Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides

Abstract

Murine embryonic stem (ES) cells are pluripotent cell lines established directly from the early embryo1,2 which can contribute differentiated progeny to all adult tissues, including the germ-cell lineage3, after re-incorporation into the normal embryo. They provide both a cellular vector for the generation of transgenic animals4 and a useful system for the identification of polypeptide factors controlling differentiation processes in early development5. In particular, medium conditioned by Buffalo rat liver cells contains a polypeptide factor, ES cell differentiation inhibitory activity (DIA), which specifically suppresses the spontaneous differentiation of ES cells in vitro, thereby permitting their growth as homogeneous stem cell populations in the absence of heterologous feeder cells6. ES cell pluripotentiality, including the ability to give rise to functional gametes, is preserved after prolonged culture in Buffalo rat liver media as a source of DIA7. Here, we report that purified DIA is related in structure and function to the recently identified haemopoetic regulatory factors human interleukin for DA cells8,9 and leukaemia inhibitory factor10. DIA and human interleukin DA/leukaemia inhibitory factor have thus been identified as related multifunctional regulatory factors with distinct biological activities in both early embryonic and haemopoetic stem cell systems.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Evans, M. J. & Kaufman, M. Nature 292, 154–156 (1981).

    ADS  CAS  Article  Google Scholar 

  2. Martin, G. R. Proc. natn. Acad. Sci. U.S.A. 78, 7634–7638 (1981).

    ADS  CAS  Article  Google Scholar 

  3. Bradley, A., Evans, M., Kaufman, M. H. & Robertson, E. Nature 309, 255–256 (1984).

    ADS  CAS  Article  Google Scholar 

  4. Robertson, E. J. Trends Genet. 2, 9–13 (1986).

    Article  Google Scholar 

  5. Heath, J. K., Smith, A. G., Wills, A. J. & Edwards J. Cell Sci. Sup (in the press).

  6. Smith, A. G. & Hooper, M. L. Devl Biol. 121, 1–9 (1987).

    CAS  Article  Google Scholar 

  7. Hooper, M., Hardy, K., Handyside, A., Hunter, S. & Monk, M. Nature 326, 292–2857 (1987).

    ADS  CAS  Article  Google Scholar 

  8. Moreau, J. F. et al. Annls Inst. Pasteur, Immunol. 137, 25–37 (1986).

    Article  Google Scholar 

  9. Moreau, J. F. et al. J. Immun. 138, 3844–3849 (1987).

    CAS  PubMed  Google Scholar 

  10. Gearing, D. P. et al. EMBO J. 6, 3995–4002 (1987).

    CAS  Article  Google Scholar 

  11. Solter, D. & Knowles, B. Proc. natn. Acad. Sci. U.S.A. 75, 5565–5569 (1978).

    ADS  CAS  Article  Google Scholar 

  12. Moreau, J-F. et al. Nature 336, 690–692 (1988).

    ADS  CAS  Article  Google Scholar 

  13. Gough, N. M. et al. Proc. natn. Acad. Sci. U.S.A. 85, 2623–2627 (1988).

    ADS  CAS  Article  Google Scholar 

  14. Williams, R. L. et al. Nature 336, 684–687 (1988).

    ADS  CAS  Article  Google Scholar 

  15. Laemmli, U. K. Nature 227, 680–685 (1970).

    ADS  CAS  Article  Google Scholar 

  16. Mendel, C. M. & Mendel, D. B. Biochem. J. 228, 269–272 (1985).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Smith, A., Heath, J., Donaldson, D. et al. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336, 688–690 (1988). https://doi.org/10.1038/336688a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/336688a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing