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Reshaping human antibodies for therapy

Nature volume 332, pages 323–327 (1988)Cite this article

Abstract

A human IgGI antibody has been reshaped for serotherapy in humans by introducing the six hypervariable regions from the heavy- and light-chain variable domains of a rat antibody directed against human lymphocytes. The reshaped human antibody is as effective as the rat antibody in complement and is more effective in cell-mediated lysis of human lymphocytes.

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References

  1. Behring, E. von & Kitasato, S. Dtsch. Med. Wochenschr. 16, 1113–1114 (1890).

    Article  Google Scholar 

  2. Ratner, B. Allergy Anaphylaxis and Immunotherapy (Williams and Wilkins, Baltimore, 1943).

    Book  Google Scholar 

  3. Kohler, G. & Milstein, C. Nature 256, 495–497 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Ehrlich, P. in Immunology and Cancer Research vol. 2 (ed. Himmelweit, F.) (Pergamon, London, 1957).

    Google Scholar 

  5. Deland, F. H. & Goldenberg, D. M. in Radionuclide Imaging (ed. Kuhl, D. E.) 289–307 (Pergamon, Paris, 1982).

    Google Scholar 

  6. Levy, R. & Miller, R. A. A. Rev. Med. 34, 107–116 (1983).

    Article  CAS  Google Scholar 

  7. Miller, R. A., Oseroff, A. R., Stratte, P. T. & Levy, R. Blood 62, 988–995 (1983).

    CAS  PubMed  Google Scholar 

  8. Leung, D. Y. M., Rosen, & Geha, R. S. in Primary and Secondary Immunodeficiency Disorders (ed. Chandra, R. K.) 44–61 (Churchill Livingstone, New York, 1983).

    Google Scholar 

  9. Carson, D. A. & Freimark, B D. Adv. Immun. 38, 275–311 (1986).

    Article  CAS  PubMed  Google Scholar 

  10. Morrison, S. L., Johnson, M. J., Herzenberg, S. A. & Oi, V. T. Proc. natn. Acad. Sci. U.S.A. 81, 6851–6855 (1984).

    Article  ADS  CAS  Google Scholar 

  11. Boulianne, G. L., Hozumi, N. & Shulman, M. J. Nature 312, 643–646 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Neuberger, M. S. et al. Nature 314, 268–270 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Liu, A. Y. et al. Proc. natn. Acad. Sci. U.S.A. 84, 3439–3443 (1987).

    Article  ADS  CAS  Google Scholar 

  14. Shaw, D. R. et al. J. Immun. 138, 4534–4538 (1987).

    CAS  PubMed  Google Scholar 

  15. Bruggemann, M. et al. J. exp. Med. 166, 1351–1361 (1987).

    Article  CAS  PubMed  Google Scholar 

  16. Hale, G., Swirsky, D. M., Hayhoe, F. G. J. & Waldmann, H. Molec. biol. Med. 1, 321–334 (1983).

    CAS  PubMed  Google Scholar 

  17. Giorgi, J. V. et al. Transplantn Proc. 15, 639–642 (1983).

    Google Scholar 

  18. Benjamin, R., Cobbold, S. P., Clark, M. R. & Waldmann, H. J. exp. Med. 163, 1539–1552 (1986).

    Article  CAS  PubMed  Google Scholar 

  19. Jones, P. T., Dear, P. H., Foote, J., Neuberger, M. S. & Winter, G. Nature 321, 522–525 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Hale, G. et al. Blood 62, 873–882 (1983).

    CAS  PubMed  Google Scholar 

  21. Hale, G., Hoang, T., Prospero, T., Watt, S. M. & Waldmann, H. Molec. biol. Med. 1, 305–319 (1983).

    CAS  PubMed  Google Scholar 

  22. Hale, G. et al. J. Immun. Meth. 103, 59–67 (1987).

    Article  CAS  Google Scholar 

  23. Hale, G., Waldmann, H., Friend, P. & Calne, R. Transplantation 42, 308–311 (1986).

    Article  CAS  PubMed  Google Scholar 

  24. Hale, G., Swirsky, D. M., Hayhoe, F. G. J. & Waldmann, H. Molec. biol. Med. 1, 321–334 (1983).

    CAS  PubMed  Google Scholar 

  25. Kabat, E. A., Wu, T. T., Reid-Miller, M., Perry, H. M. & Gottesman, K. S. in Sequences of Proteins of Immunological Interest (US Dept. of Health and Human Services, US Government Printing Office, 1987).

