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Membrane potential has no direct role in evoking neurotransmitter release

Abstract

Neurons communicate by secreting a transmitter that excites or inhibits other neurons at synapses. The role of presynaptic membrane potential in triggering transmitter release is still controversial. In one view, presynaptic action potentials trigger the release by the entry of calcium ions into presynaptic terminals through voltage-dependent calcium channels1. Calcium acts at high local concentrations at release sites near channel mouths to cause neurosecretion2–5. An opposing view is that, in addition to elevating presynaptic calcium, presynaptic potential stimulates transmitter release by a distinct direct action1,6–15. The relative importance of depolarization and calcium entry in neurosecretion cannot be determined because the two events are tightly linked16–20. To delineate the roles of presynaptic potential and calcium entry in transmitter release, we have used nitr-5, a photolabile calcium chelator21–22, and a voltage-clamp technique to control intracellular calcium and membrane potential independently at a synapse formed between cell bodies of cultured neurons of the fresh water snail Helisoma trivolvis. We found transmitter release occurred when presynaptic calcium levels were elevated to concentrations of a few micromolar, and that presynaptic voltage had no direct effect on neurosecretion.

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Zucker, R., Haydon, P. Membrane potential has no direct role in evoking neurotransmitter release. Nature 335, 360–362 (1988). https://doi.org/10.1038/335360a0

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