Abstract
In both starfish and amphibian oocytes, the activity of a major protein kinase which is independent of Ca2+ and cyclic nucleotides increases dramatically at meiotic and mitotic nuclear divisions1–6. The in vivo substrates of this kinase are unknown, but phosphorylation of H1 histone can be used as an in vitro assay. We have purified this kinase from starfish oocytes. The major band in the most highly purified preparation contained a polypeptide of relative molecular mass (Mr) 34,000 (34K). This is the same size as the protein kinase encoded by cdc2+, which regulates entry into mitosis in fission yeast7–11 and is a component of MPF purified from Xenopus12. Here, we show that antibodies against p34 recognize the starfish 34K protein and propose that entry into meiotic and mitotic nuclear divisions involves activation of the protein kinase encoded by a homologue of cdc2+. Given the wide occurrence of cdc2+ homologues from budding yeast to Xenopus and human cells9,11,12, this activation may act as a common mechanism controlling entry into mitosis in eukaryotic cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Capony, J. P., Picard, A., Peaucellier, G., Labbe, J. C. & Doree, M. Devl Biol. 117, 1–12 (1986).
Guerrier, P., Moreau, M. & Doree, M. Molec. cell. Endocrin. 7, 137–150 (1977).
Sano, K. Dev. Growth Different. 27, 262–275 (1985).
Picard, A. et al. Dev. Growth Different. 29, 93–103 (1987).
Labbe, J. C., Picard, A., Karsenti, E. & Doree, M. Devl Biol. (in the press).
Dabauvalle, M. C., Doree, M., Bravo, R. & Karsenti, E. Cell 52, 525–533 (1988).
Simanis, V. & Nurse, P. Cell 45, 261–268 (1986).
Reed, S. I., Hadwiger, J. A. & Lorincz, A. T. Proc. natn. Acad. Sci. U.S.A. 82, 4055–4059 (1985).
Lee, M. G. & Nurse, P. Nature 327, 31–35 (1987).
Beach, D., Durkacz, B. & Nurse, P. Nature 300, 706–709 (1982).
Draetta, G., Brizuela, L., Potashkin, J. & Beach, D. Cell 50, 319–325 (1987).
Gautier, J., Norbury, C., Lohka, M., Nurse, P. & Maller, J. Cell 54, 433–439 (1988).
Kanatani, H., Shirai, H., Nakanishi, K. & Kurokawa, T. Nature 211, 273–277 (1969).
Picard, A., Peaucellier, G., Le Bouffant, F., Le Peuch, C. & Doree, M. Devl Biol. 109, 311–320 (1985).
Pelech, S. L., Meijer, L. & Krebs, E. G. Biochemistry 26, 7960–7968 (1987).
Russell, P. & Nurse, P. Cell 49, 559–567 (1987).
Russell, P. & Nurse, P. Cell 49, 469–576 (1987).
Bradbury, E., Inglis, R. & Matthews, H. Nature 247, 257–261 (1974).
Witts, J. J. & Roskowski, R. Jr Analyt. Biochem. 66, 253–258 (1975).
Tasheva, B. & Dessev, G. Analyt. Biochem. 60, 98–102 (1983).
Quirin-Stricker, C. Eur. J. Biochem. 142, 317–322 (1984).
Draetla, G. & Beach, D. Cell 54, 17–26 (1988).
Dunphy, W., Brizuela, L., Beach, D. & Newport, J. Cell 54, 423–431 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Labbe, J., Lee, M., Nurse, P. et al. Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+. Nature 335, 251–254 (1988). https://doi.org/10.1038/335251a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/335251a0
This article is cited by
-
Cyclins and cyclin-dependent kinases: from biology to tumorigenesis and therapeutic opportunities
Journal of Cancer Research and Clinical Oncology (2023)
-
Requirement for CCNB1 in mouse spermatogenesis
Cell Death & Disease (2017)
-
New Cdc2 Tyr 4 phosphorylation by dsRNA‐activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses
EMBO reports (2010)
-
Identification and characterization of maturation-promoting factor from catfish,Clarias batrachus
Fish Physiology and Biochemistry (1996)
-
Presence of maturation-promoting factor in 17?,20?-dihydroxy-4-pregnen-3-one-induced oocytes of catfish, Clarias batrachus
Fish Physiology and Biochemistry (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.