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Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase

Nature volume 331, pages 190–192 (1988)Cite this article

Abstract

All eukaryotic cells so far analysed contain 19S particles which share a cylinder-like shape and are composed of a set of proteins of relative molecular mass ranging typically from 19,000 to 36,000 (refs 1–10). Proposed functions have included synthetase activity11, transfer RNA processing12 or messenger RNA repression6, but their biological importance remains obscure. A multicatalytic proteinase (MCP) of similar size and shape has been isolated from mammalian tissues13–24. The apparent similarities of these high molecular weight complexes suggest a biochemical and functional homology between the small cytoplasmic 19S particle from Drosophila melanogaster (19S-scRNP) (ref. 7) and rat MCP (ref. 14). By means of electron microscopy, immunological techniques, RNA identification and proteinase activity assays, we were able to show that the two structurally similar complexes are immunologically related ribonucleoproteins (RNPs) with similar proteolytic activity.

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Author notes

  1. Barbara Niedel, Friedrich Kopp, Lothar Kuehn and Burkhardt Dahlmann: Biochemische Abteilung, Diabetes Forschungs institut, Auf'm Hennekamp 65,D-4000 Düsseldorf, FRG

Affiliations

  1. ZMBH/Institut fÜr molekulare Genetik, Im Neuenheimer Feld 282,University of Heidelberg, D-6900, Heidelberg, FRG

    Patricia-E. Falkenburg, Christian Haass, Peter-M. Kloetzel, Barbara Niedel, Friedrich Kopp, Lothar Kuehn & Burkhardt Dahlmann

Authors
  1. Patricia-E. Falkenburg
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  2. Christian Haass
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  3. Peter-M. Kloetzel
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  4. Barbara Niedel
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  5. Friedrich Kopp
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  6. Lothar Kuehn
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  7. Burkhardt Dahlmann
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Falkenburg, PE., Haass, C., Kloetzel, PM. et al. Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase. Nature 331, 190–192 (1988). https://doi.org/10.1038/331190a0

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