Abstract
Protein 4.9, first identified as a component of the human erythrocyte membrane skeleton, binds to and bundles actin filaments1. Protein 4.9 is a substrate for various kinases, including a cyclic AMP(cAMP)-dependent one, in vivo and in vitro2–7. We show here that phosphorylation of protein 4.9 by the catalytic subunit of cAMP-dependent protein kinase reversibly abolishes its actin-bundling activity, but phosphorylation by protein kinase C has no such effect. A quantitative immunoassay showed that human erythrocytes contain 43,000 trimers of protein 4.9 per cell, which is equivalent to one trimer for each actin oligomer in these red blood cells. As analogues of protein 4.9 have been identified together with analogues of other erythroid skeletal proteins in non-erythroid tissues of numerous vertebrates8, phosphorylation and dephosphorylation of protein 4.9 may be the basis for a mechanism that regulates actin bundling in many cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Siegel, D. L. & Branton, D. J. Cell Biol. 100, 7757–785 (1985).
Ling, E. & Sapirstein, V. Biochem. biophys. Res. Commun. 120, 291–298 (1984).
Home, W. C., Leto, T. L. & Marchesi, V. T. J. biol. Chem. 260, 9073–9076 (1985).
Palfrey, H. C. & Waseem, A. J. biol. Chem. 260, 16021–16029 (1985).
Faquin, W. C., Chahwala, S. B., Cantley, L. C. & Branton, D. Biochim. biophys. Acta 887, 142–149 (1986).
Cohen, C. M. & Foley, S. F. J. biol. Chem. 261, 7701–7709 (1986).
Husain, A. & Branton, D. Analyt. Biochem. 155, 206–211 (1986).
Faquin, W. C, Husain, A., Hung, J. & Branton, D. Eur. J. Cell Biol. 46, 168–175 (1988).
Pollard, T. D. & Cooper, J. A. A. Rev. Biochem. 55, 987–1035 (1986).
Byers, T. J. & Branton, D. Proc. natn. Acad. Sci. U.S.A. 82, 6153–6157 (1985).
Pinder, J. C. & Gratzer, W. B. J. Cell Biol. 96, 768–775 (1983).
Elgsaeter, A., Stokke, B. T., Mikkelsen, A. & Branton, D. Science 234, 1217–1223 (1986).
Craig, S. W. & Pollard, T. D. Trends biochem. Sci. 7, 88–92 (1982).
Bahler, M. & Greenhard, P. Nature 326, 704–707 (1987).
Baines, A. J. & Bennett, V. Nature 315, 410–413 (1985).
Cohen, C. M., Foley, S. F. & Korgsgren, C. Nature 299, 648–650 (1982).
Spiegel, J. E., Beardsley, D. S., Southwick, F. S. & Lux, S. E. J. Cell Biol. 99, 886–893 (1984).
Ungewickell, E., Bennett, P., Calvert, R., Ohanian, V. & Gratzer, W. Nature 280, 811–814 (1979).
Fowler, V. & Taylor, D. L. J. Cell Biol. 85, 361–376 (1980).
DeCamilli, P., Harris, J. M. Jr., Hunter, W. B. & Greengard, P. J. Cell Biol. 96, 1355–1373 (1983).
Huttner, W. B., Schiebler, W. & Greengard, P. J. Cell Biol. 96, 1374–1388 (1983).
Laemmli, U. K. Nature 227, 680–685 (1970).
Malloy, J. M., Rieker, J. P. & Rizzo, C. F. Analyt. Biochem. 141, 503–509 (1984).
Ingebritsen, T. S. & Cohen, P. Science 221, 331–338 (1983).
Cuello, A. C., Milstein, C. & Galfre, G. in Immunohistochemistry (ed. Cuello, A. C.) 215–256 (John Wiley & Sons, New York 1983).
Jaenicke, L. Analyt. Biochem. 61, 623–627 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Husain-Chishti, A., Levin, A. & Branton, D. Abolition of actin-bundling by phosphorylation of human erythrocyte protein 4.9. Nature 334, 718–721 (1988). https://doi.org/10.1038/334718a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/334718a0
This article is cited by
-
Hypermethylation of DMTN promotes the metastasis of colorectal cancer cells by regulating the actin cytoskeleton through Rac1 signaling activation
Journal of Experimental & Clinical Cancer Research (2018)
-
abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing
Cell Discovery (2018)
-
Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation
Clinical Proteomics (2013)
-
Reversible erythrocyte skeleton destabilization is modulated by beta-spectrin phosphorylation in childhood leukemia
Leukemia (2001)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
