Figure 1 | Gene Therapy

Figure 1

From: Increasing endothelial cell specific expression by the use of heterologous hypoxic and cytokine-inducible enhancers

Figure 1

Enhancement of endothelial expression from the KDR promoter by hypoxic response elements after transient transfection and exposure to hypoxia. Skin endothelioma cells (sEND) and 3T3 fibroblasts were cotransfected with the Renilla luciferase reporter construct pRL-null containing the KDR or the HRE-KDR promoter and pGL3-control. Cells were incubated in normoxia or exposed to hypoxia (0.1% O2) for 16 h. The ratio of Renilla/firefly luciferase expression is shown (mean of three independent experiments, ± s.d.).

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