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Lymphocyte activation by HIV-1 envelope glycoprotein

Abstract

Cell activation by phytohaemagglutinin, phorbol ester and by the supernatant of phytohaemagglutinin-stimulated peripheral blood mononuclear cells induces the expression and cytopathic effects of latent human immunodeficiency virus type-1 (HIV-1) in vitro1–3. The lymphocyte surface protein CD4 has been identified as a receptor for HIV-14,5 and binds the viral envelope glycoprotein (gp120)6,7. In the light of evidence indicating that one natural function of CD4 is as a growth factor receptor8–10, we examined the ability of native gp120 to activate resting CD4-bearing lymphocytes. Our results indicate that gp120 has innate biological activity as a result of a specific interaction with CD4, inducing increases in intracellular levels of inositol trisphosphate and of calcium, and in interleukin-2 receptor expression and cell motility.

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References

  1. 1

    Zagury, D. et al. Science 231, 850–853 (1986).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Harada, S., Koyanagi, Y., Nakashima, H., Kobayashi, N. & Yamamoto, N. Virology 154, 249–258 (1986).

    CAS  Article  Google Scholar 

  3. 3

    Folks, T. M., Justement, J., Kinter, A., Dinarello, C. A. & Fauci, A. S. Science 238, 800–802 (1987).

    ADS  CAS  Article  Google Scholar 

  4. 4

    McDougal, J. S. et al. Science 231, 382–385 (1986).

    ADS  CAS  Article  Google Scholar 

  5. 5

    Maddon, P. J. et al. Cell 47, 333–348 (1986).

    CAS  Article  Google Scholar 

  6. 6

    Kowalski, M. et al. Science 237, 1351–1355 (1987).

    ADS  CAS  Article  Google Scholar 

  7. 7

    Lasky, L. A. et al. Cell 50, 975–985 (1987).

    CAS  Article  Google Scholar 

  8. 8

    Acres, R. B., Conlon, P. J., Mochizuki, D. Y. & Gallis, B. J. Immun. 261, 16210–16214 (1986).

    CAS  Google Scholar 

  9. 9

    Liftman, D. Annls. Inst. Pasteur, Pans 138, 147–150 (1987).

    Article  Google Scholar 

  10. 10

    Cruikshank, W. W., Berman, J. S., Theodore, A. C, Bernardo, J. & Center, D. M. J. Immun. 138, 3817–3823 (1987).

    CAS  PubMed  Google Scholar 

  11. 11

    Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. Proc. natn. Acad. Sci. U.S.A. 76, 4061–4065 (1979).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Reinherz, E. L. & Schlossman, S. F. Cell 19, 821–827 (1980).

    CAS  Article  Google Scholar 

  13. 13

    Greenstein, J. L., Kappler, J., Marrack, P. & Burakoff, S. J. J. exp. Med. 159, 1213–1224 (1984).

    CAS  Article  Google Scholar 

  14. 14

    Gay, D., Coeshott, C., Golde, W., Kappler, J. & Marrack, P. J. Immun. 136, 2026 (1986).

    CAS  PubMed  Google Scholar 

  15. 15

    Doyle, C. & Strominger, J. L. Nature 330, 256–259 (1987).

    ADS  CAS  Article  Google Scholar 

  16. 16

    Gay, D. et al. Nature 328, 626–629 (1987).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Kaye, J., Porcelli, S., Tite, J., Jones, B. & Janeway, C. A. J. exp. Med. 158, 836–856 (1983).

    CAS  Article  Google Scholar 

  18. 18

    Kaye, J. & Janeway, C. A. J. exp. Med. 159, 1397–1412 (1984).

    CAS  Article  Google Scholar 

  19. 19

    Kumagai, N., Benedict, S. H., Mills, G. B. & Gelfand, E. W. J. Immun. 140, 37–43 (1988).

    CAS  PubMed  Google Scholar 

  20. 20

    Kupfer, A., Singer, S. J., Janeway, C. A. & Swain, S. L. Proc. naln. Acad. Sci. U.S.A. 84, 5888–5892 (1987).

    ADS  CAS  Article  Google Scholar 

  21. 21

    Maddon, P. J. et al. Cell 42, 93–104 (1985).

    CAS  Article  Google Scholar 

  22. 22

    Blue, M.-L., Hafler, D. A., Craig, K. A., Levine, H. & Schlossman, S. F. J. Immun. 139, 3949–3954 (1987).

    CAS  PubMed  Google Scholar 

  23. 23

    Hoxie, J. A., Rackowski, J. L., Haggarty, B. S. & Gaulton, G. N. J. Immun. 140, 786–795 (1988).

    CAS  PubMed  Google Scholar 

  24. 24

    O'Flynn, K., Linch, D. C. & Tatham, P. E. R. Biochem. J. 219, 661–666 (1984).

