Letter | Published:

HIV-specific cytotoxic T lymphocytes in seropositive individuals

Abstract

Virus-specific cytotoxic T lymphocytes (CTL) which kill virus-infected cells are thought to be a major host defence against viral infections1–11. Here we report the existence of human immunodeficiency virus (HlV)-specific CTL in persons infected with this virus, the aetiological agent of AIDS (acquired immunodeficiency syndrome). Recombinant HIV-vaccinia viruses were used to express HIV antigens in B-cell lines established from subjects seropositive for HIV and seronegative controls. Circulating lymphocytes capable of killing HIV env-expressing autologous B cells were detected in eight of eight seropositive subjects; in addition, at least three seropositive subjects demonstrated gag-specific cytotoxic responses. No HIV-specific cytotoxicity was observed in seronegative subjects. Selective inhibition of the env-specific cytotoxicity by a CD3-specific monoclonal antibody indicates that the effectors are T cells. This demonstration of a cytotoxic T-cell immune response to HIV in infected individuals should prove useful in investigating the immunopathogenesis of HIV infection further and in evaluating AIDS vaccine strategies.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    1. Zinkernagel, R. M. & Welsh, R. M. J. Immun. 117, 1495–1502 (1976). 2. Yap, K. L., Ada, G. L. & McKenzie, I. F. C. Nature 273, 238–239 (1978). 3. Lin, Y–L. & Askonas, B. A. /. exp. Med. 154, 225–234 (1981). 4. Quinnan, G. V. et al. New Engl. J. Med. 307, 6–13 (1982). 5. McMichael, A. J., Gotch, F. M., Noble, G. R. & Beare, P. A. S. New Engl. J. Med. 309, 13–17 (1983). 6. Townsend, A. R. M. & McMichael, A. J. Prog. Allergy 36, 10–43 (1985). 7. Flyer, D. C., Burakoff, S. J. & Faller, D. V. Nature 305, 815–818 (1983). 8. Kannagi, M. et al. J. Immun. 130, 2942–2946 (1983). 9. Mitsuya, H. et al. J. exp. Med. 158, 994–999 (1983). 10. Van der Hoorn, F. A., Lahaye, T., Miiller, V., Ogle, M. A. & Enders, H. D. J. exp. Med. 162, 128–144 (1985). 11. Holt, C. A., Osorio, K. & Lilly, F. J. exp. Med. 164, 211–226 (1986). 12. Smith, G. L., Murphey, B. R. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 80, 7155–7159 (1983). 13. Bennick, J. R., Yewdell, J. W., Smith, G. L., Moller, C. & Moss, B. Nature 311, 578–579 (1984). 14. Yewdell, J. W., Bennick, J. R., Smith, G. L. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 82, 1785–1789 (1985). 15. Bennick, J. R, Yewdell, J. W., Smith, G. L. & Moss, B. /. Virol. 57, 786–791 (1986). 16. McMichael, A. J., Michie, C. A., Gotch, F. M., Smith, G. L. & Moss, B. / gen. Virol. 67, 719–726 (1986). 17. Zarling, J. M., Moran, P. A., Laskey, L. A. & Moss, B. J. Virol. 59, 506–509 (1986). 18. Earl, P. E. et al. Science 234, 728–731 (1986). 19. Chakrabarti, S., Robert–Guroff, M., Wong–Staal, F., Gallo, R. C. & Moss, B. Nature 320, 535–537 (1986). 20. Hu, S. L., Kosowski, S. G. & Dalrymple, J. M. Nature 320, 537–540 (1986). 21. Lifson, J. D. et al. Nature 323, 725–728 (1986). 22. Ratner, L. et al. Nature 313, 277–284 (1985). 23. Salahuddin, S. Z. et al. Virology 129, 51–64 (1983). 24. Blumberg, R. C. et alJ. infect. Dis. 155, 877–889 (1987). 25. Perrin, L. H., Zinkernagel, R. M. & Oldstone, M. B. A. J. exp. Med. 146, 949–969 (1977). 26. Wong–Staal, F. et al. Science 229, 759–762 (1985). 27. Starcich, B. R. et al. Cell 45, 637–648 (1986). 28. Townsend, A. R. M., McMichael A. J., Carter, N. P., Huddleston, J. A. & Brownlee, G. G. O//39, 13–25 (1984). 29. Yewdell, J. W. et al. J. exp. Med. 163, 1529–1538 (1986). 30. Ho, D. D. et al. Science 226, 451–453 (1984). 31. Essex, M. et al. Science 220, 859–862 (1983). 32. Lazarovits, A. I., Kurnick, J. T., Camerini, D., Karsh, J. & Colvin, R. B. in Leucocyte Typing ///, Proc. 3rd Int. Workshop on Leucocyte Differentiation Antigens (ed. McMichael A.) (Oxford Univ. Press, in the press).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.