Abstract
The vasa gene product of Drosophila melanogaster is required only in the female germ line. Progeny of females homozygous for vasa mutations lack posterior structures and pole cells. Isolation and characterization of vasa genomic and complementary DNA clones show that the transcript is abundant in the female germ line and early embryos only. The predicted amino acid sequence is very similar to those of the translation initiation factor eIF-4A and the human nuclear antigen p68.
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References
Schüpbach, T. & Wieschaus, E. Wilhelm Roux Arch dev. Biol. 195, 302–317 (1986).
Frohnhöfer, H.-G. & Nüsslein-Volhard, C. Nature 324, 120–125 (1986).
Frohnhöfer, H.-G. & Nüsslein-Volhard, C. Genes Devl 1, 880–890 (1987).
Frigerio, G., Burri, M., Bopp, D., Baumgartner, S. & Noll, M. Cell 47, 735–746 (1986).
Berleth, T. et al. EMBO J. 7, 1749–1756 (1988).
Stephenson, E. C. & Mahowald, A. P. Devl Biol. 124, 1–8 (1987).
Driever, W. & Nüsslein-Volhard, C. Cell 54, 83–93 95–104 (1988).
Nüsslein-Volhard, C., Frohnhöfer, H.-G. & Lehmann, R. Science 238, 1675–1681 (1987).
Boswell, R. & Mahowald, A. Cell 43, 97–104 (1985).
Lehmann, R. & Nüsslein-Volhard, C. Cell 47, 141–152 (1986).
Lehmann, R. & Nüsslein-Volhard, C. Nature 329, 167–170 (1987).
King, R. C. Ovarian Development in Drosophila melanogaster (Academic, New York, 1970).
Mahowald, A. P. & Kambysellis, M. P. in The Genetics and Biology of Drosophila (eds Ashburner, M. & Wright, T. R. W.) 2d, 141–224 (Academic, London, 1980).
Nielsen, P. J., McMaster, G. K. & Trachsel, H. Nucleic Acids Res. 13, 6867–6880 (1985).
Ford, M. J., Anton, I. A. & Lane, D. P. Nature 332, 736–738 (1988).
Woodruff, R. C., & Ashburner, M. Genetics 92, 117–132 (1979).
Ashburner, M. et al. (manuscript in preparation).
Chia, W., Karp, M., McGill, S. & Ashburner, M. J. molec. Biol. 186, 689–706 (1985).
Frischauf, A.-M., Lehrach, H., Poutska, A. & Murray, N. J. molec. Biol. 170, 827–842 (1983).
Cavener, D. R. Nucleic Acids Res. 15, 1353–1361 (1987).
Keller, E. B. & Noon, W. A. Nucleic Acids Res. 13, 4971–4981 (1985).
Lipman, D. J. & Pearson, W. R. Science 227, 1435–1441 (1985).
Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945–951 (1982).
Higgins, C. F., Gallagher, M. P., Mimmack, M. L. & Pearce, S. R. Bioessays 8, 111–116 (1988).
Hodgman, T. C. Nature 333, 22–23 (1988).
Lane, D. Nature 334, 478 (1988).
Hodgman, T. C. Nature 333, 578 (1988).
Hay, B., Ackerman, L., Barbel, S., Jan, L. Y. & Jan, Y. N. Development 103, 625–640 (1988).
Lane, D. P. & Hoeffler, W. K. Nature 288, 167–170 (1980).
Rhoads, R. E. Trends biochem. Sci. 13, 52–56 (1988).
Pain, V. M. Biochem. J. 235, 625–637 (1986).
Abramson, R. D. et al. J. biol. Chem. 262, 3826–3832 (1987).
Abramson, R. D. et al. J. biol. Chem. 263, 5462–5467 (1988).
Ray, B. K. et al. J. biol. Chem. 260, 7651–7658 (1985).
Lawson, T. G. et al. J. biol. Chem. 261, 13979–13989 (1986).
Nielsen, P. J. & Trachsel, H. EMBO J. 7, 2097–2105 (1988).
Gubb, D., Shelton, M., Roote, J., McGill, S. & Ashburner, M. Chromosoma 91, 54–64 (1984).
Ashburner, M., Aaron, P. S. & Tsubota, S. Genetics 102, 421–435 (1982).
Pardue, M. L. in Nucleic Acid Hybridisation: A Practical Approach 179–202 (IRL, Oxford, 1985).
Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).
Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 137, 266–267 (1984).
Chia, W., Savakis, C., Karp, R., Pelham, H. & Ashburner, M. J. molec. Biol. 186, 679–688 (1985).
Fyrberg, E. A., Bond, B. J., Hershey, N. D., Mixter, K. S. & Davidson, N. Cell 24, 107–116 (1981).
Akam, M. E. & Martinez-Arias, A. EMBO J. 4, 1689–1700 (1985).
Sánchez-Herrero, E. & Crosby, M. A. EMBO J. 7, 2163–2173 (1988).
Ingham, P. W., Howard, K. R. & Ish-Horowicz, D. Nature 318, 439–445 (1985).
Campos-Ortega, J. A. & Hartenstein, V. The Embryonic Development of Drosophila melanogaster (Springer, Berlin, 1985).
Messing, J. Meth. Enzym. 101, 20 (1983).
Barnes, W. M., Bevan, M. & Son, P. H. Meth. Enzym. 101, 98–122 (1983).
Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).
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Lasko, P., Ashburner, M. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A. Nature 335, 611–617 (1988). https://doi.org/10.1038/335611a0
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DOI: https://doi.org/10.1038/335611a0
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