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Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro

Nature volume 332, pages 557–561 (1988)Cite this article

Abstract

The simian virus 40 (SV40) transcriptional enhancer is composed of multiple cis-acting DNA sequence motifs, each individually having a two-to fourfold effect on the efficiency of transcription1–8. When various distinct cis-elements act in combination, however, a dramatic enhancement of transcription initiation often results9–11. SV40-enhancer A-domain sequences were previously shown to be important for early and late transcription in vivo. Here we report the isolation of the enhancer binding factor AP-4, which recognizes a motif in this domain. Purified AP-4 activates SV40 late transcription in vitro, and this stimulation is augmented by the addition of transcription factor AP-1 which binds to adjacent sequences in the A-domain12, suggesting coordinate action of the two factors for transcriptional enhancement. AP-1 also represses late transcription from a major in vitro start site which is poorly used in vivo, indicating that AP-1 can act as both a positive and negative regulator of SV40 late transcription. Thus by manipulating the levels of different trans-acting factors in vitro, we can recreate the pattern of SV40 late initiation observed during the viral lytic cycle in vivo.

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References

  1. Weiher, H., König, M. & Gross, P. Science 219, 626–631 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Herr, W. & Gluzman, Y. Nature 313, 711–714 (1985).

    Article  ADS  CAS  Google Scholar 

  3. Keller, J. M. & Alwine, J. C. Molec. cell. Biol. 5, 1859–1869 (1985).

    Article  CAS  Google Scholar 

  4. Firak, T. A. & Subramanian, K. N. Molec. cell. Biol. 6, 3667–3676 (1986).

    Article  CAS  Google Scholar 

  5. Herr, W. & Clarke, J. Cell 45, 461–470 (1986).

    Article  CAS  Google Scholar 

  6. Zenke, M. et al. EMBO J. 5, 387–397 (1986).

    Article  CAS  Google Scholar 

  7. Ernoult-Lange, M., Omilli, F., O'Reilly, D. R. & May, E. J. Virol. 61, 167–176 (1987).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. May, E., Omilli, F., Ernoult-Lange, M., Zenke, M. & Chambon, P. Nucleic Acids Res. 15, 2445–2461 (1987).

    Article  CAS  Google Scholar 

  9. Chiu, R., Imagawa, M., Imbra, R. J., Bockoven, J. R. & Karin, M. Nature 329, 648–651 (1987).

    Article  ADS  CAS  Google Scholar 

  10. Ondek, B., Shepard, A. & Herr, W. EMBO J., 6, 1017–1025 (1987).

    Article  CAS  Google Scholar 

  11. Schirm, S., Jiricny, J. & Schaffner, W. Genes Dev., 1, 65–74 (1987).

    Article  CAS  Google Scholar 

  12. Lee, W., Mitchell, P. & Tjian, R. Cell 49, 741–752 (1987).

    Article  CAS  Google Scholar 

  13. Davidson, I. et al. Nature 323, 544–548 (1986).

    Article  ADS  CAS  Google Scholar 

  14. Wildeman, A. G. et al. Molec. cell. Biol. 6, 2098–2105 (1986).

    Article  CAS  Google Scholar 

  15. Bohraann, D. et al. Nature 325, 268–272 (1987).

    Article  ADS  Google Scholar 

  16. Lee, W., Haslinger, A., Karin, M. & Tjian, R. Nature 325, 368–372 (1987).

    Article  ADS  CAS  Google Scholar 

  17. Rosales, R. et al. EMBO J. 6, 3015–3025 (1987).

    Article  CAS  Google Scholar 

  18. Xiao, J. H. EMBO J. 6, 3005–3013 (1987).

    Article  CAS  Google Scholar 

  19. Angel, P. et al. Cell 49, 729–739 (1987).

    Article  CAS  Google Scholar 

  20. Imagawa, M., Chiu, R. & Karin, M. Cell 51, 251–260 (1987).

    Article  CAS  Google Scholar 

  21. Johnson, P. F., Landschulz, W. H., Graves, B. J. & McKnight, S. L. Genes Dev. 1, 133–146 (1987).

    Article  CAS  Google Scholar 

  22. Mitchell, P. J., Wang, C. & Tjian, R. Cell 50, 847–861 (1987).

    Article  CAS  Google Scholar 

  23. Sturm, R., Baumruker, T., Franza, B. R. & Herr, W. Genes Devel. (in the press).

  24. Briggs, M. R., Kadonaga, J. T. Bell, S. P. & Tjian, R. Science 234, 47–52 (1986).

    Article  ADS  CAS  Google Scholar 

  25. Kadonaga, J. T. & Tjian, R. Proc. natn. Acad. Sci. U.S.A 83, 5889–5893 (1986).

    Article  ADS  CAS  Google Scholar 

  26. Brady, J., Loeken, M. R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 82, 7299–7303 (1985).

    Article  ADS  CAS  Google Scholar 

  27. Takahashi, K. et al. Nature 319, 121–126 (1986).

    Article  ADS  CAS  Google Scholar 

  28. Dynan, W. S. & Tjian, R. Cell 32, 669–680 (1983).

    Article  CAS  Google Scholar 

  29. Salzman, N. P., Venkatachala, N. & Selzer, G. B. in The Papovaviridae, Vol. 1, The Polyomaviruses (ed. Salzman N. P.) 27–98 (Plenum, New York, 1986).

    Google Scholar 

  30. Brady, i. N., Radonovich, M. & Salzman, N. P. J. Virol. 44, 772–781 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  31. Rio, D. C. & Tjian, R. J. Molec. appl Genet. 2, 423–435 (1984).

    CAS  Google Scholar 

  32. Dynan, W. S. & Tjian, R. Cell 35, 79–87 (1983).

    Article  CAS  Google Scholar 

  33. Kim, C. H., Heath, C., Bertuch, A. & Hansen, U. Proc. natn. Acad. Sci. U.S.A. 84, 6025–6029 (1987).

    Article  ADS  CAS  Google Scholar 

  34. Nir, U., Fodor, E. & Rutter, W. J. Molec. cell. Biol. (in the press).

  35. Gallo, G. J. & Alwine, J. C. Molec. cell. Biol. (in the press).

  36. Fujimura, F. K., Deininger, P. L., Friedmann, T. & Linney, E. Cell. 23, 809–814 (1981).

    Article  CAS  Google Scholar 

  37. Karin, M., Haslinger, A., Heguy, A., Dietlin, T. & Cooks, T. Molec. cell. Biol. 7, 606–613 (1987).

    Article  CAS  Google Scholar 

  38. Comb, M., Birnberg, N. C., Seasholtz, A., Herbert, E. & Goodman, H. M. Nature 323, 353–356 (1986).

    Article  ADS  CAS  Google Scholar 

  39. Weaver, R. F. & Weissman, C. Nucleic Acids Res. 7, 1175–1193 (1979).

    Article  CAS  Google Scholar 

  40. Hanahan, D. J. molec. Biol. 166, 557–580 (1983).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry and Howard Hughes Medical Institute, University of California, Berkeley, California, 94720, USA

    Nicolas Mermod, Trevor J. Williams & Robert Tjian

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  1. Nicolas Mermod
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  2. Trevor J. Williams
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Mermod, N., Williams, T. & Tjian, R. Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro. Nature 332, 557–561 (1988). https://doi.org/10.1038/332557a0

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