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A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs

Abstract

The CTF/NF-I group of cellular DNA binding proteins recognizes the sequence GCCAAT and is implicated in eukaryotic transcription as well as DNA replication. Molecular analysis of human CTF/NF-I complementary DNA clones reveals multiple messenger RNA species containing alternative coding regions, apparently as a result of differential splicing. Expression and functional analysis establish that individual gene products can bind to GCCAAT recognition sites and serve both as promoter-selective transcriptional activators and as initiation factors for DNA replication.

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References

  1. Pruijn, G. J. M., Van Driel, W. & van der Vliet, P. C. Nature 322, 656–659 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Jones, K. A., Kadonaga, J. T., Rosenfeld, P. J., Kelly, T. J. & Tjian, R. Cell 48, 79–89 (1987).

    Article  CAS  PubMed  Google Scholar 

  3. Benoist, C. O'Hare, K., Breathnach, R. & Chambon, P. Nucleic Acids Res. 8, 127–142 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Efstradiadis, A. et al. Cell 21, 653–668 (1980).

    Article  Google Scholar 

  5. McKnight, S. L., Kingsbury, R. C., Spence, A. & Smith, M. Cell 37, 253–262 (1984).

    Article  CAS  PubMed  Google Scholar 

  6. Siebenlist, U., Hennighausen, L., Battey, J. & Leder, P. Cell 37, 381–391 (1984).

    Article  CAS  PubMed  Google Scholar 

  7. Hennighausen, L. et al. Nature 314, 289–292 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Nowock, J., Borgmeyer, U., Püschel, A. W., Rupp, R. A. & Sippel, A. E. Nucleic Acids Res. 13, 2045–2061 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Bienz, M. & Pelham, H. R. B. Cell 45, 753–760 (1986).

    Article  CAS  PubMed  Google Scholar 

  10. Graves, B. J., Johnson, P. F. & McKnight, S. L. Cell 44, 565–572 (1986).

    Article  CAS  PubMed  Google Scholar 

  11. Theisen, M., Stief, A. & Sippel, A. E. EMBO J. 5, 719–724 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Cereghini, S., Raymondjean, M., Carranca, A. G., Herbomel, P. & Yaniv, M. Cell 50, 627–638 (1987).

    Article  CAS  PubMed  Google Scholar 

  13. Morgan, W. D. et al. Molec. cell. Biol. 7, 1129–1138 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Jones, K. A., Yamamoto, K. R. & Tjian, R. Cell 42, 559–572 (1985).

    Article  CAS  PubMed  Google Scholar 

  15. Nagata, K., Guggenheimer, R. A. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 80, 6177–6181 (1983).

    Article  ADS  CAS  Google Scholar 

  16. Rawlins, D. R., Rosenfeld, P. J., Wides, R. J., Challberg, M. D. & Kelly, T. J. Jr, Cell 37, 309–319 (1984).

    Article  CAS  PubMed  Google Scholar 

  17. Guggenheimer, R. A., Stillman, B. W., Nagata, K., Tamanoi, F. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 81, 3069–3073 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Leegwater, P. A. J., Van Driel, W. & van der Vliet, P. C. EMBO J. 4, 1515–1521 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Rosenfeld, P. J. & Kelly, T. J. J. biol. Chem. 261, 1398–1408 (1986).

    CAS  PubMed  Google Scholar 

  20. Liebhaber, S. A., Gossens, M. J. & Kan, Y. W. Proc. natn. Acad. Sci. U.S.A. 77, 7054–7058 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Ishii, S., Merlino, G. T. & Pastan, I. Science 230, 1378–1381 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Benyajati, C., Spoerel, N., Haymerle, H., & Ashburner, M. Cell 33, 125–133 (1983).

    Article  CAS  PubMed  Google Scholar 

  23. Rosenfeld, P. J., O'Neill, E. A., Wides, R. J. & Kelly, T. J. Molec. cell. Biol. 7, 875–886 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Olesen, J., Hahn, S. & Guarente, L. Cell 51, 953–961 (1987).

