FACS analysis of infiltrated cells into injured cornea. Mice were treated as described in the legend to Figure 3. Five days after injury and gene transfer, the cells in the cornea were enzymatically isolated and analyzed using a flowcytometer (see Materials and methods). The cells were stained with anti-Mac-1 mAb and FITC-conjugated anti-F4/80 mAb for both macrophages. Gr-1 mAb stains were for neutrophils. Macrophages and neutrophils are supposed to be mac-1 high F4/80 high Gr-1 int or Mac-1 high F4/80 low Gr-1 high, respectively. (a) Total cell numbers per cornea; (b) A flowcytometric analysis using anti-Mac-1 and anti-Gr-1 antibodies and analysis using anti-Mac-1 and anti-F4/80 antibodies; (c) the number of neutrophils per cornea; and (d) that of macrophages per cornea are shown. The means ± s.d. (n = 12).