    CAS  Article  Google Scholar 

  25. 25

    Imboden, J. B., Weiss, A. & Stobo, J. D. Immun. Today 6, 328–331 (1985).

    CAS  Article  Google Scholar 

  26. 26

    Harris, D. T., Kozumbo, W. J., Cerutti, P. A. & Cerottini, J.-C. J. Immun. 138, 600–605 (1987).

    CAS  PubMed  Google Scholar 

  27. 27

    Kozumbo, W. J., Harris, D. T., Gromkowski, S., Cerottini, J.-C. & Cerutti, P. A. J. Immun. 138, 606–612 (1987).

    CAS  PubMed  Google Scholar 

  28. 28

    Imboden, J. B., Weyand, C. & Goronzy, J. J. Immun. 138, 1322–1344 (1987).

    CAS  PubMed  Google Scholar 

  29. 29

    Rosoff, P. M., Burakoff, S. J. & Greenstein, J. L. Cell 49, 845–853 (1987).

    CAS  Article  Google Scholar 

  30. 30

    Pyle, S. W. et al. AIDS Res. Hum. Retrovir. 3, 387–400 (1988).

    Article  Google Scholar 

  31. 31

    Robey, W. G. et al. Proc. natn. Acad. Sci. U.S.A. 83, 7023–7027 (1986).

    ADS  CAS  Article  Google Scholar 

  32. 32

    Sattentau, Q. J., Dalgleish, A. G., Weiss, R. A. & Beverley, P. C. Science 234, 1120–1123 (1986).

    ADS  CAS  Article  Google Scholar 

  33. 33

    McDougal, J. S. et al. J. Immun. 137, 2937–2944 (1986).

    CAS  PubMed  Google Scholar 

  34. 34

    Grynkiewicz, G., Poenie, M. & Tsien, R. Y. J. bio. Chem. 260, 3440–3450 (1985).

    CAS  Google Scholar 

  35. 35

    Kristensen, C. W., Bettens, F. & DeWeck, A. L. J. Immun. 130, 1770–1733 (1983).

    PubMed  Google Scholar 

  36. 36

    Kornfeld, H., Berman, J. S. Beer, D. J. & Center, D. M. J. Immun. 134, 3887–3890 (1985).

    CAS  PubMed  Google Scholar 

  37. 37

    Berman, J. S. & Center, D. M. J. Immun. 138, 2100–2103 (1987).

    CAS  PubMed  Google Scholar 

  38. 38

    Tapson, V. F., Boni-Schnetzler, M., Pilch, P. F., Center, D. M. & Berman, J. S. J. clin. Invest. (in the press).

  39. 39

    Center, D. M. & Cruikshank, W. J. Immun. 128, 2563–2568 (1982).

    CAS  PubMed  Google Scholar 

  40. 40

    Zigmond, S. H. & Hirsch, J. G. J. exp. Med. 137, 387–410 (1973).

    CAS  Article  Google Scholar 

  41. 41

    Gelderblom, H. R., Reupke, H. & Pauli, G. Lancet ii, 1016–1017 (1985).

    Article  Google Scholar 

  42. 42

    Schneider, J., Kaaden, O., Copeland, T. D., Oroszlan, S. & Hunsmann, G. J. gen. Virol 67, 2533–2538 (1986).

    CAS  Article  Google Scholar 

  43. 43

    Gelderblom, H. R., Hausmann, H. S., Ozel, M., Pauli, G. & Koch, M. A. Virology 156, 171–176 (1987).

    CAS  Article  Google Scholar 

  44. 44

    Gartner, S. et al. Science 233, 215–218 (1986).

    ADS  CAS  Article  Google Scholar 

  45. 45

    Nabel, G. & Baltimore, D. Nature 326, 711–713 (1987).

    ADS  CAS  Article  Google Scholar 

  46. 46

    Siekevitz, M. et al. Science 238, 1575–1578 (1987).

    ADS  CAS  Article  Google Scholar 

  47. 47

    Center, D. M., Cruikshank, W. W. Berman, J. S. & Beer, D. J. J. Immun. 131, 1854–1859 (1983).

    CAS  PubMed  Google Scholar 

  48. 48

    Imboden, J. B. & Stobo, J. D. J. exp. Med. 161, 446–456 (1985).

    CAS  Article  Google Scholar 

  49. 49

    Wilson, D. B. et al. J. biol. Chem. 260, 13496–13501 (1985).

    CAS  PubMed  Google Scholar 

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Kornfeld, H., Cruikshank, W., Pyle, S. et al. Lymphocyte activation by HIV-1 envelope glycoprotein. Nature 335, 445–448 (1988). https://doi.org/10.1038/335445a0

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