    Article  CAS  PubMed  Google Scholar 

  25. Chodosh, L. A., Baldwin, A. S., Carthew, R. W. & Sharp, P. A. Cell 53, 11–24 (1988).

    Article  CAS  PubMed  Google Scholar 

  26. Chodosh, L. A. et al. Cell 53, 25–35 (1988).

    Article  CAS  PubMed  Google Scholar 

  27. Shore, D. & Nasmyth, K. Cell 51, 721–732 (1987).

    Article  CAS  PubMed  Google Scholar 

  28. Brand, A. H., Micklem, G. & Nasmyth, K. Cell 51, 709–719 (1987).

    Article  CAS  PubMed  Google Scholar 

  29. Dorn, A., Bollekens, J., Staub, A., Benoist, C. & Mathis, D. Cell 50, 863–872 (1987).

    Article  CAS  PubMed  Google Scholar 

  30. Johnson, P. F., Landschulz, W. H., Graves, B. J. & McKnight, S. L. Genes Dev. 1, 133–146 (1987).

    Article  CAS  PubMed  Google Scholar 

  31. Lichtsteiner, S., Wuarin, J. & Schibler, U. Cell 50, 963–973 (1987).

    Article  Google Scholar 

  32. Miksicek, R., Borgmeyer, U. & Nowock, J. EMBO J. 6, 1355–1360 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Oikarinen, J., Hatamochi, A. & deCrombrugghe, B. J. biol. Chem. 262, 11064–11070 (1987).

    CAS  PubMed  Google Scholar 

  34. Raymondjean, M., Cereghini, S. & Yaniv, M. Proc. natn. Acad. Sci. U.S.A. 85, 757–761 (1988).

    Article  ADS  CAS  Google Scholar 

  35. Landschulz, B. & McKnight, S. Genes Dev. (in the press).

  36. Salzman, N. P., Venkatachala, N. & Selzer, G. B. in The Papovaviridae Vol. 1. The Polyomaviruses (ed. Salzman, N. P.) 27–98 (Plenum, New York, 1986).

    Google Scholar 

  37. Veldman, G. M., Lupton, S. & Kamen, R. Molec. cell. Biol. 5, 649–658 (1987).

    Article  Google Scholar 

  38. Stenlund, A., Bream, G. L. & Botchan, M. R. Science 236, 1666–1671 (1985).

    Article  ADS  Google Scholar 

  39. Reisman, D. & Sugden, B. Molec. cell. Biol. 6, 3838–3846 (1988).

    Article  Google Scholar 

  40. DePamphilis, M. L. Cell 52, 635–638 (1988).

    Article  CAS  PubMed  Google Scholar 

  41. Rüegg, U. T. & Rudinger, J. in Methods in Enzymology Vol. 47 (eds Hirs, C. H. W. & Timasheff, S. N.) 111–116 (Academic, New York, 1977).

    Google Scholar 

  42. Andrews, P. C. & Dixon, J. E. Anal. Biochem. 161, 524–528 (1987).

    Article  CAS  PubMed  Google Scholar 

  43. Lathe, R. J. molec. Biol. 183, 1–12 (1985).

    Article  CAS  PubMed  Google Scholar 

  44. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  45. Hauser, C. A. et al. Cell 43, 19–28 (1985).

    Article  CAS  PubMed  Google Scholar 

  46. Santoro, C. et al. Nucleic Acids Res. 14, 2863–2876 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Kadonaga, J. T., Carner, K. R., Masiarz, F. R. & Tjian, R. Cell 51, 1079–1090 (1988).

    Article  Google Scholar 

  48. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  49. Gross, C., Engbaek, F., Flamang, T. & Burgess, R. J. Bact. 128, 382–389 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Kadonaga, J. T. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 83, 5889–5893 (1986).

    Article  ADS  CAS  Google Scholar 

  51. Mellon, P., Parker, V., Gluzman, Y. & Maniatis, T. Cell 27, 279–288 (1981).

    Article  CAS  PubMed  Google Scholar 

  52. Luckow, B. & Schütz, G. Nucleic Acids Res. 15, 5490 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. DiNocera, P. D. & Dawid, I. B. Proc. natn. Acad. Sci. U.S.A. 80, 7095–7098 (1983).

    Article  ADS  CAS  Google Scholar 

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Santoro, C., Mermod, N., Andrews, P. et al. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs. Nature 334, 218–224 (1988). https://doi.org/10.1038/334218a0

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