Abstract
B lymphocytes can be rendered specifically unresponsive to antigen by experimental manipulation in vivo and in vitro1–6, but it remains unclear whether or not natural tolerance involves B-cell tolerance because B cells are controlled by T lymphocytes, and in their absence respond poorly to antigen (reviewed in ref. 7). In addition, autoantibody-producing cells can be found in normal mice and their formation is enhanced by B-cell mitogens such as lipopolysaccharides8–12. We have studied B-cell tolerance in transgenic mice using genes for IgM anti-H–2k MHC class I antibody. In H–2d transgenic mice about 25–50% of the splenic B cells bear membrane immunoglobulin of this specificity, and abundant serum IgM encoded by the transgenes is produced. In contrast, H–2k x H–2d (H–2-d/k) transgenic mice lack B cells bearing the anti-H–2k idiotype and contain no detectable serum anti-H–2k antibody, suggesting that very large numbers of autospecific B cells can be controlled by clonal deletion.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dresser, D. W. Immunology 5, 378–388 (1962).
Chiller, J. M., Habicht, G. S. & Weigel, W. O. Science 171, 813–815 (1970).
Borel, Y. Nature new Biol. 230, 180 (1971).
Etlinger, H. M. & Chiller, J. M. Cell. Immun. 33, 297–308 (1977).
Teale, J. M. & Klinman, N. R. Nature 288, 385–387 (1980).
Pike, B. L., Kay, T. W. & Nossal, G. J. V. J. exp. Med. 152, 1407–1412 (1980).
Nossal, G. J. V. Proc. R. Soc. 288, 225–240 (1986).
Moller, G. et al. J. exp. Med. 143, 1429–1438 (1982).
Harris, D. E., Cairns, L., Rosen, F. S. & Borel, Y. J. exp. Med. 156, 567–584 (1982).
Jemmerson, R. R. W., Morrow, P. R., Klinman, N. R. & Patterson, Y. Proc. natn. Acad. Sci. U.S.A. 82, 1508–1512 (1985).
Karray, S., Lymberi, P., Avranmeas, S. & Continho, A. Scand. J. Immun. 23, 475–480 (1986).
Rolink, A. G., Radaszkiewicz, T. & Melchers, F. J. exp. Med. 165, 1675–1687 (1987).
Ozato, K., Mayer, N. & Sachs, D. H. J. Immun. 124, 533–540 (1980).
Nemazee, D. A. in Proc. EMBO Workshop on Tolerance (eds Matzinger, P. et al.) Vol. 2, 52–54 (Editiones Roche, Basle, 1987).
Sidman, C. & Unanue, E. Nature 257, 149–151 (1975).
Storb, U. A. Rev. Immun. 5, 151–174 (1987).
Goodnow, C. C. et al. Nature 334, 676–682 (1988).
Kappler, J. W., Roehm, N. & Marrack, P. Cell 49, 273–280 (1987).
Bretscher, P. & Cohn, M. Science 169, 1042–1049 (1970).
Steinmetz, M. et al. Cell 24, 125–134 (1981).
Southern, P. J. & Berg, P. J. molec. appl. Genet. 1, 327–341 (1982).
Marcu, K. B. et al. Cell 22, 187–196 (1980).
Gordon, J. W. & Ruddle, F. W. Meth. Enzym. 101, 411–433 (1983).
Bürki, K. Mon. Devl Biol. 19, 1–73 (1986).
Brinster, R. L., Chen, H. Y., Trumbauer, M. E., Yagle, M. K. & Palmiter, R. D. Proc. natn. Acad. Sci. U.S.A. 82, 4438–4442 (1985).
Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: a Laboratory Manual 196–203 (Cold Spring Harbor Laboratory, New York, 1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nemazee, D., Bürki, K. Clonal deletion of B lymphocytes in a transgenic mouse bearing anti-MHC class I antibody genes. Nature 337, 562–566 (1989). https://doi.org/10.1038/337562a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/337562a0
This article is cited by
-
B cell-reactive triad of B cells, follicular helper and regulatory T cells at homeostasis
Cell Research (2024)
-
Mechanismen immunologischer Toleranz und ihrer Dysregulation bei rheumatischen Erkrankungen
Zeitschrift für Rheumatologie (2023)
-
A novel transgenic mouse strain expressing PKCβII demonstrates expansion of B1 and marginal zone B cell populations
Scientific Reports (2020)
-
Metabolic gatekeepers to safeguard against autoimmunity and oncogenic B cell transformation
Nature Reviews Immunology (2019)
-
Poly- and autoreactivity of HIV-1 bNAbs: implications for vaccine design
Retrovirology (2018)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.