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Adenovirus-mediated VEGF165 gene transfer enhances wound healing by promoting angiogenesis in CD1 diabetic mice

Gene Therapy volume 9, pages 1271–1277 (2002)Cite this article

Abstract

It has been previously shown that vascular endothelial growth factor (VEGF) plays a central role in promoting angiogenesis during wound repair and that healing-impaired diabetic mice show decreased VEGF expression levels. In order to investigate the potential benefits of gene therapy with growth factors on wound repair, a replication-deficient recombinant adenovirus vector carrying the human VEGF165 gene (AdCMV.VEGF165) was topically applied on excisional wounds of streptozotocin-induced diabetic mice. Treatment with AdCMV.VEGF165 significantly accelerated wound closure when compared with AdCMV.LacZ-treated, as well as saline-treated control mice, by promoting angiogenesis at the site of injury. Our findings suggest that AdCMV.VEGF165 may be regarded as a therapeutic tool for the treatment of diabetic ulcers.

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Author notes

  1. S Romano Di Peppe and A Mangoni: The first two authors contributed equally to this work

Authors and Affiliations

  1. Laboratorio di Patologia Vascolare, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy

    S Romano Di Peppe, G Spinetti, G Melillo, M Napolitano & M C Capogrossi

  2. Laboratorio di Biologia Molecolare e Cellulare, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy

    G Zambruno

  3. Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Fondazione ‘I Monzino’, IRCCS, Milano, Italy

    A Mangoni

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  1. S Romano Di Peppe
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  2. A Mangoni
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  3. G Zambruno
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  5. G Melillo
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  7. M C Capogrossi
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Romano Di Peppe, S., Mangoni, A., Zambruno, G. et al. Adenovirus-mediated VEGF165 gene transfer enhances wound healing by promoting angiogenesis in CD1 diabetic mice. Gene Ther 9, 1271–1277 (2002). https://doi.org/10.1038/sj.gt.3301798

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  • DOI: https://doi.org/10.1038/sj.gt.3301798

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