    Google Scholar 

  26. Chothia, C., Novotny, J., Bruccoleri, R. & Karplus, M. J. J. molec. Biol. 186, 651–663 (1985).

    Article  CAS  PubMed  Google Scholar 

  27. Saul, F. A., Amzel M. & Poljak, R. J. J. biol. Chem. 253, 585–597 (1978).

    CAS  PubMed  Google Scholar 

  28. Epp, O. et al. Eur. J. Biochem. 45, 513–524 (1974).

    Article  CAS  PubMed  Google Scholar 

  29. Mulligan, R. C. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 78, 2072–2076 (1981).

    Article  ADS  CAS  Google Scholar 

  30. Southern, P. J. & Berg, P. J. molec. appl. Genetics 1, 327–3451 (1981).

    Google Scholar 

  31. Galfre, G. & Milstein, C. Meth. Enzym. 73, 1–46 (1981).

    Google Scholar 

  32. Chothia, C. & Lesk, A. J. Mol. Biol. 196, 901–917 (1987).

    Article  CAS  PubMed  Google Scholar 

  33. Roberts, S., Cheetham, J. C. & Rees, A. R. Nature 328, 731–734 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Junker, M. & den Brok, J. H. A. M. Eur. J. Immun. 17, 1547–1552 ( 1987).

    Article  Google Scholar 

  35. Clark, M. & Waldmann, H. J.N.C.I. 79, 1393–1398 (1987).

    CAS  Google Scholar 

  36. Hieter, P. A., Max, E. E., Seidmann, J. G., Maizel, J. V. Jr & Leder, P. Cell 22, 197–207 (1980).

    Article  CAS  PubMed  Google Scholar 

  37. Kaartinen, M. et al. J. Immun. 130, 320–324 (1983).

    Google Scholar 

  38. Gubier, U. & Hoffmann, B. J. Gene 25, 263–269 (1983).

    Article  Google Scholar 

  39. Sanger, F., Nicklen, S. A. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  40. Takahashi, N., Ueda, N. S., Obata, M., Nikaido, T. & Honjo, T. Cell 29, 671–679 (1982).

    Article  CAS  PubMed  Google Scholar 

  41. Flanagan, J. G. & Rabbits, T. H. Nature 300, 709–713 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  42. Huck, S., Fort, P., Crawford, D. H., Lefranc, M.-P. & Lefranc, G. Nucleic Acids Res. 14, 1779–1789 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Bruggemann, M. et al. Proc. natn. Acad. Sci. U.S.A. 83, 6075–6079 (1986).

    Article  ADS  CAS  Google Scholar 

  44. Potter, H., Weir, L. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 81, 7161–7163 (1984).

    Article  ADS  CAS  Google Scholar 

  45. Marquardt, M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 141, 369–391 (1980).

    Article  Google Scholar 

  46. Hale, G., Clark, M. & Waldmann, H. J. Immun. 123, 3056–3061 (1985).

    Google Scholar 

  47. Verhoeyen, M., Milstein, C. & Winter, G. Science (in the press).

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Author information

Author notes

  1. Michael Clark and Herman Waldmann: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK

  2. Lutz Riechmann: Address from April 1988: Department of Molecular Biology, The Research Institute of Scripps Clinic, North Torrey Pines Road, La Jolla, California 02937, USA

  3. Greg Winter: To whom correspondence should be addressed.

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Lutz Riechmann, Michael Clark, Herman Waldmann & Greg Winter

Authors
  1. Lutz Riechmann
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  2. Michael Clark
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  3. Herman Waldmann
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  4. Greg Winter
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Riechmann, L., Clark, M., Waldmann, H. et al. Reshaping human antibodies for therapy. Nature 332, 323–327 (1988). https://doi.org/10.1038/332323a0

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  • DOI: https://doi.org/10.1038/332323a0

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