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Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes


Regulation of expression of specific genes by antisense RNA is a naturally occurring mechanism in bacteria1,2, although gene regulation by this mechanism has not yet been observed in higher eukaryotes. However, antisense RNA has been shown to reduce expression of specific genes when injected into frog oocytes3 and Drosophila embryos4. Inhibition of expression of artificially introduced genes has been demonstrated by transient expression of antisense RNA constructs in mammalian cells5,6, and plant protoplasts7, and by stable expression in transgenic plants8. Here, we report a striking inhibition of expression of the endogenous, developmentally regulated gene for polygalacturonase in stably transformed tomato expressing antisense RNA.

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  1. Minzuno, T., Chou, M. & Inouye, M. Proc. natn. Acad. Sci. U.S.A. 81, 1966–1970 (1984).

    Article  ADS  Google Scholar 

  2. Simons, R. W. & Kleckner, N. Cell 34, 683–691 (1983).

    Article  CAS  Google Scholar 

  3. Melton, D. A. Proc. natn. Acad. Sci. U.S.A. 82, 144–148 (1985).

    Article  ADS  CAS  Google Scholar 

  4. Rosenberg, U. B., Preiss, A., Seifert, E., Jackle, H. & Knipple, D. C. Nature 313, 703–70 (1985).

    Article  ADS  CAS  Google Scholar 

  5. Izant, J. C. & Weintraub, H. Cell 36, 1007–1015 (1984).

    Article  CAS  Google Scholar 

  6. Kim, S. K. & Wold, B. J. Cell 42, 129–138 (1985).

    Article  CAS  Google Scholar 

  7. Ecker, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 83, 5372–5376 (1986).

    Article  ADS  CAS  Google Scholar 

  8. Rothstein, S. J., DiMaio, J., Strand, M. & Rice, D. Proc. natn. Acad. Sci. U.S.A. 84, 8439–8443 (1987).

    Article  ADS  CAS  Google Scholar 

  9. Tucker, G. A., Robertson, N. E. & Grierson, D. Eur. J. Biochem. 112, 119–124 (1980).

    Article  CAS  Google Scholar 

  10. Themmen, A. P. N., Tucker, G. A. & Grierson, D. Plant Physiol. 69, 122–124 (1982).

    Article  CAS  Google Scholar 

  11. Crookes, P. R. & Grierson, D. Pl. Physiol. 72, 1088–1093 (1983).

    Article  CAS  Google Scholar 

  12. Tucker, G. A. & Grierson, D. Planta 155, 64–67 (1982).

    Article  CAS  Google Scholar 

  13. Grierson, D. et al. Phil. Trans. R. Soc. B 399–410 (1986).

  14. Dellapenna, D., Alexander, D. C. & Bennett, A. B. Proc. natn. Acad. Sci. U.S.A 83, 6420–6424 (1986).

    Article  ADS  CAS  Google Scholar 

  15. Biggs, M. S., Harriman, R. W. & Handa, A. K. Pl. Physiol. 81, 395–403 (1986).

    Article  CAS  Google Scholar 

  16. Grierson, D. et al. Nucleic Acids Res. 14, 8595–8603 (1986).

    Article  CAS  Google Scholar 

  17. Bird, C. R. et al. Pl. molec. Biol. (submitted).

  18. Tucker, G. A., Robertson, N. G. & Grierson, D. Eur. J. Biochem. 115, 87–90 (1981).

    Article  CAS  Google Scholar 

  19. Ali, Z. M. & Brady, C. J. Aust. J. Pl. Physiol. 9, 155–169 (1982).

    CAS  Google Scholar 

  20. Pressey, R. HortScience 21, 1183–1185 (1986).

    CAS  Google Scholar 

  21. Grierson, D. Oxford Surveys Pl. molec. cell Biol. 3, 364–383 (1986).

    Google Scholar 

  22. Dellapenna, D. & Bennett, A. B. Pl. Physiol. (in the press, 1988).

  23. Presssey, R. Eur. J. Biochem. 144, 217–221 (1984).

    Article  Google Scholar 

  24. Guilley, H., Dudley, R. K., Jonard, G., Balazs, E. & Richards, K. E. Cell 30, 763–773 (1982).

    Article  CAS  Google Scholar 

  25. Depicker, A., Stachel, S., Dhaese, P., Zambryski, P. & Goodman, H. M. J. molec. appl. Genet. 1, 561–573 (1982).

    CAS  Google Scholar 

  26. Bevan, M. W. Nucleic Acids Res. 12, 8711–8721 (1984).

    Article  CAS  Google Scholar 

  27. Franck, G., Guille, M., Jonard, G., Richards, K. & Hirth, L. Cell 21, 285–294 (1980).

    Article  CAS  Google Scholar 

  28. Raeder, U. & Broda, P. Lett. appl. Microbiol. 1, 17–20 (1985).

    Article  CAS  Google Scholar 

  29. Chomczynski, P. & Qasba, P. K. Biochem. biophys. Res. Commun. 122, 340–344 (1984).

    Article  CAS  Google Scholar 

  30. Grierson, D., Slater, A., Speirs, J. & Tucker, G. A. Planta 163, 263–277 (1985).

    Article  CAS  Google Scholar 

  31. Smith, C. J. S., Slater, A. & Grierson, D. Planta 168, 94–100 (1986).

    Article  CAS  Google Scholar 

  32. Tomes, M. L. Bot. Gaz. 124, 180–185 (1963).

    Article  CAS  Google Scholar 

  33. Seymour, G. B., Harding, S. E., Taylor, A. J., Hobson, G. E. & Tucker, G. A. Phytochemistry 26, 1871–1875 (1987).

    Article  CAS  Google Scholar 

  34. van der Krol, A. R. et al. Nature 333, 866–869 (1988).

    Article  ADS  CAS  Google Scholar 

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Smith, C., Watson, C., Ray, J. et al. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724–726 (1988).

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