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  • Viral Transfer Technology
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Photoactivatable retroviral vectors: a strategy for targeted gene delivery

Gene Therapy volume 7, pages 1999–2006 (2000)Cite this article

Abstract

We have explored a novel strategy for the targeting of retroviral vectors to particular sites or cell types. This strategy involves a method whereby the infectivity of a retroviral vector is neutralized by treatment of viral particles with a photocleavable, biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to long-wavelength UV light induces a reversal of the neutralizing, chemical modification resulting in restoration of infectivity to the viral vector. This infectivity ‘trigger’ possesses great potential, both as a research tool and as a novel tactic for the targeting of gene-transfer agents, since it would become possible to direct both the time and location of a viral infection in a versatile manner.

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Acknowledgements

We thank Kenneth J Rothschild and Jerzy Olejnik for useful discussions and providing photocleavable biotin samples, John C Guatelli and Jonathan Loeb for critical reading of the manuscript, Yasuhiro Takeuchi for the cell line TelCeB6, F-L Cosset for the plasmid pA, R Dornburg for pRD134, and Ruo Jie Wang and Muhammad-Omar Raiss for technical assistance. MWP is supported by a postdoctoral fellowship from the US Department of Army Prostate Cancer Research Program, PC990029.

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  1. Department of Radiology, The Center for Molecular Imaging Diagnosis and Therapy and Basic Science Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA

    MW Pandori & T Sano

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  1. MW Pandori
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  2. T Sano
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Pandori, M., Sano, T. Photoactivatable retroviral vectors: a strategy for targeted gene delivery. Gene Ther 7, 1999–2006 (2000). https://doi.org/10.1038/sj.gt.3301338

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