In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex1–3, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11)4 and Tp445,6) and murine (Thy-17–9, TAP10, and Ly-611) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti– variants12,13 of the human T-cell line Jurkat with the murine Thy-1.2 gene. Our data indicate that in CD3/Ti–, Thy-1.2+ transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti α- or β-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Chang, T. W., Kung, P. C., Gingras, S. P. & Goldstein, G. Proc. natn. Acad. Sci. U.S.A. 78, 1805–1808 (1982).
Reinherz, E. L. et al. Immun. Rev. 81, 95–129 (1984).
Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
Meuer, S. C. et al. Cell 36, 897–906 (1984).
Hara, T., Fu, S. M. & Hansen, J. A. J. exp. Med. 161, 1513–1524 (1985).
Moretta, A., Pantako, G., Lopez-Botet, M. & Moretta, L. J. exp. Med. 162, 823–838 (1985).
Gunter, K. C., Malek, T. R. & Shevach, E. M. J. exp. Med. 159, 716–730 (1984).
MacDonald, H. R., Bron, C., Rousseaux, M., Horvath, C. & Cerottini, J.-C. Eur. J. Immun. 15, 495–501 (1985).
Kroczek, R. A., Gunter, K. C., Seligmann, B. & Shevach, E. M. J. Immun. 136, 4379–4384 (1986).
Rock, K. L. et al. J. exp. Med. 163, 315–333 (1986).
Malek, T. R., Ortega, G., Chan, C., Kroczek, R. A. & Shevach, E. M. J. exp. Med. 164, 709–722 (1986).
Weiss, A. & Stobo, J. D. J. exp. Med. 160, 1284–1299 (1984).
Ohashi, P. S. et al. Nature 316, 606–609 (1985).
Gunter, K. C., Kroczek, R. A., Shevach, E. M. & Germain, R. N. J. exp. Med. 163, 285–300 (1986).
Kroczek, R. A., Gunter, K. C., Germain, R. N. & Shevach, E. M. Nature 322, 181–183 (1986).
Saito, T., Weiss, A., Miller, J., Norcross, M. & Germain, R. N. Nature 325, 125–130 (1987).
Weiss, A., Manger, B. & Imboden, I. J. Immun. 137, 819–825 (1986).
O'Flynn, K. et al. Eur. J. Immun. 16, 580–584 (1986).
June, C. H. et al. J. clin. Invest. 77, 1224–1232 (1986).
Low, M. G. & Kincade, P. W. Nature 318, 62–64 (1985).
About this article
Frontiers in Cell and Developmental Biology (2018)
Thy-1 stimulation of mouse T cells induces a delayed T cell receptor-like signal that results in Ca2+-independent cytotoxicity
Molecular Medicine Reports (2017)
Tumor Biology (2016)
Glycosylphosphatidylinositol (GPI)-anchored surface antigens in the allogeneic activation of T cells *
Clinical & Experimental Immunology (2008)
The Journal of Immunology (2006)