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Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the Fungi

Nature volume 334, pages 519–522 (1988)Cite this article

Abstract

Pneumocystis carinii pneumonia is the most common opportunistic infection in AIDS1, and accounts for significant morbidity and mortality in these and other immunocompromised patients2. P. carinii is a eukaryotic microorganism of uncertain taxonomy that can infect numerous mammalian hosts. Developing from a small, unicellular 'trophozoite' into a 'cyst' containing eight 'sporozoites', its life cycle superficially resembles those seen both in the Protozoa and Fungi. Morphological and ultrastructural observations have lead some investigators to conclude that the organism is a protozoan3–6, while others have felt that it more closely resembles a fungus7–9. Phylogenetic relationships can be inferred from comparisons of macromolecular sequences10. Small subunit ribosomal RNAs (16S-like rRNAs) are well-suited for this purpose because they have the same function in all organisms and contain sufficient information to estimate both close and distant evolutionary relationships. Phylogenetic frameworks based upon such comparisons reveal that the plant, animal and fungal lineages are distinct from the diverse spectrum of protozoan lineages11. In this letter, phylogenetic analysis of Pneumocystis 16S-like rRNA demonstrates it to be a member of the Fungi.

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Authors and Affiliations

  1. Hormone Research Institute, University of California, San Francisco, California, 94143-0534, USA

    Jeffrey C. Edman

  2. Department of Laboratory Medicine, University of California, San Francisco, California, 94143-0534, USA

    Jeffrey C. Edman & Daniel V. Santi

  3. Departments of Biochemistry and Biophysics, University of California, San Francisco, California, 94143-0534, USA

    Daniel V. Santi

  4. Department of Pharmaceutical Chemistry, University of California, San Francisco, California, 94143-0534, USA

    Daniel V. Santi

  5. Department of Critical Care Medicine, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Joseph A. Kovacs & Henry Masur

  6. Division of Molecular and Cellular Biology, Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, 80206, USA

    Hille J. Elwood & Mitchell L. Sogin

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  2. Joseph A. Kovacs
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  5. Hille J. Elwood
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  6. Mitchell L. Sogin
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Edman, J., Kovacs, J., Masur, H. et al. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the Fungi. Nature 334, 519–522 (1988). https://doi.org/10.1038/334519a0

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