Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Identification of a second human retinoic acid receptor

Nature volume 332, pages 850–853 (1988)Cite this article

Abstract

We have previously described a human complementary DNA that encodes a novel protein which is homologous to members of the steroid/thyroid nuclear receptor multigene family1. This novel protein (hap for hepatoma) exhibits strong homology with the human retinoic acid receptor (RAR)1,2 which has been recently characterized2,3. To test the possibility that the hap protein might also be a retinoid receptor, a chimaeric receptor was created by replacing the putative DNA binding domain of hap with that of the human oestrogen receptor (ER). The resulting hap-ER chimaera was then tested for its ability to trans-activate an oestrogen-responsive reporter gene (vit–tk–CAT) in the presence of possible receptor ligands. Here we show that retinoic acid (RA) at physiological concentrations is effective in inducing the expression of this reporter gene by the hap-ER chimaeric receptor. This demonstrates the existence of two human retinoic acid receptors designated RAR-α and RAR-β.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

References

  1. de Thé, H., Marchio, A., Tiollais, P. & Dejean, A. Nature 330, 667–670 (1987).

    Article  ADS  Google Scholar 

  2. Petkovich, M., Brand, N. J., Krust, A. & Chambon, P. Nature 330, 444–450 (1987).

    CAS  Article  ADS  Google Scholar 

  3. Giguere, V., Ong, E. S., Segui, P. & Evans, R. M. Nature 330, 624–629 (1987).

    CAS  Article  ADS  PubMed Central  Google Scholar 

  4. Krust, A. et al. EMBO J. 5, 891–897 (1986).

    CAS  Article  PubMed Central  Google Scholar 

  5. Green, S. & Chambon, P. Nature 324, 615–617 (1986).

    CAS  Article  ADS  PubMed Central  Google Scholar 

  6. Green, S. & Chambon, P. Nature 325, 75–78 (1987).

    CAS  Article  ADS  Google Scholar 

  7. Kumar, V. et al. Cell 51, 941–951 (1987).

    CAS  Article  PubMed Central  Google Scholar 

  8. Hollenberg, S. M., Giguere, V., Segui, P. & Evans, R. M. Cell 49, 39–46 (1987).

    CAS  Article  Google Scholar 

  9. Rusconi, S. & Yamamoto, K. R. EMBO J. 6, 1309–1315 (1987).

    CAS  Article  PubMed Central  Google Scholar 

  10. Dejean, A., Bougueleret, L., Grzeschik, K. H. & Tiollais, P. Nature 322, 70–72 (1986).

    CAS  Article  ADS  Google Scholar 

  11. Green, S., Issemann, I. & Scheer, E. Nucleic Acids Res. 16, 369 (1988).

    CAS  Article  PubMed Central  Google Scholar 

  12. Williams, J. B. & Napoli, J. L. Proc. natn. Acad. Sci. U.S.A. 82, 4658–4662 (1985).

    CAS  Article  ADS  Google Scholar 

  13. Mattei, M. G., Petkovich, M., Mattei, J. F., Brand, N. & Chambon, P. Hum. Genet. (in the press).

  14. Thompson, C. C., Weinberger, C., Lebo, R. & Evans, R. M. Science 237, 1610–1614 (1987).

    CAS  Article  ADS  PubMed Central  Google Scholar 

  15. Gareau, J. L., Houle, B., Leduc, F., Bradley, W. E. C. & Dobrovic, A. Nucleic Acids Res. 16, 1223 (1988).

    CAS  Article  PubMed Central  Google Scholar 

  16. Robertson, M. Nature 330, 420–421 (1987).

    CAS  Article  ADS  PubMed Central  Google Scholar 

  17. Sporn, M. B., Roberts, A. B. & Goodman, D. S. (eds) The Retinoids (Academic, Florida, 1984).

  18. Sporn, M. B. & Roberts, A. B. in The Retinoids Vol. 1 (eds Sporn, M. B., Roberts, A. B. & Goodman, D. S.) 235–279 (Academic, Florida, 1984).

    Book  Google Scholar 

  19. Thaller, C. & Eichele, G. Nature 327, 625–628 (1987).

    CAS  Article  ADS  Google Scholar 

Download references

Author information

Affiliations

  1. Laboratoire de Génétique Moleculaire des Eucaryotes du CNRS, Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cédex, France

    Nigel Brand, Martin Petkovich, Andrée Krust & Pierre Chambon

  2. Unité de Recombinaison et Expression Génétique, Institut Pasteur, 28 Rue du Dr. Roux, 75724, Paris, Cédex 15, France

    Hugues de Thé, Agnès Marchio, Pierre Tiollais & Anne Dejean

Authors
  1. Nigel Brand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Martin Petkovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrée Krust
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre Chambon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hugues de Thé
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Agnès Marchio
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Pierre Tiollais
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Anne Dejean
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Brand, N., Petkovich, M., Krust, A. et al. Identification of a second human retinoic acid receptor. Nature 332, 850–853 (1988). https://doi.org/10.1038/332850a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/332850a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing