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Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro

Nature volume 332, pages 557–561 (1988)Cite this article

Abstract

The simian virus 40 (SV40) transcriptional enhancer is composed of multiple cis-acting DNA sequence motifs, each individually having a two-to fourfold effect on the efficiency of transcription1–8. When various distinct cis-elements act in combination, however, a dramatic enhancement of transcription initiation often results9–11. SV40-enhancer A-domain sequences were previously shown to be important for early and late transcription in vivo. Here we report the isolation of the enhancer binding factor AP-4, which recognizes a motif in this domain. Purified AP-4 activates SV40 late transcription in vitro, and this stimulation is augmented by the addition of transcription factor AP-1 which binds to adjacent sequences in the A-domain12, suggesting coordinate action of the two factors for transcriptional enhancement. AP-1 also represses late transcription from a major in vitro start site which is poorly used in vivo, indicating that AP-1 can act as both a positive and negative regulator of SV40 late transcription. Thus by manipulating the levels of different trans-acting factors in vitro, we can recreate the pattern of SV40 late initiation observed during the viral lytic cycle in vivo.

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Affiliations

  1. Department of Biochemistry and Howard Hughes Medical Institute, University of California, Berkeley, California, 94720, USA

    Nicolas Mermod, Trevor J. Williams & Robert Tjian

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  1. Nicolas Mermod
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  2. Trevor J. Williams
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  3. Robert Tjian
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Mermod, N., Williams, T. & Tjian, R. Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro. Nature 332, 557–561 (1988). https://doi.org/10.1038/332557a0

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  • DOI: https://doi.org/10.1038/332557a0

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