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Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate

Abstract

cdc2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells, cdc2 is shown to be the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell-cycle regulation. One site ofcdc2 tyrosine phosphorylation in vivo W selectively phosphorylated by pp60c–src in vitro.

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