Abstract
The leukaemic cells of more than 90% of chronic myelogenous leukaemia (CML) patients and of 10% of acute lymphocytic leukaemia (ALL) patients carry the t(9:22) (q34:qll) translocation which generates the Philadelphia chromosome (Ph1). In CML the abl gene is translocated from chromosome 9 to the centre of the bcr gene on chromosome 22 and this results in production of chimaeric bcr-abl RNA translated into a protein of relative molecular mass (Mr) 210,000 (210K). Our data indicate that in ALL abl is translocated into the 5' region of the bcr gene. The consequence of this is the expression of a fused transcript in which the first exon of bcr is linked to the second abl exon. This transcript encodes a 190K protein kinase.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Nowell, P. C. & Hungerford, D. A. Science 132, 1197-1200 (1960). 2. de Klein, A. et al. Nature 300, 765-767 (1982). 3. Groffen, J. et al. Cell 36, 93-99 (1984). 4. Heisterkamp, N., Stam, K., Groffen, J., de Klein, A. & Grosveld, G. Nature 315, 758-761 (1985). 5. Canaani, E. et al. Lancet i, 593-595 (1984). 6. Shtivelman, E., Lifshitz, B., Gale, R. P. & Canaani, E. Nature 315, 550-554 (1985). 7. Grosveld, G. et al. Molec. cell Biol. 6, 607-616 (1986). 8. Konopka, J. B., Watanabe, S. M. & Witte, O. N. Cell 37, 1035-1042 (1984). 9. Shtivelman, E. et al. Blood 69, 971-973 (1987). 10. Konopka, J. B., Watanabe, S. M., Singer, J. W., Collins, S. J. & Witte, O. N. Proc. natn. Acad. Sci. U.S.A. 82, 1810-1814 (1985). 11. Maxwell, S. A. et al. Cancer Res. 47, 1731-1739 (1987). 12. Catoski, D. Br. J. Haemal. 42, 493-498 (1979). 13. Erikson, J. et al. Proc. natn. Acad. Sci. U.S.A. 83, 1807-1811 (1986). 14. Clark, S. S., McLaughlin, J., Crist, W. M., Champlin, R. & Witte, O. N. Science 235, 85-88 (1987). 15. Kurzrock, R. et al. Nature 325, 631-635 (1987). 16. Chan, L. C. et al. Nature 325, 635-637 (1987). 17. Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning: A Practical Approach (ed. Glover, D.) 49-78 (IRL, Oxford, 1985). 18. Shtivelman, E., Lifshitz, B., Gale, R. P., Roe, B. A. & Canaani, E. Cell 47, 277-284 (1986). 19. Mes-Masson, A.-M., McLaughlin, J., Daley, G. Q., Paskind, M. & Witte, O. N. Proc. natn. Acad. Sci. U.S.A. 83, 9768-9772 (1986). 20. Hariharan, I. K. & Adams, J. M. EMBO J. 6, 115-119 (1987). 21. Lifshitz, B., Fainstein, E., Shtivelman, E., Gale, R. P. & Canaani, E. in Recent Advances in Leukemia and Lymphoma (eds Gale, R. P. & Golde, D. W.) UCLA Symp. Vol. 61 (Liss, New York, in the press). 22. Spindler, K. R., Rosser, D. S. E. & Berk, A. J. /. ViroL 49, 132-141 (1984). 23. Konopka, J. B. & Witte, O. Biochim. biophys. Acta 823, 1-17 (1985). 24. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560-564 (1977). 25. Greenberg, M. & Edelman, G. Cell 33, 767-779 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fainstein, E., Marcelle, C., Rosner, A. et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330, 386–388 (1987). https://doi.org/10.1038/330386a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/330386a0
This article is cited by
-
FISH-negative BCR::ABL1-positive e19a2 chronic myeloid leukaemia: the most cryptic of insertions
BMC Medical Genomics (2023)
-
HUGO Gene Nomenclature Committee (HGNC) recommendations for the designation of gene fusions
Leukemia (2021)
-
SHP2 is required for BCR-ABL1-induced hematologic neoplasia
Leukemia (2018)
-
Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
Scientific Reports (2017)
-
cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit
Biological Procedures Online (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.