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Efficient in vitro and in vivo gene regulation of a retrovirally delivered pro-apoptotic factor under the control of the Drosophila HSP70 promoter

Gene Therapy volume 8, pages 600–607 (2001)Cite this article

Abstract

We have developed a self-inactivating retroviral vector system with an internal, inducible Drosophila HSP70 promoter. This vector system delivers the desired transgene into cells rapidly and efficiently. It generates mixed populations of transduced cells where the transgene is inducible, and does not require the isolation of specific clones. Since the transgene is not expressed (or poorly expressed) at the restrictive condition (34°C), mixed populations can be selected in which tumor suppressors or other inhibitory genes can be strongly induced upon changing the conditions (39°C or the plant amino acid L-canavanine). This retroviral vector should be very useful for the expression of sequences that are poorly tolerated by cells, and is also active in animals.

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Acknowledgements

This work is supported by grant AG 16522 from the National Institutes of Health.

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  1. K Reiss

    Present address: Temple University, Philadelphia, PA 19122, USA

Authors and Affiliations

  1. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA

    G Romano

  2. Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA

    K Reiss, X Tu, F Peruzzi, B Belletti, J Y Wang, T Zanocco-Marani & R Baserga

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Romano, G., Reiss, K., Tu, X. et al. Efficient in vitro and in vivo gene regulation of a retrovirally delivered pro-apoptotic factor under the control of the Drosophila HSP70 promoter. Gene Ther 8, 600–607 (2001). https://doi.org/10.1038/sj.gt.3301441

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