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Laminin receptor on platelets is the integrin VLA-6

Nature volume 336, pages 487–489 (1988)Cite this article

Abstract

Adhesion of platelets to the subendothelial matrix of an injured vessel wall is an essential step in triggering the formation of a haemostatic plug. Fibronectin, collagen and laminin are three major components of the subendothelial matrix which support platelet adhesion1. Receptors for fibronectin and collagen have been identified on platelets and are included in the integrin family2–11. Here we report that adhesion of platelets to laminin is inhibited by a rat monoclonal antibody against the integrin family member, VLA-6. This antibody does not affect platelet adhesion to fibrinogen, fibronectin or to type I and III collagen. Binding to laminin does not require platelet activation and is not inhibited by fibronectin and laminin cell-attachment peptides. Platelet adhesion to laminin is supported by Mn2+, Co2+and Mg2+, but not by Ca2+, Zn2+ and Cu2+. This cation preference is distinct from that characteristic for other platelet-adhesive glycoproteins8,12,13.

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References

  1. III, C. R., Engvall, E. & Ruoslahti, E. J. Cell Biol. 99, 2138–2145 (1984).

    Google Scholar 

  2. Gardner, J. M. & Hynes, R. O. Cell 42, 439–448 (1985).

    Article  CAS  PubMed  Google Scholar 

  3. Plow, E. F., McEver, R. P., Coller, B. S., Woods, V. L. Jr & Marguerie, G. A. Blood 66, 724–727 (1985).

    CAS  PubMed  Google Scholar 

  4. Pytela, R., Pierschbacher, M. D., Ginsberg, M. H., Plow, E. F. & Ruoslahti, E. Science 231, 1559–1562 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Giancotti, F. P. et al. Blood 69, 1535–1538 (1987).

    CAS  PubMed  Google Scholar 

  6. Piotrowicz, R. S., Orchekowski, R. P., Nugent, D. J., Yamada, K. Y. & Kunicki, T. J. J. Cell Biol 106, 1359–1364 (1988).

    Article  CAS  PubMed  Google Scholar 

  7. Nieuwenhuis, H. K., Akkerman, J. W. N., Houdijk, W. P. M. & Sixma, J. J. Nature 318, 470–472 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Santoro, S. A. Cell 46, 913–920 (1986).

    Article  CAS  PubMed  Google Scholar 

  9. Santoro, S. A., Rajpara, S. M., Staatz, W. D. & Woods, V. J. Jr Biochem. biophys. Res. Commun. 153, 217–223 (1988).

    Article  CAS  PubMed  Google Scholar 

  10. Kunicki, T. J. et al. J. biol. Chem. 263, 4516–4519 (1988).

    CAS  PubMed  Google Scholar 

  11. Hemler, M. E., Crouse, C., Takada, Y. & Sonnenberg, A. J. biol. Chem. 263, 7660–7665 (1988).

    CAS  PubMed  Google Scholar 

  12. Bennett, J. S. & Vilaire, G. J. clin. Invest. 64, 1393–1401 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Marguerie, G. A., Edgington, T. S. & Plow, E. F. J. biol. Chem. 255, 154–161 (1980).

    CAS  PubMed  Google Scholar 

  14. Hynes, R. O. Cell 48, 549–554 (1987).

    Article  CAS  PubMed  Google Scholar 

  15. Ruoslahti, E. & Pierschbacher, M. D. Science 238, 491–497 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Jennings, L. & Phillips, D. R. J. biol. Chem. 257, 10458–10463 (1982).

    CAS  PubMed  Google Scholar 

  17. Pischel, K. D., Bluestein, H. G. & Woods, V. L. J. clin. Invest. 81, 505–513 (1987).

    Article  Google Scholar 

  18. Pytela, R., Pierschbacher, M. D. & Ruoslahti, E. Cell 40, 191–198 (1985).

    Article  CAS  PubMed  Google Scholar 

  19. Takada, Y., Huang, C. & Hemler, M. E. Nature 326, 607–609 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Sonnenberg, A., Janssen, H., Hogervorst, F., Calafat, J. & Hilgers, J. J. biol. Chem. 262, 10376–10383 (1987).

    CAS  PubMed  Google Scholar 

  21. Rao, N. C., Barsky, S. H., Terranova, V. P. & Liotta, L. A. Biochem. biophys. Res. Commun. 111, 804–808 (1983).

    Article  CAS  PubMed  Google Scholar 

  22. Malinoff, H. L. & Wicha, M. S. J. Cell Biol. 96, 1475–1479 (1983).

    Article  CAS  PubMed  Google Scholar 

  23. Lesot, H., Kühl, U. & von der Mark, K. EMBO J. 2, 861–865 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Wayner, E. A. & Carter, W. G. J. Cell. Biol. 105, 1873–1884 (1987).

    Article  CAS  PubMed  Google Scholar 

  25. Graf, J. et al. Cell 48, 989–996 (1987).

    Article  CAS  PubMed  Google Scholar 

  26. Modderman, P. W., Huisman, H. G., van Mourik, J. A. & von dem Borne, A. E. G. Kr. Thromb. Haemostas 60, 68–74 (1988).

    Article  CAS  Google Scholar 

  27. Giltay, J. C., Brinkman, H. J. M., Modderman, P. W., von dem Borne, A. E. G. Kr. & van Mourik, J. A. Blood (submitted).

  28. Cheresh, D. A. et al. J. Cell Biol. 105, 1163–1173 (1987).

    Article  CAS  PubMed  Google Scholar 

  29. Edgar, D., Timpl, R. & Thoenen, H. EMBO J. 3, 1463–1468 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Engvall, E. et al. J. Cell Biol. 103, 2457–2465 (1986).

    Article  CAS  PubMed  Google Scholar 

  31. Legrand, C., Dubernard, V. & Nurden, A. T. Biochim. biophys. Acta 812, 802–810 (1985).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The Netherlands

    Arnoud Sonnenberg & Piet W. Modderman

  2. The Netherlands Cancer Institute, Amsterdam, The Netherlands

    Frans Hogervorst

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  1. Arnoud Sonnenberg
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  2. Piet W. Modderman
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  3. Frans Hogervorst
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Sonnenberg, A., Modderman, P. & Hogervorst, F. Laminin receptor on platelets is the integrin VLA-6. Nature 336, 487–489 (1988). https://doi.org/10.1038/336487a0

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