Abstract
Both ATP hydrolysis by myosin and the accompanying cyclic association–dissociation of actin and myosin are essential for muscle contraction. It is important for understanding the molecular mechanism of contraction to know the three-dimensional locations of the two major functional sites of myosin: the ATPase site1 and the actin-binding site2,3. We have determined the position of the ATPase site of myosin using three-dimensional image reconstruction from electron micrographs and site-specific labelling with the avidin–biotin system1. The ATPase site is about 5 nm from the tip of the myosin head and is about 4 nm away from the actin-binding site of myosin. This is the first report of the three-dimensional location of an enzyme active site by electron microscopy.
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References
Sutoh, K., Yamamoto, K. & Wakabayashi, T. Proc. natn. Acad. Sci. U.S.A. 83, 212–216 (1986).
Toyoshima, C. & Wakabayashi, T. J. Biochem., Tokyo 97, 245–263 (1985).
Tomioka, A., Tokunaga, M. & Wakabayashi, T. Proc. China-Japan Bilateral Symposium on Biophysics 505–509 (China Academic, 1985).
DeRosier, D. J. & Klug, A. Nature 217, 130–134 (1968).
Moore, P. B., Huxley, H. E. & DeRosier, D. J. J. molec. Biol. 50, 279–295 (1970).
Toyoshima, C. & Wakabayashi, T. J. Biochem., Tokyo 86, 1887–1890 (1979).
Wakabayashi, T. & Toyoshima, C. J. Biochem., Tokyo 90, 683–701 (1981).
Taylor, K. A. & Amos, L. A. J. molec. Biol. 147, 297–324 (1981).
Amos, L. A., Huxley, H. E., Holmes, K. C., Goody, R. S. & Taylor, K. A. Nature 299, 467–469 (1982).
Vibert, P. & Craig, R. J. molec. Biol. 157, 299–319 (1982).
Toyoshima, C. & Wakabayashi, T. J. Biochem., Tokyo 97, 219–243 (1985).
Sutoh, K., Yamamoto, K. & Wakabayashi, T. J. molec. Biol. 178, 323–339 (1984).
Toyoshima, C., Yamamoto, K., Sutoh, K., Sekine, T. & Wakabayashi, T. 11th YAMADA Conference, Abstr. p. 38 (1985).
Holtzman, E., Wise, D., Wall, J. & Karlin, A. Proc. natn. Acad. Sci. U.S.A. 79, 310–314 (1982).
Sutoh, K. Biochemistry (in the press).
Okamoto, Y. & Yount, R. G. Proc. natn. Acad. Sci. U.S.A. 82, 1575–1579 (1985).
Bayer, E. & Wilchek, M. Meth. Enzym. 34, 265–267 (1974).
Green, N. M., Konieczny, L., Toms, E. J. & Valentine, R. C. Biochem. J. 125, 781–791 (1971).
Elliott, A. & Offer, G. J. molec. Biol. 123, 505–519 (1978).
Takahashi, K. J. Biochem., Tokyo 83, 905–908 (1978).
Walker, M., Knight, P. & Trinick, J. J. molec. Biol. 184, 535–542 (1985).
Tao, T. & Lamkin, M. Biochemistry 20, 5051–5055 (1981).
Huxley, H. E. Science 164, 1356–1366 (1969).
Huxley, A. F. J. Physiol., Land. 243, 1–43 (1974).
Yagi, N., O'Brien, E. J. & Matsubara, I. Biophys. J. 33, 121–138 (1981).
Huxley, H. E., Faruqi, A. R., Kress, M., Bordas, J. & Koch, M. H. J. J. molec. Biol. 158, 637–684 (1982).
Yanagida, T. J. molec. Biol. 146, 539–560 (1981).
Cooke, R., Crowder, M. S. & Thomas, D. D. Nature 300, 776–778 (1982).
Borejdo, J., Assulin, O., Ando, T. & Putnam, S. J. molec. Biol. 158, 391–414 (1982).
Crowder, M., Wilkie, M. & Cooke, R. Biophys. J. 47, 381a (1985).
Weeds, A. G. & Taylor, R. S. Nature 257, 54–56 (1975).
DeRosier, D. J. & Moore, P. B. J. molec. Biol. 52, 355–369 (1970).
Amos, L. A. & Klug, A. J. molec. Biol. 99, 51–73 (1975).
Wakabayashi, T., Huxley, H. E., Amos, L. A. & Klug, A. J. molec. Biol. 93, 477–497 (1975).
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Tokunaga, M., Sutoh, K., Toyoshima, C. et al. Location of the ATPase site of myosin determined by three-dimensional electron microscopy. Nature 329, 635–638 (1987). https://doi.org/10.1038/329635a0
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DOI: https://doi.org/10.1038/329635a0
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