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High-velocity microprojectiles for delivering nucleic acids into living cells

Nature volume 327, pages 7073 (07 May 1987) | Download Citation

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Abstract

We report here a novel phenomenon, namely that nucleic acids can be delivered into plant cells using high-velocity microprojec-tiles. This research was conducted in the hope of circumventing some of the inherent limitations of existing methods for delivering DNA into plant cells1–6. After being accelerated, small tungsten particles (microprojectiles) pierce cell walls and membranes and enter intact plant cells without killing them. Microprojectiles were used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically. This approach can therefore be used to study the transient expression of foreign genes in an intact tissue. It remains to be shown that smaller cell types, as are found in regenerable plant tissues, can be stably transformed by this method. If this proves possible, it would appear to provide a broadly applicable transformation mechanism capable of circumventing the host-range restrictions of Agrobacterium tumefaciens1, and the regeneration problems of protoplast transformation2–5.

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References

  1. 1.

    1. Fraley, R. T., Rogers, S. G. & Horsch, R. B. CRC crit. Rev. PL Sci. 4, 1–46 (1986). 2. Steinbiss, H. H. & Broughton, W. Int. Rev. Cytol. (Suppl.) 16, 191–207 (1983). 3. Lorz, H., Baker, B. & Schell, J. Molec. gen. Genet. 199, 178–182 (1985). 4. Potrykus, I., Saul, M. V., Petruska, J., Paszkowski, J. & Shillito, J. R. D. Molec. gen. Genet. 199, 183–188 (1985). 5. Fromm, M., Taylor, L. P. & Walbot, V. Proc. natn. Acad. Sci. U.S.A. 82, 5824–5828 (1985). 6. De la Pena, A., Lorz, H. & Schell, J. Nature 325, 274–276 (1987). 7. Sanford, J. C., Klein, T. M., Wolf, E. D. & Alien, N. Particle Sci. Technol. (in the press). 8. Christie, R. G. & Edwardson, J. R. Light and Electron Microscopy of Plant Virus Inclusions, Florida Agric. Exp. Station Monograph Ser. 9 (University of Florida, Gainesville, 1977). 9. Fukunaga, Y., Nagata, T. & Takebe, I. Virology 113, 752–760 (1981). 10. Fraley, R. T., Dellaporta, S. L. & Papahadjopoulos, D. Proc. natn. Acad. Sci. U.S.A. 79, 1859–1863 (1982). 11. Nishiguchi, M., Langridge, W. H. R., Szalay, A. A. & Zaitlin, M. PI. Cell Rep. 5,57–60 (1986). 12. Morelli, G., Nagy, F., Fraley, R. T., Rogers, S. G. & Chua, N.–H. Nature 315,200–204 (1985). 13. Crossway, A. et al. Molec. gen. Genet. 202, 179–185 (1986). 14. Brisson, N. & Hohn, T. Meth. Enzym. 118, 659–668 (1986). 15. Grimsley, N., Hohn, T., Davies, J. W. & Hohn, B. Nature 325, 177–179 (1987). 16. Hernalsteens, J.–P., Thia–Toong, L., Schell, J. & Van Montagu, M. EMBO J. 3, 3039–3041 (1984). 17. Hooykaas–Van Slogteren, G. M. S., Hooykaas, P. J. J. & Schilperoort, R. A. Nature 311, 763–764 (1984). 18. Ou–Lee, T. M., Turgeon, R. & Wu, R. Proc. natn. Acad. Sci. U.S.A. 83, 6815–6819 (1986). 19. Vasil, I. K. Int. Rev. Cytol. (Suppl.) 16, 79–88 (1983). 20. Potrykus, I. & Shillito, R. F. Meth. Enzym. 118, 549–578 (1986). 21. Zaitlin, M. in Nucleic Acids in Plants Vol. 2 (eds Hall, T. C. & Davis, J. W.) 31–64 (CRC Press, Boca Raton, Florida, 1979). 22. Coleman, A. W. & Goff, J. L. Stain Technol. 60, 145–154 (1985). 23. Hohn, T., Richards, K. & Lebeurier, G. Current Topics Microbiol. Immun. 96,193–236 (1982). 24. Alton, N. K. & Vapnek, D. Nature 282, 864–869 (1979). 25. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning, a Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982). 26. Gorman, C. M., Moffat, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).

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Affiliations

  1. Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell Univesity, Geneva, New York 14456, USA

    • T. M. Klein
    •  & J. C. Sanford
  2. Departments of Electrical Engineering Cornell University, Ithaca, New York 14453, USA

    • E. D. Wolf
  3. Departments of Biochemistry, Cornell University, Ithaca, New York 14453, USA

    • R. Wu

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https://doi.org/10.1038/327070a0

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