. J. biol. Chem. 263, 1524–1529 (1988).
Kuno, T. et al. J. biol. Chem. 261, 5817–5823 (1986).
Paul, A. K., Marala, R. B., Jaiswal, R. K. & Sharma, R. K. Science 235, 1224–1226 (1987).
Maack, T. et al. Science 238, 675–678 (1987).
Leitman, D. C. et al. J. biol. Chem. 263, 3720–3728 (1988).
Fuller, F. et al. J. biol. Chem. 263, 9395–9401 (1988).
Yarden, Y. et al. Nature 323, 226–232 (1986).
Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–53 (1988).
Koesling, D. et al. FEBS Lett 239, 29–34 (1988).
Kataoka, T., Broek, D. & Wigler, M. Cell 43, 493–505 (1985).
Kozak, M. Nucleic Acids Res. 12, 3873–3893 (1984).
Mueller, P. P. & Hinnebusch, A. G. Cell 45, 201–207 (1986).
Yip, C. C., Laing, L. D. & Flynn, T. G. J. biol. Chem. 260, 8229–8232 (1985).
Takayanagi, R. et al. J. biol. Chem. 262, 12104–12113 (1987).
Shimomura, H., Dangott, L. J. & Garbers, D.L. J. biol. Chem. 261, 15778–15782 (1986).
Hansbrough, J. R. & Garbers, D. L. J. biol. Chem. 256, 1447–1452 (1981).
Bentley, J. K., Tubb, D. J. & Garbers, D. L. J. biol. Chem. 261, 14859–14862 (1986).
Cullen, B. R. Meth. Enzym. 152, 684–704 (1987).
Laemmli, U. K. Nature 227, 680–685 (1970).
Ullrich, A. et al. Nature 309, 418–425 (1984).
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Chinkers, M., Garbers, D., Chang, MS. et al. A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature 338, 78–83 (1989). https://doi.org/10.1038/338078a0
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DOI: https://doi.org/10.1038/338078a0
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