Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Article
  • Published:

Gene transduction efficiency in cells of different species by HIV and EIAV vectors

Abstract

The ability of human immunodeficiency virus (HIV)- and equine infectious anaemia virus (EIAV)-based vectors to transduce cell lines from a range of species was compared. Both vectors carried the vesicular stomatitis virus G (VSV-G) envelope protein and encoded an enhanced green fluorescent protein (eGFP) gene driven by a human cytomegalovirus (CMV) early promoter. Immunostaining for viral core proteins and VSV-G was used to demonstrate that the HIV and EIAV vector preparations contained similar numbers of virus particles. Various cell lines were transduced with these vectors and the transduction efficiency was estimated by measuring eGFP expression. Efficient transduction by both vectors was observed in human, hamster, pig, horse, cat and dog cell lines, although EIAV vector was about 10-fold less efficient in human, hamster and pig cells normalised to the total number of viral particles. This could be partly explained by the lower RNA genome levels per particle for EIAV as measured by real-time RT-PCR. Rodent cells appeared to be transduced inefficiently with both vectors, but when the CMV promoter was substituted with the EF1α promoter in the HIV vectors, the expression level increased leading to an increase in the measurable level of transduction.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  1. Akkina RK et al. High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G J Virol 1996 70: 2581–2585

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Follenzi A et al. Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences Nat Genet 2000 25: 217–222

    Article  CAS  PubMed  Google Scholar 

  3. Naldini L et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector Science 1996 272: 263–267

    Article  CAS  PubMed  Google Scholar 

  4. Kotsopoulou EA et al. Rev-independent human immunodeficiency virus type 1 (HIV-1)-based vector that exploits a codon-optimized HIV-1 gag-pol gene J Virol 2000 74: 4839–4852

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Kim VN, Mitrophanous K, Kingsman SM, Kingsman AJ . Minimal requirement for a lentivirus vector based on human immunodeficiency virus type 1 J Virol 1998 72: 811–816

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Johnston JC et al. Minimum requirements for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors J Virol 1999 73: 4991–5000

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Wang G et al. Feline immunodeficiency virus vectors persistently transduce nondividing airway epithelia and correct the cystic fibrosis defect J Clin Invest 1999 104: 55–62

    Article  Google Scholar 

  8. Poeschla EM, Wong-Staal F, Looney DJ . Efficient transduction of nondividing human cells by feline immunodeficiency virus lentiviral vectors Nat Med 1998 4: 354–357

    Article  CAS  PubMed  Google Scholar 

  9. Curran MA, Kaiser SM, Achacoso PL, Nolan GP . Efficient transduction of nondividing cells by optimized feline immunodeficiency virus vectors Mol Ther 2000 1: 31–38

    Article  CAS  PubMed  Google Scholar 

  10. Mitrophanous K et al. Stable gene transfer to the nervous system using a non-primate lentiviral vector Gene Therapy 1999 6: 1808–1818

    Article  CAS  PubMed  Google Scholar 

  11. Olsen JC . Gene transfer vectors derived from equine infectious anemia virus Gene Therapy 1998 5: 1481–1487

    Article  CAS  PubMed  Google Scholar 

  12. Mazarakis ND et al. Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery Hum Mol Genet 2001 10: 2109–2121

    Article  CAS  PubMed  Google Scholar 

  13. Taube R et al. Interactions between equine cyclin T1, Tat and TAR are disrupted by a leucine-to-valine substitution found in human cyclin T1 J Virol 2000 74: 892–898

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Hofmann W et al. Species-specific, postentry barriers to primate immunodeficiency virus infection J Virol 1999 73: 10020–10028

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Zufferey R et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo Nat Biotechnol 1997 15: 871–875

    Article  CAS  PubMed  Google Scholar 

  16. Pizzato M, Marlow SA, Blair ED, Takeuchi Y . Initial binding of murine leukemia virus particles to cells does not require specific Env-receptor interaction J Virol 1999 73: 8599–8611

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Abe A, Chen ST, Miyanohara A, Friedmann T . In vitro cell-free conversion of noninfectious Moloney retrovirus particles to an infectious form by the addition of the vesicular stomatitis virus surrogate envelope G protein J Virol 1998 72: 6356–6361

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Sharma S, Miyanohara A, Friedmann T . Separable mechanisms of attachment and cell uptake during retrovirus infection J Virol 2000 74: 10790–10795

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Rohll JB et al. The design, production, safety, evaluation and clinical applications of non-primate lentiviral vectors Meth Enzymol 2002 346: 466–500

    Article  CAS  Google Scholar 

  20. Porter CD et al. Cationic liposomes enhance the rate of transduction by a recombinant retroviral vector in vitro and in vivo J Virol 1998 72: 4832–4840

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Neil S, Martin F, Ikeda Y, Collins M . Post-entry restriction to human immunodeficiency virus-based vector transduction in human monocytes J Virol 2001 75: 5448–5456

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by United Kingdom Medical Research Council and Cancer Research Campaign.

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ikeda, Y., Collins, M., Radcliffe, P. et al. Gene transduction efficiency in cells of different species by HIV and EIAV vectors. Gene Ther 9, 932–938 (2002). https://doi.org/10.1038/sj.gt.3301708

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.gt.3301708

Keywords

This article is cited by

Search

Quick links