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Immunization against MUC18/MCAM, a novel antigen that drives melanoma invasion and metastasis

Gene Therapy volume 14, pages 316–323 (2007)Cite this article

A Corrigendum to this article was published on 06 February 2007

Abstract

Melanoma patients with metastases have a very low survival rate and limited treatment options. Therefore, the targeting of melanoma cells when they begin to invade and metastasize would be beneficial. An adhesion molecule that is upregulated at the vertical growth phase is the melanoma cell adhesion molecule (MCAM/MUC18). MUC18 is expressed in late primary and metastatic melanoma with little or no expression on normal melanocytes. We utilized the alphavirus-based DNA plasmid, SINCp, encoding murine MUC18 (SINCp c-muMUC18) for vaccination against B16F10 murine melanoma cells expressing murine MUC18. This vaccine effectively protected mice from lethal challenges with melanoma-expressing murine MUC18 in both primary and metastatic tumor models. Vaccination against MUC18 elicited effective humoral and CD8+ T-cell immune responses against melanoma. We propose that targeting molecules important in tumor invasion may be useful in the design of future strategies for the prevention and treatment of melanoma.

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Acknowledgements

We thank Dr Yong-Jun Liu for critically reading the paper and Mr Walter Pagel for scientific editing. We also thank Chiron for providing the SINCp construct. This work was supported by NIH Grants CA 76098 and p50 CA 093459 (MB-E).

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Authors and Affiliations

  1. Department of Cancer Biology, Unit 173, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    M C Leslie, Y-J Zhao & M Bar-Eli

  2. Department of Experimental Therapeutic, Unit 173, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    L B Lachman

  3. Department of Medical Oncology, Unit 173, University of Texas, MD Anderson Cancer Center, Houston, TX, USA

    P Hwu

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  1. M C Leslie
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  2. Y-J Zhao
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  3. L B Lachman
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  5. M Bar-Eli
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Correspondence to M Bar-Eli.

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Leslie, M., Zhao, YJ., Lachman, L. et al. Immunization against MUC18/MCAM, a novel antigen that drives melanoma invasion and metastasis. Gene Ther 14, 316–323 (2007). https://doi.org/10.1038/sj.gt.3302864

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  • DOI: https://doi.org/10.1038/sj.gt.3302864

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